Lutibacter oricola sp. nov., a marine bacterium isolated from seawater

Abstract

A bacterial strain, UDC377 T , was isolated from seawater samples collected at Seo-do on the coast of Dokdo island in the East Sea, and was subjected to taxonomic study using a polyphasic approach. Strain UDC377 T was pale-yellow, Gram-staining-negative, non-motile, rod-shaped and aerobic. The strain grew optimally at 25-28 6C, in the presence of 2 % (w/v) NaCl and at pH 7.0-7.5. Strain UDC377 T produced carotenoid pigments; however, it did not produce flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UDC377 T clustered with members of the genus Lutibacter and appeared most closely related to Lutibacter agarilyticus KYW566 T (96.0 % 16S rRNA gene sequence similarity) followed by L. aestuarii MA-My1 T (95.0 %), L. litoralis CL-TF09 T (94.9 %), L. maritimus S7-2 T (94.1 %) and L. flavus IMCC1507 T (94.0 %). The DNA G+C content of strain UDC377 T was 30.8 mol%. Strain UDC377 T contained MK-6 as the predominant menaquinone, iso-C 15 : 0 3-OH, iso-C 15 : 0 and iso-C 16 : 0 3-OH as the major fatty acids, and phosphatidylethanolamine, two unknown aminolipids and six unknown lipids as the major polar lipids. Based on phenotypic properties and phylogenetic data presented, strain UDC377 T is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter oricola sp. nov. is proposed. The type strain is UDC377 T (5DSM 24956 T 5KCTC 23668 T ).The genus Lutibacter in the family Flavobacteriaceae (Jooste, 1985) of the phylum Bacteroidetes (Garrity & Holt, 2001) was first proposed by Choi & Cho (2006) and, at the time of writing, comprises five species with validly published names. These five species, Lutibacter aestuarii (Lee et al., 2012), , 2006) and L. maritimus (Park et al., 2010), were all isolated from the western and southern coasts of the Korean peninsula. Species of the genus Lutibacter are Gram-staining-negative, aerobic, rod-shaped and yellowpigmented bacteria that contain menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine (PE) and several unidentified lipids as the major polar lipids. During investigation of the biodiversity of marine bacteria from seawater, a novel pale-yellow bacterium was isolated. In the present study, we investigated the taxonomic position of this strain, designated UDC377 T , using a polyphasic approach that included morphological, physiological, biochemical and chemotaxonomic characterization, as well as phylogenetic analysis.In April 2008, strain UDC377 T was isolated from a sample of seawater collected at on the coast of Dokdo island, in the East Sea. The strain was isolated on marine agar 2216 (MA; BD) (Yang et al., 2006), by means of the standard dilution plating technique and incubation at 25 u C for 7 days. The isolate was routinely incubated on MA, and preserved at 270 u C in marine broth (MB; BD) containing 15 % (v/v) glycerol. Cell morphology was investigated by light microscopy (Sw 804255; Samwon) and transmission electron microscopy (H-7100; Hitachi), using cells tha...

Complete genome sequence of Lutibacter profundi LP1T isolated from an Arctic deep-sea hydrothermal vent system

“…The genus Lutibacter , including L. profundi LP1 T , thus far comprise nine strains which are proposed to represent novel species: L. litoralis CL-TF09 T [25], L. maritimus S7-2 T [26], L. aestuarii MA-My1 T [27], L. flavus T [29] , L. agarilyticus KYW566 T [28], L. oricola UDC377 T [3], L. crassostreae TYO-8 T [30] and L. holmesii KMM 6277 T [31]. The strain L. crassostreae TYO-8 T was isolated from an oyster collected from the South Sea, South Korea [30], whereas L. holmesii KMM 6277 T was isolated from an sea urchin collected from Troitas Bay, Sea of Japan [31].…”

“…Members of this family are abundant in marine and freshwater habitats and have been isolated from seawater [2, 3], sea ice [4], fresh water [5], glaciers [6, 7], marine plants and animals [4, 8]. In addition, metagenomic studies have shown the presence of Bacteroidetes in marine sediments [9, 10].…”

“…Until now, all published strains have been isolated from Korean coastal waters or the Sea of Japan, and found either as free-living or in association with invertebrates [3, 25–31]. In contrast, L. profundi LP1 T was isolated from a biofilm attached to the outer surface of a black smoker chimney from the LCVF located at the AMOR [1].…”

Lutibacter profundi sp. nov., isolated from a deep-sea hydrothermal system on the Arctic Mid-Ocean Ridge and emended description of the genus Lutibacter

“…In 2006, the genus Lutibacter was proposed along with the isolation and characterization of Lutibacter litoralis (Choi & Cho, 2006). Since then, seven other species have been characterized and the names validly published: Lutibacter maritimus (Park et al, 2010), Lutibacter aestuarii , Lutibacter flavus (Choi et al, 2013), Lutibacter agarilyticus (Park et al, 2013), Lutibacter oricola (Sung et al, 2015), Lutibacter crassostreae (Park et al, 2015), and Lutibacter holmesii (Nedashkovskaya et al, 2015). So far, all species of the genus Lutibacter have been isolated from a tidal area or shallow coastal waters in South Korea and the Sea of Japan.…”

Taeania maliponensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater

“…Currently, the family Flavobacteriaceae comprises more than 110 genera (http://www.bacterio.net). Recently, many members of the family have been isolated from diverse marine environments, including sediments (Jeong et al, 2013;Jung et al, 2015;Zhang et al, 2015), seawater Gao et al, 2015;Sung et al, 2015) and marine algae (Kim et al, 2012a). In this study, a novel bacterial strain, designated MM-8 T , which was isolated from seawater of Taean in the Korean peninsula, is described.…”