Lymph Node Macrophages

Hoefsmit, E. C. M.; Kamperdijk, E. W. A.; Hendricks, H; Beelen, Robert H.J.; Balfour, Brigid M.

doi:10.1007/978-1-4899-5031-4_11

Cited by 13 publications

(15 citation statements)

References 81 publications

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“…In Figure 8B, an enlargement of this type of cell within the infiltrate shows characteristic features of the IDC. Such cells showed irregularly shaped nuclei with finely dispersed chromatin, translucent cytoplasm which contained small vesicles and narrow tubules, whorlshaped endoplasmic reticulum, and few lysosomes, as has been described previously (6,21,22,(32)(33)(34). It is significant that such IDC were present mainly in those transitional areas which contained increased numbers of lymphocytes.…”

Section: Resultssupporting

confidence: 78%

“…The IDC, as seen in the paracortex of lymphoid tissue, is a large cell with characteristic features: bizarre and often lobulated nucleus, clear cytoplasm, interdigitated plasma membrane, a tubulovesicular system, and typical whorl-shaped endoplasmic reticulum (21,22,32,33). However, even under the EM, the differentiation between macrophages and IDCs has proved difficult in synovial tissue, because the method of fixation used for monoclonal antibody staining (26) obscures the fine definition of the cellular organelles.…”

Section: Discussionmentioning

confidence: 99%

“…In the rheumatoid arthritis (RA) synovial membrane, Ia+ staining has been observed on lining layer cells and in perivascular mononuclear cell infiltrates (13)(14)(15)(16)(17) (21,22).…”

Section: Electron Microscopic Study Of Hla-dr and Monocyte/macrophagementioning

confidence: 99%

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Electron microscopic study of HLA‐DR and monocyte/macrophage staining cells in the rheumatoid synovial membrane

Iguchi

Kurosaka

Ziff

1986

Arthritis & Rheumatism

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We examined synovial membrane samples from 6 rheumatoid arthritis (RA) and 3 osteoarthritis patients and from 1 normal subject, by an immunoelectron microscopic technique using anti-HLA-DR (anti-Ia) and anti-monocyte/macrophage (63D3) monoclonal antibodies. In the lining layer, the type A macrophage-like cells were strongly DR+ and 63D3+, whereas the type B fibroblast-like cells were almost completely negative. Lymphocyte-rich areas (containing more than 90% densely packed lymphocytes) showed weak and patchy DR staining of the lymphocytes. In these areas, 3 4 % of the cells were macrophage-like cells which were 63D3-, a type of staining compatible with that of the interdigitating cell (IDC). In the plasma cell-containing (transitional) areas, many strongly DR+ macrophage-like cells were observed in close contact with lymphocytes and plasma cells. Ten to twenty percent of these cells were 63D3-, which suggests that they were IDC. Cells with the structural appearance of IDC were most frequently seen in those transitional areas which contained elevated concentrations (50-70%) of lymphocytes. In uninfiltratFrom the Harold C.

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Section: Resultssupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Electron microscopic study of HLA‐DR and monocyte/macrophage staining cells in the rheumatoid synovial membrane

Iguchi

Kurosaka

Ziff

1986

Arthritis & Rheumatism

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“…The reticular cells were closely associated with reticular fibers, enwrapping the fibers with their cell membrane. These cells corresponded to the cells designated as the "reticulum cell" (STUART, 1975;HOEFSMIT, 1975;KAMPERDIJK et al, 1978;HOEFSMIT et al, 1980;HENDRIKS et al, 1981) or "fibroblastic reticulum cell" (MULLER-HERMELINK and LENNERT, 1978).…”

Section: ) Reticular Cells (Figs La and 3a 2a And 3b)mentioning

confidence: 99%

“…These cells have been shown to come from the skin via lymphatics (KELLY et al, 1978). Interdigitating cells, which were first described as a type of reticular cell in the paracortex (VELDMAN, 1970), have been categorized as a type of migratory cell which has no contact with the reticular fiber (KAMPERDIJK et al, 1978;HOEFSMIT et al, 1980;HENDRIKS et al, 1981).…”

mentioning

confidence: 99%

Direct contact between reticular fibers and migratory cells in the paracortex of mouse lymph nodes. A morphological and quantitative study.

Hayakawa

Kobayashi

Hoshino

1988

Archives of Histology and Cytology

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Omental milky spots in the local immune response in the peritoneal cavity of rats

Vugt¹,

Rijthoven²,

Kamperdijk³

et al. 1996

Anat. Rec.

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Background Milky spots have been described as reactive structures, their classification varying from inflamed or haematopoietic tissue to lymphoid organs. In this study we investigated the reactivity of the milky spots in the omentum of rats upon induction of a chronic immune response in the peritoneal cavity. Methods At different time points after intraperitoneal administration of Bacillus Calmette‐Guérin (BCG), a peritoneal lavage was made, and the omentum and the draining parathymic lymph nodes were taken out. The cellular composition of these tissues was examined on the light microscopic level, using a panel of monoclonal antibodies, and also by electron microscopy. Results During the first 4 months after administering BCG, the number and size of the milky spots increased enormously. Separate macrophage, T, and B cell areas were formed, but interdigitating cells and follicular dendritic cells were not observed. The number of cells in the peritoneal cavity also increased, and the cellular composition showed a strong similarity with that of the milky spots. Especially during the onset of the experiment, most bacteria were observed in the macrophages in the milky spots rather than in the draining lymph nodes. A cellular immune response was observed in the parathymic lymph nodes but not in the milky spots. Conclusions Milky spots, either unstimulated or stimulated, should be classified as perivascular infiltrates. They play a role in the initial clearance of bacteria from the peritoneal cavity. Although the large increase in cell number is predominantly caused by immigration of cells, the results do support the role of milky spots as a site for local proliferation and maturation of especially macrophages and also B cells. The obtained data, however, do not support the earlier made assumption that milky spots function as a secondary lymphoid organ in the peritoneal cavity. © 1996 Wiley‐Liss, Inc.

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Lymph Node Macrophages

Cited by 13 publications

References 81 publications

Electron microscopic study of HLA‐DR and monocyte/macrophage staining cells in the rheumatoid synovial membrane

Electron microscopic study of HLA‐DR and monocyte/macrophage staining cells in the rheumatoid synovial membrane

Direct contact between reticular fibers and migratory cells in the paracortex of mouse lymph nodes. A morphological and quantitative study.

Omental milky spots in the local immune response in the peritoneal cavity of rats

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