Lycium ruthenicum diet alters the gut microbiota and partially enhances gut barrier function in male C57BL/6 mice

Tian, Baoming; Zhao, Jianhua; An, Wei; Zhang, Jiawei; Cao, Xin; Mi, Jiale; Zhao, Jing; Zhang, Yingxiao; Li, Juxiu

doi:10.1016/j.jff.2018.11.034

Cited by 32 publications

(36 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cecum contained abundantly gut microbiota and was an important place for microbial fermentation. [ 30,31 ] The size changes in cecum might affect the composition of gut microbiota. To further explore the effect of mEVs on gut microbiota composition, the colonic contents from C57BL/6 mice were collected and analyzed the microbial composition via 16S rRNA gene (v3‐v4 regions) sequencing.…”

Section: Resultsmentioning

confidence: 99%

Oral Administration of Bovine Milk‐Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice

Tong

Hao

Zhang

et al. 2020

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Scope Milk‐derived extracellular vesicles (mEVs) as nanoparticles are being developed as novel drug vehicles due to their pivotal role in cell–cell communication. As an important bioactive component in milk, little is known about their effect on the gut microbiota and intestinal immunity. Therefore, the effects of mEVs on gut microbiota and intestinal immunity in mice are investigated. Methods and results First, a new method to obtain high‐yield mEVs is developed. Afterward, the colonic contents from C57BL/6 mice fed different doses of mEVs (8 weeks) are collected and the microbial composition via 16S rRNA gene sequencing is analyzed. It is found that mEVs could alter the gut microbiota composition and modulate their metabolites—short‐chain fatty acids (SCFAs). Furthermore, the effects of mEVs on intestinal immunity are evaluated. It is observed that the expression levels of Muc2, RegIIIγ, Myd88, GATA4 genes, and IgA, sIgA are increased in the intestine, which are significant for the integrity of the mucus layer. Conclusion These findings reveal that the genes with critical importance for intestinal barrier function and immune regulation are modified in mice by oral administration mEVs, which also result in the changes of the relative composition of gut microbiome and SCFAs.

show abstract

Section: Resultsmentioning

confidence: 99%

Oral Administration of Bovine Milk‐Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice

Tong

Hao

Zhang

et al. 2020

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…Genus Ruminococcaceae UCG-014 from phylum Firmicutes is recognized as a SCFA producer that autochthonously inhabits in the cecum and colon ( Donaldson et al., 2016 ; Tian et al., 2019 ). Generally, members of Ruminococcaceae are equipped with endo-1,4-beta-xylanase and cellulase genes that are responsible for the degradation of diverse cellulose and hemicellulose components of plant material ( Biddle et al., 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides

Liu¹,

Li²,

Wang

et al. 2021

Poultry Science

View full text Add to dashboard Cite

This study was designed to evaluate the effects of γ-irradiated Astragalus polysaccharides ( IAPS ) on growth performance, cecal microbiota populations, and concentrations of cecal short-chain fatty acids of immunosuppressed broilers. A total of 144 one-day-old broiler chicks were randomly assigned into 3 groups: nontreated group (control), cyclophosphamide ( CPM )-treated groups fed either a basal diet or the diets containing 900 mg/kg IAPS, respectively. On day 16, 18, and 20, broilers in the control group were intramuscularly injected with 0.5 mL sterilized saline (0.75%, wt/vol), and those in the CPM and IAPS groups were intramuscularly injected with 0.5 mL CPM (40 mg/kg of BW). The trial lasted 21 d. Compared with the control group, CPM treatment decreased the broiler average daily gain ( ADG ) and feed intake ( P < 0.05) but did not affect the overall microbial diversity and compositions, as well as the concentrations of cecal acetate, propionate, and butyrate in cecum of broilers ( P > 0.05). Dietary IAPS supplementation increased broiler ADG, Shannon index, and decreased Simpson index ( P < 0.05). Specifically, broilers fed diets containing IAPS showed lower abundances of Faecalibacterium , Bacteroides, and Butyricicoccus and higher proportions of Ruminococcaceae UCG-014 , Negativibacillus , Shuttleworthia , Sellimonas, and Mollicutes RF39_norank, respectively ( P < 0.05). The IAPS treatment also increased butyrate concentration ( P < 0.05) and tended to elevate acetate concentration ( P = 0.052) in cecal digesta. The results indicated that IAPS are effective in increasing the cecal beneficial bacteria and short-chain fatty acids production, contributing to improvement in the growth performance of immunosuppressive broilers. These findings may expand our knowledge about the function of modified Astragalus polysaccharides in broiler chickens.

show abstract

“…Mucispirillum inhabits the intestinal mucous layer in rodents and humans, has been suggested to be a pathobiont, is not an SCFA-producing bacteria, and may utilise SCFAs for energy metabolism (Loy et al, 2017). According to our previous report, the abundance of Ruminiclostridium_9 gradually decreased with the increase in the additive amount of L. ruthenicum, which may be beneficial for the colonic barrier (Tian et al, 2019). Alistipes was highly prevalent in the most frail individuals and in those with major depressive disorder, and the most significantly overrepresented taxon in middle-aged and older mice (Tian et al, 2019).…”

Section: Gm Community Structure At the Phylum And Genus Levelsmentioning

confidence: 95%

Dietary whole Goji berry (Lycium barbarum) intake improves colonic barrier function by altering gut microbiota composition in mice

Tian

Zhang

Zhao

et al. 2020

Int J of Food Sci Tech

Self Cite

View full text Add to dashboard Cite

Diet strongly affects human health by modulating gut microbiota (GM) composition. Lycium barbarum (LB), which contains polysaccharides and polyphenols, can alter the GM and intestinal barrier function, thereby reducing and preventing the occurrence of intestinal diseases. To investigate how LB improves colonic barrier function, male C57BL/6J mice were fed diets containing 1.5% or 3% LB for 10 weeks. Results of HiSeq 16S rDNA analysis showed that LB markedly altered microbial profile by supporting the growth of Verrucomicrobia, Bacteroidetes, Bacteroidales_S24-7_group, Anaerotruncus, Coprococcus_1, Ruminococcaceae_UCG-014 and Akkermansia, while suppressing Firmicutes, Helicobacter, Bacteroides and Mucispirillum. LB supported the short-chain fatty acid (SCFA)-producing bacteria, and the SCFA concentrations increased, while the ammonia concentrations and pH values in faeces decreased, thereby increasing the expression of tight junction proteins and mucin, subsequently improving intestinal function and histomorphology. Dietary LB, as a functional dietary component, enhanced colonic barrier function and improved gut health by altering GM composition.

show abstract

Lycium ruthenicum diet alters the gut microbiota and partially enhances gut barrier function in male C57BL/6 mice

Cited by 32 publications

References 76 publications

Oral Administration of Bovine Milk‐Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice

Oral Administration of Bovine Milk‐Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice

Microbiota populations and short-chain fatty acids production in cecum of immunosuppressed broilers consuming diets containing γ-irradiated Astragalus polysaccharides

Dietary whole Goji berry (Lycium barbarum) intake improves colonic barrier function by altering gut microbiota composition in mice

Contact Info

Product

Resources

About