Lycopersicon esculentum lectin is a marker of transient amplifying cells in in vitro cultures of isolated limbal stem cells

Vergallo, Cristian; Fonseca, T. S.; Pizzi, G.

doi:10.1016/j.tice.2010.05.001

Cited by 3 publications

(1 citation statement)

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“…Lectins have been used to characterize mouse embryonic stem cell cultures [Nash et al, 2007] as well as the features of Xenopus embryogenesis [Onuma et al, 2013] and for the in vitro identification of transient amplifying cells in cultures of isolated corneal epithelial stem cells [Vergallo et al, 2010]. Lectins have also been used to investigate metastatic processes in many cancer types [Miettinen et al, 1983;Hohenberger et al, 1990;Lalwani et al, 1996;Plattner et al, 2008] as well as to document the repertoire of glycoepitopes on the surface of embryonic carcinoma cells [Muramatsu et al, 1985] and as biomarkers for identifying glioma-derived stem cells [He et al, 2010;TuckerBurden et al, 2012].…”

Section: Aecsmentioning

confidence: 99%

Cell Surface Glycan Changes in the Spontaneous Epithelial-Mesenchymal Transition of Equine Amniotic Multipotent Progenitor Cells

Consiglio

Accogli

Crémonesi

et al. 2014

Cells Tissues Organs

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Amniotic epithelial cells (AECs) spontaneously transform into amniotic mesenchymal cells (AMCs) in vitro during cell culture. Glycocalyx was analyzed to identify the glycan pattern in AECs, AMCs and epithelial-mesenchymal transdifferentiated cells (EMTCs). Pure cell cultures were derived using cloned AEC and AMC cell lines obtained by the dilution technique from amniotic membranes. Mesenchymal cells generated by differentiation of clonal epithelial cells were considered transdifferentiated. Immunocytoscreen, in vitro multipotent differentiation and molecular characterization of EMTCs were performed. In combination with saponification and sialidase digestion, a panel of 12 lectins was used to analyze the glycan pattern of AEC, AMC and EMTC glycocalyx. Cytokeratin cell markers were lost in EMTCs and typical mesenchymal markers, such as vimentin, appeared. These cells retained their differentiation potential. Lectin histochemistry revealed a cell-specific glycan profile. Galactose (Gal)β1,4GlcNAc, Neu5Acα2,6Gal/GalNAc and N-acetyl neuraminic (sialic) acid (NeuNAc)α2,3Galβ1,3(±NeuNAcα2,6)GalNAc were highly expressed on the surface of all the amniotic cell cultures. AECs expressed asialoglycans with terminal GalNAc and GlcNAc. More highly mannosylated N-linked glycans and NeuNAcα2,3Galβ1,3GalNAc in O-linked glycans were expressed by EMTCs, but these cells had fewer glycans ending with fucose (Fuc), Gal, GlcNAc and GalNAc than AECs. GlcNAc- and GalNAc-terminating glycans were similarly expressed on the glycocalyx of the mesenchymal cell populations (EMTCs and AMCs). These results demonstrate for the first time that the spontaneous epithelial-mesenchymal transition (EMT) of equine amnion cells is characterized by cell surface glycan remodeling and that glycosylation changes result in a cell type-specific glycan profile. The glycopattern of equine amnion spontaneous EMTCs differs from EMT of tumoral cells.

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