Luteolin alleviates bronchoconstriction and airway hyperreactivity in ovalbumin sensitized mice

Das, Moumita; Ram, Arjun; Ghosh, Balaram

doi:10.1007/s000110300021

Cited by 86 publications

(28 citation statements)

References 12 publications

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“…Luteolin has been predicted to exert its anti-asthmatic effects by acting as an anti-inflammatory drug25373839. However, we found that luteolin strongly inhibited GABA A R-mediated responses in lung epithelia cells.…”

Section: Discussioncontrasting

confidence: 51%

Luteolin Attenuates Airway Mucus Overproduction via Inhibition of the GABAergic System

Shen

Wang

Lin

et al. 2016

Sci Rep

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Airway mucus overproduction is one of the most common symptoms of asthma that causes severe clinical outcomes in patients. Despite the effectiveness of general asthma therapies, specific treatments that prevent mucus overproduction in asthma patients remain lacking. Recent studies have found that activation of GABAA receptors (GABAAR) is important for promoting mucus oversecretion in lung airway epithelia. Here, we report that luteolin, a natural flavonoid compound, suppresses mucus overproduction by functionally inhibiting the GABAergic system. This hypothesis was investigated by testing the effects of luteolin on goblet cell hyperplasia, excessive mucus secretion, and GABAergic transmission using histological and electrophysiological approaches. Our results showed that 10 mg/kg luteolin significantly decreased the number of goblet cells in the lung tissue and inhibited mucus overproduction in an in vivo asthma model induced by ovalbumin (OVA) in mice. Patch-clamp recordings showed that luteolin inhibited GABAAR-mediated currents in A549 cells. Furthermore, the inhibitory effects of luteolin on OVA-induced goblet cell hyperplasia and mucus overproduction were occluded by the GABAAR antagonist picrotoxin. In conclusion, our observations indicate that luteolin effectively attenuates mucus overproduction at least partially by inhibiting GABAARs, suggesting the potential for therapeutic administration of luteolin in the treatment of mucus overproduction in asthma patients.

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Section: Discussioncontrasting

confidence: 51%

Luteolin Attenuates Airway Mucus Overproduction via Inhibition of the GABAergic System

Shen

Wang

Lin

et al. 2016

Sci Rep

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“…The cytokines IL-4 and IL-5 were measured in BAL fluid supernatant and OVA-specific IgE levels in sera were measured by enzyme-linked immunosorbent assay (ELISA), as described previously [14, 19]. Corticosterone in the BAL fluid was assessed using an ELISA kit obtained from Neogen (USA).…”

Section: Methodsmentioning

confidence: 99%

“…There is an intense need to identify new molecules preferably from natural origin with negligible or no adverse effects for treating this epidemic disease. In view of this, several herbal compounds such as curcumin, luteolin, glycyrrhizin, verprosides and cannabinoids have been identified as anti-asthmatic agents in animal models of asthma [13,14,15,16,17], and efforts are still continued to search other potent herbal molecules. Based on its anti-inflammatory activity [5, 6], we speculated that CBX may also possess anti-asthmatic activity.…”

Section: Introductionmentioning

confidence: 99%

Inhaled Carbenoxolone Prevents Allergic Airway Inflammation and Airway Hyperreactivity in a Mouse Model of Asthma

Ram

Singh

et al. 2008

Int Arch Allergy Immunol

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Background: Asthma is a chronic respiratory disease, which needs a safer medication preferably in inhalation form. In view of this, we have evaluated the effect of inhaled carbenoxolone (CBX), a herbal-derived compound, on asthma in a mouse model. Methods: Mice were sensitized and challenged with ovalbumin (OVA) to develop certain characteristic features of asthma such as airway hyperreactivity (AHR), airway eosinophilia, lung inflammation and mucus hypersecretion. To evaluate the effect of CBX on the above asthmatic features, CBX (2.5, 5 and 10 mg/ml, 3 ml) or vehicle (water) was given by inhalation. AHR was determined using whole-body plethysmography. Infiltration of eosinophils was estimated by microscopy. Lung inflammation and mucus hypersecretion were assessed using hematoxylin and eosin, and periodic acid-Schiff staining, respectively. Th-2 cytokines, IL-4 and IL-5 were measured in bronchoalveolar lavage (BAL) fluid and IgE in sera. To identify the possible mode of CBX action, we measured corticosterone levels in the BAL fluid and 5-lipoxygenase (5-LO) expression in the lungs. Results: CBX (5 mg/ml) inhalation markedly alleviated AHR (p = 0.0032) and reduced lung inflammation and mucus hypersecretion. Also, it prevented the increase in IL-4 (p = 0.0192), IL-5 (p = 0.0116) and eosinophils (p < 0.0005) in the BAL fluid, and OVA-specific IgE levels (p = 0.00061) in sera. 5-LO expression was also markedly reduced. However, corticosterone levels were not affected. Conclusions: Inhaled CBX alleviates the asthmatic features in mice and could be a potent nebulized therapy in clinical asthma.

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“…Furthermore, an epidemiologic study found that a higher intake of flavonoids was associated with a lower incidence of asthma [44]. In experimental models, oral administration of flavonoids has been demonstrated to prevent allergic diseases including asthma, anaphylaxis, food allergies and atopic dermatitis [16, 17,32,33,34,35,36,37]. This study showed that the ocular symptoms were reduced in the EMIQ group.…”

Section: Discussionmentioning

confidence: 71%

“…We and others have shown that some kinds of flavonoids including apigenin, fisetin, luteolin and quercetin suppress the expression of Th2-type (IL-4, IL-5 and IL-13) [13, 14], proinflammatory cytokines [31] and CD40 ligand [15] in basophils or in mast cells. In addition to these in vitro antiallergic activities, studies with allergic animal models have demonstrated that flavonoids exert preventative and therapeutic effects by modifying Th2-type cytokine expression and suppressing IgE elevation [16, 17,32,33,34,35,36,37]. In our study we measured the serum levels of cytokines and chemokines before and after the study but found no significant reduction in Th2 cytokines (IL-4, IL-5 and IL-13), chemokines (eotaxin) or IgE concentration as a result of EMIQ intake except for TARC (fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of Enzymatically Modified Isoquercitrin, a Flavonoid, on Symptoms of Japanese Cedar Pollinosis: A Randomized Double-Blind Placebo-Controlled Trial

Kawai

Hirano

Arimitsu

et al. 2009

Int Arch Allergy Immunol

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Background: Flavonoids exert antiallergic and antioxidant effects. We investigated the efficacy of enzymatically modified isoquercitrin (EMIQ), a flavonoid, to relieve symptoms of pollinosis. Methods: In a parallel-group, double-blind placebo-controlled study design, 20 subjects with Japanese cedar pollinosis took two capsules daily of 100 mg EMIQ or a placebo for 8 weeks during the pollen season. Subjective symptoms and activities of daily living (ADL) scores were recorded every day, and the quality of life (QOL) score was obtained every 4 weeks. Blood sampling was performed before and after the study to measure serum cytokines, chemokines, IgE, quercetin and oxidized biomarkers. Results: During the entire study period, total ocular score and ocular itching score for the EMIQ group were significantly lower (p < 0.05) than for the placebo group. When limited to the individual periods, total symptom score for the EMIQ group was significantly lower (p < 0.05, week 4–5) than that for the placebo group while other scores for the EMIQ group, such as total nasal score (p = 0.06, week 4–5), nasal obstruction score (p = 0.08, week 4–5), lacrimation score (p = 0.06, week 5–6), ocular congestion score (p = 0.08, week 4–7) and ADL score (p = 0.08, week 4–7), all tended to be lower. The levels of serum cytokines such as interleukin (IL)-4, IL-5, IL-12, IL-13, interferon-γ, and eotaxin and IgE were not significantly downregulated by the intake of EMIQ but the serum concentrations of oxidized low-density lipoprotein and thymus and activation-regulated chemokine were reduced. Conclusion: Intake of the quercetin glycoside EMIQ was safe and influenced ocular symptoms caused by pollinosis.

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Luteolin alleviates bronchoconstriction and airway hyperreactivity in ovalbumin sensitized mice

Cited by 86 publications

References 12 publications

Luteolin Attenuates Airway Mucus Overproduction via Inhibition of the GABAergic System

Luteolin Attenuates Airway Mucus Overproduction via Inhibition of the GABAergic System

Inhaled Carbenoxolone Prevents Allergic Airway Inflammation and Airway Hyperreactivity in a Mouse Model of Asthma

Effect of Enzymatically Modified Isoquercitrin, a Flavonoid, on Symptoms of Japanese Cedar Pollinosis: A Randomized Double-Blind Placebo-Controlled Trial

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