Lyme Borreliosis, Po River Valley, Italy

Pistone, Dario; Pajoro, Massimo; Fabbi, Massimo; Vicari, Nadia; Marone, Piero; Genchi, C.; Novati, Stefano; Sassera, Davide; Epis, Sara; Bandi, Claudio

doi:10.3201/eid1608.100152

Cited by 22 publications

(14 citation statements)

References 11 publications

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“…In Italy, the reported B. burgdorferi s.l. prevalence in questing ticks varies considerably among study areas: from 17.5% to 20.6% in Veneto and Friuli Venezia Giulia (Favia et al., ; Rizzoli et al., ; Capelli et al., ); from 1.3% to 40.1% in Trentino Alto Adige (Cinco et al., ; Mantelli et al., ; Pecchioli et al., ; Nazzi et al., ); 18.0% in Lombardy (Pistone et al., ); 18.2% in Liguria (Mannelli et al., ); from 8.7% to 23.9% in Tuscany (Bertolotti et al., ; Selmi et al., ; Tomassone et al., ); 10.4% in Emilia‐Romagna (Corrain et al., ); 27.9% in Lazio (Cinco et al., ); 29,9% in Marche (Pascucci and Cammà, ). It is difficult to compare our results with those reported in the literature given differences in tick species collected, geographic locations and climatic conditions.…”

Section: Discussionmentioning

confidence: 98%

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Pintore

Ceballos

Iulini

et al. 2014

Zoonoses and Public Health

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Impacts• From 2008 to 2011, 11 human cases of Lyme Borreliosis were reported in the Verbano-Cusio-Ossola (VCO) province, north-eastern Piedmont, Italy.• The abundance and altitudinal distribution of ticks in VCO and B. burg dorferi s.l. in these vectors were examined. Phylogenetic analyses using ospC gene sequences were performed and 14 of 34 positive samples formed a clade with ospC reference sequences of human invasive strains.• The study supports the need for providing the right information to the institutions, physicians, forestry workers and visitors to adopt proper precautions during season at risk and to protect public health.

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Section: Discussionmentioning

confidence: 98%

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Pintore

Ceballos

Iulini

et al. 2014

Zoonoses and Public Health

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“…However, the risk of B. burgdorferi ( s.l. ) and other tick-borne infections is currently increasing in areas previously deemed unsuitable for Ixodes ticks, such as city parks [ 28 ], the Po River plain [ 29 ] and the western Alps [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

First detection of Borrelia miyamotoi in Ixodes ricinus ticks from northern Italy

et al. 2018

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BackgroundBorrelia miyamotoi is a spirochete transmitted by several ixodid tick species. It causes a relapsing fever in humans and is currently considered as an emerging pathogen. In Europe, B. miyamotoi seems to occur at low prevalence in Ixodes ricinus ticks but has a wide distribution. Here we report the first detection of B. miyamotoi in Ixodes ricinus ticks collected in two independent studies conducted in 2016 in the north-eastern and north-western Alps, Italy.ResultsThree out of 405 nymphs (0.74%) tested positive for Borrelia miyamotoi. In particular, B. miyamotoi was found in 2/365 nymphs in the western and in 1/40 nymphs in the eastern alpine area. These are the first findings of B. miyamotoi in Italy.ConclusionsExposure to B. miyamotoi and risk of human infection may occur through tick bites in northern Italy. Relapsing fever caused by Borrelia miyamotoi has not yet been reported in Italy, but misdiagnoses with tick-borne encephalitis, human granulocytic anaplasmosis or other relapsing fever can occur. Our findings suggest that B. miyamotoi should be considered in the differential diagnosis of febrile patients originating from Lyme borreliosis endemic regions. The distribution of this pathogen and its relevance to public health need further investigation.

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“…The presence of common tick-borne pathogenic bacteria [ 14 ] in the extracted DNA was screened using previously described PCR protocols for Borrelia burgdorferi [ 15 ], Anaplasma spp., Ehrlichia spp. and Rickettsia spp.…”

Section: Methodsmentioning

confidence: 99%

Ixodes ricinus and Its Endosymbiont Midichloria mitochondrii: A Comparative Proteomic Analysis of Salivary Glands and Ovaries

et al. 2015

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Hard ticks are hematophagous arthropods that act as vectors of numerous pathogenic microorganisms of high relevance in human and veterinary medicine. Ixodes ricinus is one of the most important tick species in Europe, due to its role of vector of pathogenic bacteria such as Borrelia burgdorferi and Anaplasma phagocytophilum, of viruses such as tick borne encephalitis virus and of protozoans as Babesia spp. In addition to these pathogens, I. ricinus harbors a symbiotic bacterium, Midichloria mitochondrii. This is the dominant bacteria associated to I. ricinus, but its biological role is not yet understood. Most M. mitochondrii symbionts are localized in the tick ovaries, and they are transmitted to the progeny. M. mitochondrii bacteria have however also been detected in the salivary glands and saliva of I. ricinus, as well as in the blood of vertebrate hosts of the tick, prompting the hypothesis of an infectious role of this bacterium. To investigate, from a proteomic point of view, the tick I. ricinus and its symbiont, we generated the protein profile of the ovary tissue (OT) and of salivary glands (SG) of adult females of this tick species. To compare the OT and SG profiles, 2-DE profiling followed by LC-MS/MS protein identification were performed. We detected 21 spots showing significant differences in the relative abundance between the OT and SG, ten of which showed 4- to 18-fold increase/decrease in density. This work allowed to establish a method to characterize the proteome of I. ricinus, and to detect multiple proteins that exhibit a differential expression profile in OT and SG. Additionally, we were able to use an immunoproteomic approach to detect a protein from the symbiont. Finally, the method here developed will pave the way for future studies on the proteomics of I. ricinus, with the goals of better understanding the biology of this vector and of its symbiont M. mitochondrii.

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Lyme Borreliosis, Po River Valley, Italy

Cited by 22 publications

References 11 publications

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

First detection of Borrelia miyamotoi in Ixodes ricinus ticks from northern Italy

Ixodes ricinus and Its Endosymbiont Midichloria mitochondrii: A Comparative Proteomic Analysis of Salivary Glands and Ovaries

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