Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression

Pflugh, David L.; Maher, Stephen E.; Bothwell, Alfred L.M.

doi:10.4049/jimmunol.165.1.313

Cited by 20 publications

(17 citation statements)

References 44 publications

(32 reference statements)

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“…Although the role of Ly6i is unknown, it has been proposed as a maturation marker for T and B lymphocytes as well as for subsets of monocytes and granulocytes (44). The Immunoresponsive gene1 (Irg1) was highly up-regulated as well.…”

Section: Global Changes In the Transcriptional Response During The Chmentioning

confidence: 83%

Immune Response toMycobacterium tuberculosisand Identification of Molecular Markers of Disease

Gonzalez-Juarrero

Kingry²,

Ordway³

et al. 2009

Am J Respir Cell Mol Biol

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Section: Global Changes In the Transcriptional Response During The Chmentioning

confidence: 83%

Immune Response toMycobacterium tuberculosisand Identification of Molecular Markers of Disease

Gonzalez-Juarrero

Kingry²,

Ordway³

et al. 2009

Am J Respir Cell Mol Biol

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“…CD11b is expressed on macrophages, granulocytes, NK cells, and dendritic cells. Sca-1 (Ly-6A/E) is constitutively expressed on hematopoietic stem cells and is also upregulated in peripheral lymphocytes and monocytes upon activation in BALB/c mice (27,33). One of the most studied aspects of the Ly-6 family of proteins, and particularly Ly-6A/E, is their exquisite sensitivity to induction by IFN-␣/␤ and IFN-␥ (20,39).…”

Section: Discussionmentioning

confidence: 99%

“…CD11b is widely expressed on myeloid lineage cells, including macrophages and neutrophils, as well as on NK cells (15,24). Sca-1 is expressed on all hematopoietic stem cells and is also an activation marker of diverse types of lymphoid and myeloid lineage cells (23,31,33,40). In PBS-treated mice, 6.6% of splenocytes were CD11b ϩ / Sca-1 Ϫ (Fig.…”

Section: Cytokine Expression Bymentioning

confidence: 99%

Influence of Vector-Encoded Cytokines on Anti-SalmonellaImmunity: Divergent Effects of Interleukin-2 and Tumor Necrosis Factor Alpha

et al. 2001

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Attenuated Salmonella strains are of interest as new vaccine candidates and as vectors of cloned genes of other organisms. Attenuated strains expressing specific cytokines were constructed as a means of manipulating the immune response in various disease settings. In the present study, interleukin-2 (IL-2)-expressing (GIDIL2) or tumor necrosis factor alpha (TNF-␣)-expressing (GIDTNF) strains were compared with the parent strain (BRD509) for the effect of cytokines on anti-Salmonella immunity. Expression of IL-2 resulted in a rapid clearance of the organism soon after vaccination. The reduction in GIDIL2 CFU was 50-to 300-fold higher than that of BRD509 and correlated with a markedly decreased splenomegaly. Furthermore, no evidence for any significant activation, including upregulation of surface markers and production of nitric oxide (NO), was observed in spleens of GIDIL2-injected mice. In contrast, the host response to GIDTNF was marked by an early, strong, splenic cellular influx, but surprisingly, the degree of induced splenomegaly and NO secretion was only 50% of that observed in BRD509-treated mice. Despite this, bacterial colonization of the spleen in GIDTNF-immunized animals was either slightly decreased from or equivalent to that of the BRD509-treated group, suggesting the induction of additional antimicrobial mechanisms by TNF-␣. In vivo protection studies demonstrated that, at limiting doses, GIDIL2 was inferior to GIDTNF and BRD509 in its capacity to protect against virulent challenge. At high doses, however, all three strains exhibited equal protective efficacy. These results demonstrate that the immune response against intracellular bacteria can be manipulated by pathogenexpressed cytokines and open the way for further fine tuning of immune responses not only to Salmonella strains themselves but also to the heterologous gene(s) carried by them.

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“…Cell lines were maintained as previously described (11). CH27 and COS-7 cells were cultured in IMDM.…”

Section: Cell Linesmentioning

confidence: 99%

“…Ly-6A and Ly-6E were recognized to be alleles of the same gene, and the Ly-6A/E protein is readily detected by the Sca-1 mAb and is perhaps the most useful marker in the isolation of the long term reconstituting hemopoietic stem cell (9,10). Recently, the gene for Ly-6I was characterized (11) and was found to be near the genes for both Ly-6A/E and TSA-1/Sca-2/Ly-6e on mouse chromosome 15 (12,13).…”

mentioning

confidence: 99%

Ly-6 Superfamily Members Ly-6A/E, Ly-6C, and Ly-6I Recognize Two Potential Ligands Expressed by B Lymphocytes

Pflugh

Maher

Bothwell

2002

The Journal of Immunology

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Most hemopoietic cells express one or more members of the Ly-6 supergene family of small glycosylphosphatidylinositol-linked proteins. Although levels of Ly-6 proteins vary with stages of differentiation and activation, their function largely remains unknown. To ascertain whether ligands for Ly-6 proteins exist, chimeric proteins were constructed in which Ly-6E, Ly-6C, and Ly-6I were fused to the murine IgM heavy chain. These chimeras specifically stained both developing and mature B lymphocytes, as assessed by flow cytometry. Analysis of variants of the CH27 B cell lymphoma revealed that Ly-6A/E and Ly-6I recognized different molecules. CH27 cells with low levels of Ly-6A/E ligand activity also lost expression of CD22, and cells transfected with CD22 gained the ability to bind the Ly-6A/E chimera and, to a lesser extent, the Ly-6C and Ly-6I chimeric proteins. As many mature B cells coexpress Ly-6A/E and CD22, the function of Ly-6 molecules may be to associate with other membrane proteins, possibly concentrating these ligands in lipid rafts, rather than acting directly as cell:cell adhesion molecules.

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Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression

Cited by 20 publications

References 44 publications

Immune Response toMycobacterium tuberculosisand Identification of Molecular Markers of Disease

Immune Response toMycobacterium tuberculosisand Identification of Molecular Markers of Disease

Influence of Vector-Encoded Cytokines on Anti-SalmonellaImmunity: Divergent Effects of Interleukin-2 and Tumor Necrosis Factor Alpha

Ly-6 Superfamily Members Ly-6A/E, Ly-6C, and Ly-6I Recognize Two Potential Ligands Expressed by B Lymphocytes

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