Lymph nodes and human tumors (review).

Prognostic Significance of CD169+ Lymph Node Sinus Macrophages in Patients with Malignant Melanoma

“…These observations suggest that monitoring or evaluating antitumor immunity may be useful for predicting the prognosis and outcome of anticancer therapy. In tumor immunology, the regional lymph nodes (RLN) that drain various malignant tumors, including malignant melanoma, are primary sites of the innate immune response (4). Fragmented dead tumor cells flow into the sinus area of the lymph node through lymphatic vessels (5) and are endocytosed by specific resident macrophages known as sinus macrophages.…”

Natural compounds that regulate lymph node sinus macrophages: Inducing an anti-tumor effect by regulating macrophage activation

“…7 In some malignant diseases, the LNs are thought to be important for anti-tumor immune responses because of the induction of cytotoxic T-lymphocytes (CTLs) into tumor tissue by APCs. 8,9 Sinus macrophages in the LN engulf antigens via several receptors, including scavenger receptors, and present antigen-derived peptides to T-and B-lymphocytes. 10,11 Asano et al demonstrated that CD169-positive subcapsular sinus macrophages in LNs are preferentially involved in antigen presentation and the induction of CTLs, and these CD169-positive macrophages are more significant for antitumor immune responses than the CD169 antigen itself.…”

Ex Vivo Stimulation of Tumor-Draining Lymph Node Cells from Lung Cancer Patients: A Potential Resource for Adoptive Immunotherapy

“…Li et al reported that after adoptive transfer of ex vivo IL-12 and IL-18 stimulated TDLN cells into mice bearing pulmonary metastases, the mean number of metastases was significantly reduced (4). Previous studies also showed that the transferred TDLN cells could accumulate in plexus vasculosus of alveolus, all the lymph nodes in the body and spleen, proliferate, and migrate into cancer tissue to inhibit tumor growth (5).…”

“…These target cells were collected and resuspended at 2 × 10 5 respectively. In addition, wells were set up to determine effector cell spontaneous lysis, target cell spontaneous lysis, target cell maximal release, medium alone, volume control and negative control wells (without stimulation).…”