Ly6e expression is restricted to syncytiotrophoblast cells of the mouse placenta

Hughes, Martha; Natale, Bryony V.; Simmons, David G.; Natale, David R.C.

doi:10.1016/j.placenta.2013.05.011

Cited by 24 publications

(31 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By contrast, labyrinthine markers were significantly reduced, including Gcm1, a gene described as the master regulator of labyrinthine/syncytiotrophoblast differentiation (29). Conversely, our knockdown experiments showed that even a 50% reduction in Sox21 levels led to maintenance of labyrinthine progenitor markers, Ly6e (25) and Epcam (24), induced the expression of labyrinthine trophoblast markers Gcm1 and Muc1, and reduced spongiotrophoblast/TGC markers. These results are consistent with the in vivo localization of some Sox21 mRNA to the ectoplacental cone/early TGCs and its complete absence in the labyrinthine layer.…”

Section: Discussioncontrasting

confidence: 52%

See 1 more Smart Citation

Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation

Zita

Soncin

Natale

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Transcriptional networks in mouse trophoblast stem cells differentiation are not fully characterized. Results: Changes in Sox21 gene expression modulate lineage-specific markers during trophoblast stem cell differentiation. Conclusion:The expression levels of Sox21 need to be tightly regulated to achieve the right balance of terminally differentiated trophoblast cells. Significance: This is the first study showing expression and function of Sox21 in mouse placenta and trophoblast stem cells.

show abstract

Section: Discussioncontrasting

confidence: 52%

“…3, E and F). Moreover, we analyzed the expression of two labyrinthine trophoblast progenitor markers, Epcam and Ly6e (24,25), and found that both were maintained in differentiating Sox21-KD cells (Fig. 4G).…”

Section: Journal Of Biological Chemistrymentioning

confidence: 99%

Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation

Zita

Soncin

Natale

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Analysis of synA, a marker of differentiated labyrinthine syncytiotrophoblast cells, showed no change in expression in control shRNA or AMPKa1/2 shRNA knockdown cells (Fig. 5) [33]. Other nonlabyrinthine lineage markers; Prl3b1 (Pl2), Tpbpa, Ctsq, and Proliferin (Plf ) were not detectable in control shRNA or TGF-b-treated AMPKa1/2 knockdown cells (data not shown).…”

Section: Analysis Of Trophoblast Mrna Expressionmentioning

confidence: 88%

“…Quantitative PCR mRNA expression was assessed by quantitative PCR (q-PCR) using the SYBR green method as previously described [32,33]. One microgram of total RNA was reverse transcribed using the Quantitect Reverse Transcription Kit for SYBR green q-PCR (Qiagen; No.…”

Section: Reverse Transcription-polymerase Chain Reactionmentioning

confidence: 99%

AMPK Knockdown in Placental Trophoblast Cells Results in Altered Morphology and Function

Carey

Albers

Doliboa

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

The placenta is a transient organ that develops upon the initiation of pregnancy and is essential for embryonic development and fetal survival. The rodent placenta consists of distinct lineages and includes cell types that are analogous to those that make up the human placenta. Trophoblast cells within the labyrinth layer, which lies closest to the fetus, fuse and come in contact with maternal blood, thus facilitating nutrient and waste exchange between the mother and the baby. Abnormalities of the placenta may occur as a result of cellular stress and have been associated with pregnancy-associated disorders: such as preeclampsia, intrauterine growth restriction, and placental insufficiency. Cellular stress has also been shown to alter proliferation and differentiation rates of trophoblast cells. This stress response is important for cell survival and ensures continued placental functionality. AMP-activated protein kinase is an important sensor of cellular metabolism and stress. To study the role of AMPK in the trophoblast cells, we used RNA interference to simultaneously knockdown levels of both the AMPK alpha isoforms, AMPKa1 and AMPKa2. SM10 trophoblast progenitor cells were transduced with AMPKa1/2 shRNA and stable clones were established to analyze the effects of AMPK knockdown on important cellular functions. Our results indicate that a reduction in AMPK levels causes alterations in cell morphology, growth rate, and nutrient transport, thus identifying an important role for AMPK in the regulation of placental trophoblast differentiation.

show abstract

“…Resulting placentas and corresponding embryos were assessed for GFP expression by epifluorescence. Tissue sections (6μM) were processed and analyzed by epifluorescence or in situ hybridization with a cRNA probe to detect and confirm GFP mRNA expression [21-23]. …”

Section: Methodsmentioning

confidence: 99%

Important aspects of placental-specific gene transfer

et al. 2014

View full text Add to dashboard Cite

The placenta is a unique and highly complex organ that develops only during pregnancy and is essential for growth and survival of the developing fetus. The placenta provides the vital exchange of gases and wastes, the necessary nutrients for fetal development, acts as immune barrier that protects against maternal rejection, and produces numerous hormones and growth factors that promote fetal maturity to regulate pregnancy until parturition. Abnormal placental development is a major underlying cause of Pregnancy-Associated Disorders that often result in preterm birth. Defects in placental stem cell propagation, growth, and differentiation are major factors that affect embryonic and fetal well being and dramatically increase the risk of pregnancy complications. Understanding the processes that regulate placentation is important in determining the underlying factors behind abnormal placental development. The ability to manipulate genes in a placenta-specific manner provides a unique tool to analyze development and eliminates potentially confounding results that can occur with traditional gene knockouts. Trophoblast stem cells and mouse embryos are not overly amenable to traditional gene transfer techniques. Most viral vectors, however; have a low infection rate and often lead to mosaic transgenesis. While the traditional method of embryo transfer is intrauterine surgical implantation, the methodology reported here, combining lentiviral blastocyst infection and nonsurgical embryo transfer, leads to highly efficient and placental-specific gene transfer. Numerous advantages of our optimized procedures include increased investigator safety, a reduction in animal stress, rapid and noninvasive embryo transfer, and higher a rate of pregnancy and live birth.

show abstract

Ly6e expression is restricted to syncytiotrophoblast cells of the mouse placenta

Cited by 24 publications

References 16 publications

Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation

Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation

AMPK Knockdown in Placental Trophoblast Cells Results in Altered Morphology and Function

Important aspects of placental-specific gene transfer

Contact Info

Product

Resources

About