LuxS-mediated quorum sensing system in Lactobacillus plantarum NMD-17 from koumiss: induction of plantaricin MX in co-cultivation with certain lactic acid bacteria

Man, Lili; Xiang, Dian-Jun

doi:10.1007/s12223-021-00890-0

Cited by 9 publications

(8 citation statements)

References 41 publications

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“…Whereas the presence of a zone of growth inhibition after 24 h of incubation may be due to the production of metabolites with selective antagonistic activity inherent in not all LAB strains. It is known that the production of bacteriocins can be constitutive or inducible (Maldonado et al 2004;Rizzello et al 2014;Man and Xiang 2021), which may also determine the presence or absence of selective action against indicator strains when using the deferred antagonism method. So, in the previous experiment, the LAB strains were grown for 24 h before indicator strains were streaked.…”

Section: Resultsmentioning

confidence: 99%

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A comparative study of antagonistic activity spectra of lactic acid bacteria isolated from fermented foods

Garmasheva

Oleschenko

2022

Letters in Applied Microbiology

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The present work aimed to assess the general antagonistic activity against opportunistic pathogens and to compare antagonistic action spectra of lactic acid bacteria (LAB) strains, isolated from Ukrainian traditional fermented foods. Overall, 161 profiles of the antagonistic activity spectrum were obtained from 1056 LAB strains. Among them, 114 profiles were genus‐specific and 47 spectra of antagonistic activity were found in LAB strains of different genera. Furthermore, 19 LAB strains were active only against Gram‐negative indicator strains and 149 LAB strains only against Gram‐positive indicator strains. The size of growth inhibition zones of indicator strains by LAB strains of each genus did not correlate with the level of acidification. Zones of growth inhibition of indicator strains appeared after 6–8 h of incubation and in most cases decreased with further incubation, up to absence after 24 h. The difference in the antagonistic activity of 16‐h‐old and 24‐h‐old hours LAB cultures also was found. Among LAB tested, 241 strains are the most promising for further practical use, they have antagonistic action towards 10 indicator strains. The cross‐streaking method can be used for rapid screening of bacteriocinogenic LAB strains and has advantages over the well‐diffusion assay. To the best of our knowledge, this is the first report on a comparative characteristic of spectra of antagonistic activity against opportunistic pathogens of LAB strains belonging to different genera.

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Section: Resultsmentioning

confidence: 99%

“…2004; Rizzello et al . 2014; Man and Xiang 2021), which may also determine the presence or absence of selective action against indicator strains when using the deferred antagonism method.…”

Section: Resultsmentioning

confidence: 99%

A comparative study of antagonistic activity spectra of lactic acid bacteria isolated from fermented foods

Garmasheva

Oleschenko

2022

Letters in Applied Microbiology

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“…As observed for other bacteriocins, the production of plantaricins is regulated through a quorum-sensing based network and seems to be switched on by specific signals that characterise the natural niche of the strain [ 37 ]. Stress conditions and co-culture with other specific inducing bacteria usually enhance plantaricin gene expression [ 38 , 39 , 40 ]. Likewise, the accumulation of signalling molecules, such as the PlnA peptide, outside the bacterial cells, can modulate bacteriocin production [ 40 ].…”

Section: Nature and Mechanisms Of L Plantarum Antimicrobialsmentioning

confidence: 99%

Bioprospecting Antimicrobials from Lactiplantibacillus plantarum: Key Factors Underlying Its Probiotic Action

Rocchetti

Russo

Capozzi

et al. 2021

IJMS

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Lactiplantibacillus plantarum (L. plantarum) is a well-studied and versatile species of lactobacilli. It is found in several niches, including human mucosal surfaces, and it is largely employed in the food industry and boasts a millenary tradition of safe use, sharing a long-lasting relationship with humans. L. plantarum is generally recognised as safe and exhibits a strong probiotic character, so that several strains are commercialised as health-promoting supplements and functional food products. For these reasons, L. plantarum represents a valuable model to gain insight into the nature and mechanisms of antimicrobials as key factors underlying the probiotic action of health-promoting microbes. Probiotic antimicrobials can inhibit the growth of pathogens in the gut ensuring the intestinal homeostasis and contributing to the host health. Furthermore, they may be attractive alternatives to conventional antibiotics, holding potential in several biomedical applications. The aim of this review is to investigate the most relevant papers published in the last ten years, bioprospecting the antimicrobial activity of characterised probiotic L. plantarum strains. Specifically, it focuses on the different chemical nature, the action spectra and the mechanisms underlying the bioactivity of their antibacterial and antiviral agents. Emerging trends in postbiotics, some in vivo applications of L. plantarum antimicrobials, including strengths and limitations of their therapeutic potential, are addressed and discussed.

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“…Several strategies were used to enhance plantaricin biosynthesis, including the screening for high-yield strains, optimizing their production conditions, physical and chemical mutagenesis, and heterologous expression. Nevertheless, they have some drawbacks, including inadequate specificity, restricted growth potential, genetic instability, and low safety (Man and Xiang, 2021). Recent research indicates that microbial co-culture fermentations are favorable for bacteriocin production and are extensively used among L. plantarum strains that produce bacteriocin (Maldonado-Barragán et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis BS-15 Effectively Improves Plantaricin Production and the Regulatory Biosynthesis in Lactiplantibacillus plantarum RX-8

et al. 2022

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Plantaricin is a broad-spectrum bacteriocin produced by Lactiplantibacillus plantarum with significant food industry application potential. It was found that the plantaricin production of L. plantarum RX-8 was enhanced when co-culturing with Bacillus subtilis BS-15. This study, therefore, set out to explore how B. subtilis BS-15 induces biosynthesis of plantaricin. The effect of co-culturing with B. subtilis BS-15 on cell growth, plantaricin production, quorum-sensing (QS) signal molecule PlnA/autoinducer-2 (AI-2) secretion, as well as plantaricin biosynthesis gene cluster and AI-2 synthesis-associated gene expression, was investigated in bacteriocin-producer L. plantarum RX-8. When L. plantarum RX-8 and B. subtilis BS-15 were co-inoculated in Man–Rogosa–Sharp (MRS) for 20 h at an inoculum ratio of 1:1 (106:106 CFU/ml), the greatest plantaricin output (2,048 AU/ml) was obtained, rising by 32-fold compared with the monoculture of L. plantarum RX-8. Additionally, co-culture increased PlnA-inducing activity and AI-2 activity by 8- and 1.14-fold, respectively, over monoculture. RT-qPCR findings generated every 4 h (4–32 h) demonstrated that B. subtilis BS-15 remarkably improved the transcription of plnABCD and plnEF, and increased pfs and luxS transcription, even when using 200 mM D-ribose, a kind of AI-2 inhibitor. Based on the above findings, co-culturing with B. subtilis BS-15 as an environmental stimulus could activate the plantaricin induction via the PlnA-mediated intraspecies QS system and the AI-2-mediated interspecies QS system. Moreover, the inducing effect of PlnA and AI-2 in co-culture was independent. Differential proteomics analysis of B. subtilis BS-15 in co-culture indicated that bacteriocin-inducing regulatory mechanism may be related to flagellar assembly, peptidoglycan biosynthesis, anaerobic respiration, glycine cleavage system, or thiamin pyrophosphate biosynthesis.

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LuxS-mediated quorum sensing system in Lactobacillus plantarum NMD-17 from koumiss: induction of plantaricin MX in co-cultivation with certain lactic acid bacteria

Cited by 9 publications

References 41 publications

A comparative study of antagonistic activity spectra of lactic acid bacteria isolated from fermented foods

A comparative study of antagonistic activity spectra of lactic acid bacteria isolated from fermented foods

Bioprospecting Antimicrobials from Lactiplantibacillus plantarum: Key Factors Underlying Its Probiotic Action

Bacillus subtilis BS-15 Effectively Improves Plantaricin Production and the Regulatory Biosynthesis in Lactiplantibacillus plantarum RX-8

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