Luteolibacter flavescens sp. nov., isolated from deep seawater

Abstract

A novel Gram-stain-negative, rod-shaped, non-motile strain, designated GKXT, was isolated from deep seawater. Strain GKXT was able to grow at 20-35 °C (optimum, 25 °C), pH 5.5-9.5 (optimum, 7.5) and 0-4.0 % (w/v) NaCl (optimum, 1.0 %). The major fatty acids were C16 : 1ω9c (15.4 %), C16 : 0 (18.4 %), C14 : 0 (12.0 %), iso-C14 : 0 (30.1 %) and anteiso-C15 : 0 (5.7 %). Strain GKXT contained phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as the main polar lipids. The only isoprenoid quinone was menaquinone-9. The diagnostic amino acids of the cell-wall peptidoglycan contained meso-diaminopimelic acid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GKXT belonged to the genus Luteolibacter in the family Verrucomicrobiaceae. The 16S rRNA gene sequence of this strain showed 98.0, 93.5 and 93.3 % sequence similarity, respectively, with those of Luteolibacter arcticus MC 3726T, L.uteolibacter pohnpeiensisA4T-83T and L.uteolibacter cuticulihirudinis E100T. DNA-DNA hybridization value of strain GKXT with L. arcticus MC 3726T was 33.1 %. The G+C content of the genomic DNA was 59.5 mol%. On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain GKXT was proposed to represent a novel species of the genus Luteolibacter, named Luteolibacter flavescens sp. nov. (type strain GKXT=MCCC 1K03193T=KCTC 52361T).

Luteolibacter ambystomatis sp. nov., isolated from the skin of an Anderson’s salamander (Ambystoma andersoni)

“…This profile was more complex than that reported for L. cuticulihirudinis E100 T [3, 4] and did not show significant congruence with those of other Luteolibacter species. Polyamines were extracted and analysed as reported previously [26, 27].…”

“…The genus was assigned to the class Verrucomicrobiae , a separate line of descent within the domain Bacteria . Meanwhile, seven additional species have been described, namely Luteolibacter arcticus [2], Luteolibacter cuticulihirudinis [3], Luteolibacter flavescens [4], Luteolibacter gellanilyticus [5], Luteolibacter luojiensis [6], Luteolibacter luteus [7] and Luteolibacter yonseiensis [8]. Species of the genus have been isolated from different sources such as activated sludge, stream bank soil, Arctic tundra soil, soil of a typical Mediterranean forest system, deep seawater, surface of a medicinal leech, soil from a high Arctic tundra, driftwood, and a red alga.…”

Seasonal shifts of microbial methane oxidation in Arctic shelf waters above gas seeps

“…6B). Luteolibacter comprises six known species that are described as chemoheterotrophs utilizing a variety of carbon sources (Zhang et al 2017). None of them has been tested for MOx activity and to the best of our knowledge, no genome data from this genus are available.…”

Genome-Resolved Metagenomics Extends the Environmental Distribution of the Verrucomicrobia Phylum to the Deep Terrestrial Subsurface

“…Verrucomicrobia have been isolated from diverse habitats, including soil (6–12), freshwater (12), marine (13–17), geothermal (18–20), and hypersaline (21) systems. Additionally, members of this phylum are distributed in host-associated biomes, with recovery of Verrucomicrobia genomes from sugar beet roots (22) and the gastrointestinal tracts of termites (23) and humans (24), as well as extreme environments, like cryoconite holes in glaciers (25), the deep (300-m) subsurface (26), and hypersaline lakes (27–29).…”