Lutein enhances survival and reduces neuronal damage in a mouse model of ischemic stroke

Li, Suk-Yee; Yang, Di; Fu, Zhongjie; Woo, Tiffany T. Y.; Wong, David; Lo, Amy Cheuk Yin

doi:10.1016/j.nbd.2011.10.008

Cited by 72 publications

(44 citation statements)

References 51 publications

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“…Also the lutein adjuvant therapies need further studies to improve effective drug molecules. Lutein could diminish the deleterious outcomes of cerebral I/R in stroke patients [29]. The present study was supported by this information which explains the inhibitory action of aldose reductase by lutein and zeaxanthin.…”

Section: экспериментальная диабетологияsupporting

confidence: 84%

In silico validation of microalgal metabolites against Diabetes mellitus

et al. 2017

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Aim. Present study aimed to evaluate the efficiency of microalgal metabolites as ligands for anti-diabetic target proteins viz., glucokinase, fructose-1, 6-bisphosphatase, glycogen synthase kinase, cytochrome P450, multi drug resistant protein, and peroxisome proliferator-activated receptor-(PPAR) via computational approach. Matherials and methods. Three-dimensional structures of microalgal metabolites were retrieved from PubChem database and were energy minimized. The active site of target protein was predicted through PDB sum. Molecular docking was performed with microalgae metabolites by using Hex 8.0 and DockThor server. Results. Hex docking revealed that the binding interaction of fucoxanthin was higher with fructose 1.6 bis-phosphatase (-298.31), human multidrug resistant protein 1 (-369.67), and PPAR(-404.18). DockThor docking indicated that zeaxanthin with glucokinase produced higher total energy (111.23 kcal/mol) and interaction energy (-2.99 kcal/mol). Lutein with fructose 1.6 bis phosphatase, human multidrug resistant protein, glycogen synthase kinase, PPARand cytochrome p450 produced higher total energy and interaction energy. Conclusion. Further studies will assess the anti-diabetic effect of carotenoids of microalgae especially lutein, zeaxanthin and fucoxanthin.

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Section: экспериментальная диабетологияsupporting

confidence: 84%

In silico validation of microalgal metabolites against Diabetes mellitus

et al. 2017

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“…Sun et al 1088 lutein (0.2 mg/kg) given to mice intraperitoneally (i.p.) 1 h after middle cerebral artery occlusion and 1 h after reperfusion could protect the brain from ischemia/ reperfusion injury by its anti-apoptotic and anti-inflammatory properties at 7 days after reperfusion [9]. In the present study, we found that lutein could prevent nerve injury by reducing oxidative stress.…”

Section: Discussionsupporting

confidence: 60%

Treatment with lutein provides neuroprotection in mice subjected to transient cerebral ischemia

Sun

Liu

Dai

et al. 2014

Journal of Asian Natural Products Research

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Lutein is known to be a nonprovitamin A carotenoid found in broccoli and spinach. The aim of present study was to investigate whether lutein can protect brain against ischemic injury by reducing oxidative stress. Male ICR mice were randomly divided into five experimental groups: model group, sham group, lutein high, middle, and low-dose groups (30, 15, and 7.5 mg/kg). Mice were subjected to a 2-h middle cerebral artery occlusion followed by reperfusion for 22 h. The reduced glutathione/oxidized glutathione (GSH/GSSG) ratio, antioxidant enzyme activities, malondialdehyde (MDA), and the carbonyl content in oxidatively modified proteins in brain tissue were determined with colorimetric method. The 8-hydroxy deoxyguanosine (8-OHdG) expression was measured by immunohistochemistry assay, and the neuron apoptosis was detected by TdT-mediated dUTP nick end labeling assay. Then, the neurological deficit scores were measured at last. Treatment of lutein significantly elevated the ratio of GSH/GSSG as well as activities of superoxide dismutase, glutathione peroxidase, and catalase and obviously decreased the contents of MDA, brain carbonyl, the expression of 8-OHdG, the number of apoptotic cells, and neurological deficit scores. Our results demonstrate that administration of lutein affords strong neuroprotective effect against transient cerebral ischemic injury and that the effect might be associated with its antioxidant property.

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“…53 Treatment of lutein increased survival rate and decreased cell death by inhibiting the NF-jB signaling in an animal model of ischemic stroke in our previous study. 25 Lutein decreased lipopolysaccharides (LPS)-induced inflammation by modulating gene and protein expression of IL-1b, COX-2, TNFa, and inducible form nitric oxide synthase (iNOS) in mouse macrophage cells. 54,55 In animal models of ocular diseases such as laser-induced choroidal neovascularization 56 and LPSinduced uveitis, 17 lutein treatment suppressed inflammation by inhibiting the activation of NF-jB signaling pathway and subsequent up-regulation of pro-inflammatory molecules.…”

Section: Discussionmentioning

confidence: 99%

“…Semiquantitative analysis was used to assess the immunoreactivity as previously described. 25,36 Briefly, all retinal sections for analysis were processed at the same time in a single round of IHC experiment. After the immunohistochemical procedures, microscopic slides were randomly coded and examined in a blinded approach.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

“…[17][18][19][20][21][22][23] Lutein has also been shown to display anti-inflammatory effects in ocular uveitis, 17 atherosclerosis, 24 and cerebral I/R. 25 We have previously shown that lutein treatment could diminish oxidative stress and apoptosis, therefore, protecting retinal neurons from ischemia/hypoxia in vivo 23 and in vitro. 26 As inflammation is a contributing factor in the pathogenesis of retinal I/R, we hypothesized that lutein may also display antiinflammatory effects in protecting the retinal neurons.…”

mentioning

confidence: 99%

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Anti-Inflammatory Effects of Lutein in Retinal Ischemic/Hypoxic Injury: In Vivo and In Vitro Studies

Fung

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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114

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PURPOSE. Lutein protects retinal neurons by its anti-oxidative and anti-apoptotic properties in ischemia/reperfusion (I/R) injury while its anti-inflammatory effects remain unknown. As Müller cells play a critical role in retinal inflammation, the effect of lutein on Müller cells was investigated in a murine model of I/R injury and a culture model of hypoxic damage.METHODS. Unilateral retinal I/R was induced by a blockade of internal carotid artery using the intraluminal method in mice. Ischemia was maintained for 2 hours followed by 22 hours of reperfusion, during which either lutein (0.2 mg/kg) or vehicle was administered. Flash electroretinogram (flash ERG) and glial fibrillary acidic protein (GFAP) activation were assessed. Lutein's effect on Müller cells was further evaluated in immortalized rat Müller cells (rMC-1) challenged with cobalt chloride-induced hypoxia. Levels of IL-1b, cyclooxygenase-2 (Cox-2), TNFa, and nuclear factor-NF-kappa-B (NF-jB) were examined by Western blot analysis. RESULTS.Lutein treatment minimized deterioration of b-wave/awave ratio and oscillatory potentials as well as inhibited upregulation of GFAP in retinal I/R injury. In cultured Müller cells, lutein treatment increased cell viability and reduced level of nuclear NF-jB, IL-1b, and Cox-2, but not TNFa after hypoxic injury.CONCLUSIONS. Reduced gliosis in I/R retina was observed with lutein treatment, which may contribute to preserved retinal function. Less production of pro-inflammatory factors from Müller cells suggested an anti-inflammatory role of lutein in retinal ischemic/hypoxic injury. Together with our previous studies, our results suggest that lutein protected the retina from ischemic/hypoxic damage by its anti-oxidative, antiapoptotic, and anti-inflammatory properties. (Invest Ophthalmol Vis Sci. 2012;53:5976-5984)

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Lutein enhances survival and reduces neuronal damage in a mouse model of ischemic stroke

Cited by 72 publications

References 51 publications

In silico validation of microalgal metabolites against Diabetes mellitus

In silico validation of microalgal metabolites against Diabetes mellitus

Treatment with lutein provides neuroprotection in mice subjected to transient cerebral ischemia

Anti-Inflammatory Effects of Lutein in Retinal Ischemic/Hypoxic Injury: In Vivo and In Vitro Studies

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