Lycopene loaded whey protein isolate nanoparticles: An innovative endeavor for enhanced bioavailability of lycopene and anti-cancer activity

Jain, Ashay; Sharma, Gajanand; Ghoshal, Gargi; Kesharwani, Prashant; Singh, Bhupinder; Shivhare, U. S.; Katare, Om Prakash

doi:10.1016/j.ijpharm.2018.04.061

Cited by 120 publications

(42 citation statements)

References 42 publications

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“…Conclusively, the sustained release behavior of DSF from the hydrogel may be the reason for better cytotoxicity inside the cell. 56 Our cytotoxicity study results in the present research are better than the previously reported work. In various studies, the drug and polymer complexes significantly reduced the disadvantageous cytotoxicity of the antitumor drug and polymer complexes as the pharmacokinetic parameters of DDs control the fate of such drugs and polymer complexes.…”

Section: Resultscontrasting

confidence: 73%

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

2020

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A thermosensitive, physically cross-linked injectable hydrogel was formulated for the effective and sustained delivery of disulfiram (DSF) to the cancer cells as there is no hydrogel formulation available until now for the delivery of DSF. As we know, hydrogels have an advantage over other drug delivery systems because of their unique properties, so we proposed to formulate an injectable hydrogel system for the sustained delivery of an anticancer drug (DSF) to cancer cells. To investigate the surface morphology, a scanning electron microscope study was carried out, and for thermal stability of hydrogels, TGA (thermogravimetric analysis) and DSC (differential scanning calorimetry) were performed. The rheological behavior of hydrogels was evaluated with the increasing temperature and time. These developed hydrogels possessing excellent biocompatibility could be injected at room temperature following rapid gel formation at body temperature. The swelling index and in vitro drug release studies were performed at different pH (6.8 and 7.4) and temperatures (25 and 37 °C). The cell viability of the blank hydrogel, free DSF solution, and Ch/DSF (chitosan/DSF)-loaded hydrogel was studied by MTT assay on SMMC-7721 cells for 24 and 48 h, which exhibited higher cytotoxicity in a dose-dependent manner in contrast to the free DSF solution. Moreover, the cellular uptake of DSF-loaded hydrogels was observed stronger as compared with free DSF. Hence, chitosan-based hydrogels loaded with DSF possessing exceptional properties can be used as a novel injectable anticancer drug for the sustained delivery of DSF for long-term cancer therapy.

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Section: Resultscontrasting

confidence: 73%

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

2020

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“…Antitumoral effect of LYC-WPI-NPs and survival data indicate that proposed formulation strategy is a novel approach for the synchronized delivery of bioactive compound, leading to increased bioavailability, therapeutic efficacy, and safety profiling because of improvement in animal survival (100%), in contrast to animals free of LYC (66.67%) and negative control group (16.67%). This will certainly open new avenues to explore cancer treatment [49].…”

Section: Lycopene With Nanoparticlesmentioning

confidence: 99%

Cytotoxic Effect and Mechanisms from Some Plant-Derived Compounds in Breast Cancer

Pérez-Soto¹,

Estanislao-Gómez²,

Pérez-Ishiwara³

et al. 2019

Cytotoxicity - Definition, Identification, and Cytotoxic Compounds

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Breast cancer (BrC) is a major health problem in women all around the world. A growing knowledge about these alterations and their associated molecular signaling pathways offers opportunities for therapeutic strategies; chemotherapy is one of the most utilized treatments; however, because of the adverse side effects and multidrug resistance that patients may present, there has been great advancement in search of new alternatives as the use of plant-derived natural compounds. This review describes information on the progress and development of cytotoxic compounds against BrC belonging to the families of flavonoids, terpenes, and alkaloids that through in vitro and in vivo studies have demonstrated to induce cellular death mainly through apoptosis, activating the intrinsic pathway. The in vitro IC 50 and the in vivo EC 50 dose-response relationship can vary depending on various factors, including the choice of cell line and/or the model used. Also, the association of some of these compounds with nanoparticles or paclitaxel with antibodies has clearly shown a potential improvement in its effect. The clinical studies that are being conducted with some of them show promising results; however, it is necessary to continue with the effort to develop new and more effective drugs against different types of BrC.Cytotoxic Effect and Mechanisms from Some In vivo models for studying plant-derived compounds in breast cancerWide varieties of animal model systems are now available to investigate plantderived compounds in different stages, such as cancer initiation, promotion progression, invasion, and metastasis. These models are also used to comprehend therapeutic response, which represents an essential step between in vitro systems and clinical studies [8,13]. The in vivo models are also used to investigate the capability of plant formulation to induce an anti-BrC effect where it is sought to optimize dose, bioavailability, administration routes, and selective delivery and reduce toxic effects, among others [8,14]. The two animal species that will be mentioned in this review are those involving mice and rats [14]; however, BrC mouse models are used in a variety of preclinical studies [13].There are different types of in vivo models of BrC, such as cell line-derived xenografts (MDA-MB-231 line) that are implanted into immunocompromised animals (cell-derived xenografts, CDX). CDX models represent a relatively homogenous mass of transformed breast epithelial cells, and depending on where the cells are inoculated, they are classified as ectopic CDX (models advanced disease only, subcutaneous injection of human tumor cells), orthotopic CDX (in mammary gland/fat pad), metastatic CDX (following tail vein or intra-cardiac injection in specific sites, i.e., bone or lung), syngeneic (mouse tissue implanted to strainmatched host) or metastasis with syngeneic model (usually fast-growing tumors and microenvironment derive from the same species, i.e., 4T1 cells), and genetically engineered mouse models (GEMMs) to address early events of tu...

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“…The NPs prepared from whey protein have controlled release behavior, safer, easy to manufacture, and rapidly absorbent across biological membrane. WPI is an ideal candidate for the fabrication of novel DDs for lipophilic compounds due to its binding ability to hydrophobic constituents …”

Section: Application Of Whey Protein For Novel Ddsmentioning

confidence: 99%

“…They also investigated the cytotoxicity studies of WPI NPs against MCF‐7 breast cancer cells. These protein‐based NPs are considered efficient nanocarriers to improve biopharmaceutical attributes of lycopene, reduce tumor proliferation, and enhance the survival rate of treated animals . This group had focused on the utilization of WPI for the synthesis of NPs for the entrapment of zinc citrate.…”

Section: Application Of Whey Protein For Novel Ddsmentioning

confidence: 99%

Whey protein: A functional and promising material for drug delivery systems recent developments and future prospects

Farooq

Aquib

Ghayas

et al. 2019

Polymers for Advanced Techs

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Natural polymers have been extensively utilized in the past decades due to their outstanding features. Among these natural excipients, protein‐based polymers have superb features owing to their high drug binding capacity and biodegradability. Whey protein is a versatile protein‐based vehicle for drug delivery systems. It has been shown to be nontoxic, biocompatible, and biodegradable. Therefore, it has been considered as an ideal biomaterial for the design of advanced drug delivery systems. Protein‐based cargo acts as synthetic polymers counterpart for innovative delivery systems. The current review is mainly focused on application of whey proteins as an emerging carrier in drug delivery systems, achieved during the past.

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Lycopene loaded whey protein isolate nanoparticles: An innovative endeavor for enhanced bioavailability of lycopene and anti-cancer activity

Cited by 120 publications

References 42 publications

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

Cytotoxic Effect and Mechanisms from Some Plant-Derived Compounds in Breast Cancer

Whey protein: A functional and promising material for drug delivery systems recent developments and future prospects

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