2012
DOI: 10.1002/iub.1048
|View full text |Cite
|
Sign up to set email alerts
|

Lyase activity of glycogen synthase: Is an elimination/addition mechanism a possible reaction pathway for retaining glycosyltransferases?

Abstract: Despite the biological relevance of glycosyltrasferases (GTs) and the many efforts devoted to this subject, the catalytic mechanism through which a subclass of this large family of enzymes, namely those that operate with net retention of the anomeric configuration, has not been fully established. Here, we show that in the absence of an acceptor, an archetypal retaining GT such as Pyrococcus abyssi glycogen synthase (PaGS) reacts with its glucosyl donor substrate, uridine 5'‐diphosphoglucose (UDP‐Glc), to produ… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
5

Citation Types

0
16
0

Year Published

2013
2013
2024
2024

Publication Types

Select...
6

Relationship

1
5

Authors

Journals

citations
Cited by 11 publications
(16 citation statements)
references
References 45 publications
0
16
0
Order By: Relevance
“…S10 in the supplemental material). This ligand mimics the planar structure of the transition state in either the S N i, S N i-like, or elimination/addition mechanism (56). This type of in situ-generated intermediate was also observed in the E. coli and P. abyssi glycogen synthases (25,56).…”
Section: Ii) Sugar-binding Gt-b(a) Domainmentioning
confidence: 63%
See 4 more Smart Citations
“…S10 in the supplemental material). This ligand mimics the planar structure of the transition state in either the S N i, S N i-like, or elimination/addition mechanism (56). This type of in situ-generated intermediate was also observed in the E. coli and P. abyssi glycogen synthases (25,56).…”
Section: Ii) Sugar-binding Gt-b(a) Domainmentioning
confidence: 63%
“…In either of those two cases, an important stabilization of the transition state would be based on the interaction between the anomeric carbon (C-1) of the sugar being transferred and the oxygen of the main chain of a His residue (24,55). Recently, an alternative elimination/addition mechanism has been proposed for the Pyrococcus abyssi glycogen synthase (56), which was argued to be compatible with the currently available data. In this mechanism, a general base is needed to extract a proton from C-2 of the glycosyl group.…”
Section: Ii) Sugar-binding Gt-b(a) Domainmentioning
confidence: 83%
See 3 more Smart Citations