Lycopene influences osteoblast functional activity and prevents femur bone loss in female rats submitted to an experimental model of osteoporosis

Oliveira, Gustavo R.; Sánchez, Paula Katherine Vargas; Fernandes, Roger Rodrigo; Ricoldi, Milla Sprone Tavares; Semeghini, Mayara Sgarbi; Pitol, Dimítrius Leonardo; Sousa, Luiz Gustavo de; Siéssere, Selma; Bombonato-Prado, Karina Fittipaldi

doi:10.1007/s00774-018-0970-8

Cited by 26 publications

(16 citation statements)

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“…The latter is one of the main carotenoids and is among the best-documented nutraceutical compounds with a significant health benefit. Our observations are in line with other previous findings demonstrating that a diet rich in carotenoids has a positive impact over the BMD [ 45 ] and that lycopene influences osteoblast differentiation [ 46 , 47 ]. Furthermore, Oscillospira abundance is associated with microbial diversity, leanness, and high levels of HDL [ 48 ]; meanwhile, Pantoea is a potentially pathogenic bacteria belonging to γ- Proteobacteria phylum and Enterobacteriaceae family associated with inflammatory responses [ 49 ].…”

Section: Discussionsupporting

confidence: 93%

A Multi-Omic Analysis for Low Bone Mineral Density in Postmenopausal Women Suggests a Relationship between Diet, Metabolites, and Microbiota

et al. 2020

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The effect of microbiota composition and its health on bone tissue is a novel field for research. However, their associations with bone mineral density (BMD) have not been established in postmenopausal women. The present study investigates the relation of diet, the microbiota composition, and the serum metabolic profile in postmenopausal women with normal-BMD or with low-BMD. Ninety-two Mexican postmenopausal women were classified into normal-BMD (n = 34) and low-BMD (n = 58). The V4 hypervariable region was sequenced using the Miseq platform. Serum vitamin D was determined by chemiluminescence immunoassay. Serum concentrations of acyl-carnitines and amino acids were determined by electrospray tandem mass spectrometry. Diet was assessed by a food frequency questionnaire. The low-BMD group had fewer observed species, higher abundance of γ-Proteobacteria, lower consumption of lycopene, and lower concentrations of leucine, valine, and tyrosine compared with the normal-BMD group. These amino acids correlated positively with the abundance of Bacteroides. Lycopene consumption positively correlated with Oscillospira and negatively correlated with Pantoea genus abundance. Finally, the intestinal microbiota of women with vitamin D deficiency was related to Erysipelotrichaceae and Veillonellaceae abundance compared to the vitamin D non-deficient group. Associations mediated by the gut microbiota between diet and circulating metabolites with low-BMD were identified.

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Section: Discussionsupporting

confidence: 93%

A Multi-Omic Analysis for Low Bone Mineral Density in Postmenopausal Women Suggests a Relationship between Diet, Metabolites, and Microbiota

et al. 2020

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“…Rankl stimulates osteoclastic activity and differentiation and inhibits osteoclast apoptosis whereas osteoprotegerin inhibits osteoclast formation by binding to Rankl (23). The higher expression of Opg in Ovx groups have also been observed in a previous report (14) with the use bone marrow osteoblastic cell source. In agreement to Yu et al (17), the removal of the ovaries reduces estrogen concentrations, leading to a need for initial enhancement of osteoblast function in order to compensate for the effects of the decrease in estrogen.…”

Section: Discussionsupporting

confidence: 85%

“…The MTT assay showed a lower cell proliferation in OVX group in all experimental periods. These results differ from other investigations that used cells from different sites such as bone marrow, when osteoblastic cells showed higher or similar cell proliferation in OVX groups when compared to sham group (14). Nevertheless, Semeghini et al (15) also showed lower cell proliferation of calvaria osteoblastic cells from female ovariectomized whereas bone marrow cells from sham and OVX groups had similar proliferation.…”

Section: Discussioncontrasting

confidence: 72%

Osteoporosis Affects Functional Activity and Gene Expression of Osteoblastic Cells Derived from Rat Alveolar Bone

et al. 2020

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Recent studies suggest that osteoporosis, in addition to the damage caused in long bones, may cause deterioration in the jaws, especially in alveolar bone sites, with effects in the progress of periodontal disease as well as in bone healing. The aim of this study was to evaluate the effect of osteoporosis in the metabolism of rat alveolar bone osteoblasts. There were used 10 female rats divided in two experimental groups (Sham and OVX), which were ovariectomized and after 8 weeks euthanized to collect mandibular bone samples in order to isolate osteoblastic cells. The cells were cultured in 24-well plates to perform the in vitro experiments. After 7, 10 and 14 days, there were evaluated cell proliferation by MTT assay, in situ detection of alkaline phosphatase (ALP) as well as mineralized nodules and expression of genes associated to bone remodeling. Results showed that at 7, 10 and 14 days cell proliferation was lower for OVX group. In situ detection of ALP was higher at 7 days and lower at 10 and 14 days in OVX group. At 17 and 21 days, OVX group had a significative decrease of mineralization nodules. There was a downregulation in the expression of Alp, Bglap and Runx2 genes and an upregulation of Opg in OVX group, whereas Opn and Rankl modulation was similar between the evaluated groups. Our results suggest that osteoporosis has a deleterious effect on alveolar bone cells from ovariectomized rats, which might affect the treatment of diseases associated to the jaw bones.

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“…In addition, all-trans-retinoic acid has been reported to induce the early osteoblastic transcription factor, RUNX2 expression [33]. Oliveira et al [34] found an upregulation of RUNX2 in lycopene supplemented ovariectomized rats. In line with these investigations, we found that lycopene, at both doses, increased RUNX2 mRNA levels ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Lycopene and bone: an in vitro investigation and a pilot prospective clinical study

et al. 2020

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Background: There are several effective therapies for osteoporosis but these agents might cause serious adverse events. Lycopene intake could prevent bone loss, however studies on its effects on bone are scarce. Our aim was to investigate the effects of lycopene on osteoblast cells as well as bone mineral density and bone turnover markers in postmenopausal women. Methods: We investigated the effect of lycopene on the Wnt/β-catenin and ERK 1/2 pathways, RUNX2, alkaline phosphatase, RANKL and COL1A of Saos-2. We also carried out a pilot controlled clinical study to verify the feasibility of an approach for bone loss prevention through the intake of a lycopene-rich tomato sauce in 39 postmenopausal women. Results: Lycopene 10 µM resulted in higher β-catenin and phERK1/2 protein Vs the vehicle (p = 0.04 and p = 0.006). RUNX2 and COL1A mRNA was induced by both 5 and 10 µM doses (p = 0.03; p = 0.03 and p = 0.03; p = 0.05) while RANKL mRNA was reduced (p < 0.05). A significant bone density loss was not detected in women taking the tomato sauce while the control group had bone loss (p = 0.002). Tomato sauce intake resulted in a greater bone alkaline phosphatase reduction than the control (18% vs 8.5%, p = 0.03). Conclusions: Lycopene activates the WNT/β-catenin and ERK1/2 pathways, upregulates RUNX2, alkaline phosphatase, COL1A and downregulates RANKL Saos-2. These processes contributed to prevent bone loss in postmenopausal women.

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Lycopene influences osteoblast functional activity and prevents femur bone loss in female rats submitted to an experimental model of osteoporosis

Cited by 26 publications

References 36 publications

A Multi-Omic Analysis for Low Bone Mineral Density in Postmenopausal Women Suggests a Relationship between Diet, Metabolites, and Microbiota

A Multi-Omic Analysis for Low Bone Mineral Density in Postmenopausal Women Suggests a Relationship between Diet, Metabolites, and Microbiota

Osteoporosis Affects Functional Activity and Gene Expression of Osteoblastic Cells Derived from Rat Alveolar Bone

Lycopene and bone: an in vitro investigation and a pilot prospective clinical study

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