Lutein attenuates oxidative stress and inhibits lipid accumulation in free fatty acids-induced HepG2 cells by activating the AMPK pathway

Cheng, Jing; Liu, Dong; Zhao, Jingjing; Li, Xiang; Yan, Yunhui; Wu, Zijian; Wang, Hao; Wang, Chengtao

doi:10.1016/j.jff.2019.103445

Cited by 26 publications

(18 citation statements)

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“…HepG2 cells were grown in DMEM supplemented with 10% FBS, 0.1 U/L penicillin, and 0.1 g/L streptomycin in a humidified incubator at 37 °C with 5% CO 2 . When the cells reached 70% confluence, they were induced with UA and FFAs (OA:PA, 2:1, following by the method reported previously [Gomez‐Lechon et al., 2007]) or compound C (2 µM, referring to a previous experimental dose [Cheng et al., 2019]) for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

“…The oil red O staining assay can reflect intracellular fat content, which can be detected by a previously described method (Cheng et al., 2019). In brief, the cells (5 × 10 4 /mL) were seeded in 96‐well plates, cultivated for 24 hr, and then incubated with FFAs and UA for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

“…AMPK activity was determined in HepG2 cells as described previously to investigate the relationship between AMPK and lipid metabolism (Cheng et al., 2019). An anti‐AMPK‐panα antibody was purchased from Merck (California, USA.).…”

Section: Methodsmentioning

confidence: 99%

“…Molecular docking was conducted using the software of CDOCKER with Discover Studio 3.5 (Accelrys, California, USA), as previously described, to explore the possible interaction mode of UA and AMPK (Cheng et al., 2019). The crystal of AMPK protein (PDB:4CFH) was retrieved from the RCSB Protein Data Bank, and the structure of UA was obtained using the ChemBio3D software.…”

Section: Methodsmentioning

confidence: 99%

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Ursolic acid alleviates lipid accumulation by activating the AMPK signaling pathway in vivo and in vitro

Cheng

Liu

et al. 2020

Journal of Food Science

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The mechanism underlying the effect of ursolic acid (UA) on lipid metabolism remains unclear. This study aimed to explore the mechanisms of UA in reducing lipid accumulation in free fatty acids‐cultured HepG2 cells and in high‐fat‐diet‐fed C57BL/6J mice. In vivo, UA effectively alleviated liver steatosis and decreased the size of adipocytes in the epididymis. It also significantly decreased the total cholesterol (TC) and triglyceride (TG) contents in the liver and plasma in C57BL/6 mice. In vitro, UA (20 µM) significantly reduced lipid accumulation; the intracellular TC contents decreased from 0.078 ± 0.0047 to 0.049 ± 0.0064 µmol/mg protein, and TG contents from 0.133 ± 0.005 to 0.066 ± 0.0047 µmol/mg protein, in HepG2 cells. Furthermore, UA reduced the mRNA expression related to fat synthesis, enhanced the mRNA expression related to adipose decomposition, and dramatically upregulated the protein expression of P‐AMPK in vivo and in vitro. Of note, these protective effects of UA on a high‐fat environment were blocked by the AMPK inhibitor (compound C) in vitro. In addition, the molecular docking results suggested that UA could be docked to the AMPK protein as an AMPK activator. These results indicated that UA lowered the lipid content probably via activating the AMPK signaling pathway, thereby inhibiting lipid synthesis and promoting fat decomposition. Practical Application Ursolic acid (UA) widely exists in vegetables and fruits. This study highlighted a lipid‐lowing mechanism of UA in HepG2 cells and C57BL/6J mice. The data indicated that UA might be used in lipid‐lowering functional foods.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Ursolic acid alleviates lipid accumulation by activating the AMPK signaling pathway in vivo and in vitro

Cheng

Liu

et al. 2020

Journal of Food Science

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“…Previous studies have shown that dorsomorphin exposure inhibits mammalian AMPK phosphorylation and kinase activity ( Liu et al, 2014 ; Cheng et al, 2019 ). However, when adult schistosomes were exposed to 10 μM dorsomorphin for 24 h, a significant increase in phosphorylation of AMPK α was detected by Western blot ( Figures 3A, B ).…”

Section: Resultsmentioning

confidence: 99%

Schistosome AMPK Is Required for Larval Viability and Regulates Glycogen Metabolism in Adult Parasites

et al. 2021

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On entering the mammalian host, schistosomes transition from a freshwater environment where resources are scarce, to an environment where there is an unlimited supply of glucose, their preferred energy substrate. Adult schistosome glycolytic activity consumes almost five times the parasite’s dry weight in glucose per day to meet the parasite’s energy demands, and the schistosome glycolytic enzymes and mechanisms for glucose uptake that sustain this metabolic activity have previously been identified. However, little is known of the parasite processes that regulate schistosome glucose metabolism. We previously described the Schistosoma mansoni ortholog of 5′ AMP-Activated Protein Kinase (AMPK), which is a central regulator of energy metabolism in eukaryotes, and characterized the developmental regulation of its expression and activity in S. mansoni. Here we sought to explore the function of AMPK in schistosomes and test whether it regulates parasite glycolysis. Adult schistosomes mounted a compensatory response to chemical inhibition of AMPK α, resulting in increased AMPK α protein abundance and activity. RNAi inhibition of AMPK α expression, however, suggests that AMPK α is not required for adult schistosome viability in vitro. Larval schistosomula, on the other hand, are sensitive to chemical AMPK α inhibition, and this correlates with inactivity of the AMPK α gene in this life cycle stage that precludes a compensatory response to AMPK inhibition. While our data indicate that AMPK is not essential in adult schistosomes, our results suggest that AMPK regulates adult worm glycogen stores, influencing both glycogen utilization and synthesis. AMPK may therefore play a role in the ability of adult schistosomes to survive in vivo stressors such as transient glucose deprivation and oxidative stress. These findings suggest that AMPK warrants further investigation as a potential drug target, especially for interventions aimed at preventing establishment of a schistosome infection.

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A Review on Bioactive Compounds, Ethnomedicinal Importance and Pharmacological Activities of Talinum triangulare (Jacq.) Willd

Dinesh,

Kumar

2023

Chemistry & Biodiversity

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Talinum triangulare (Jacq.) Willd. is a traditional leafy vegetable used by tribal communities for ethnomedicinal and ethnoculinary preparations. This article reviews the current knowledge of its multiple uses, including pharmacological activities and nutritional composition. The literature survey shows that it has been traditionally useful in the treatment of several diseases, such as anaemia, diabetes, measles, and ulcers and the preparation of various traditional foods. Analysis of the literature on its phytochemicals shows its richness in bioactive compounds. Further, research also shows that this plant has antidiabetic, antiobesity, antitumor, antiulcer, hepatoprotective, and neuroprotective activities besides anti‐inflammatory and antioxidant properties. Nutrient analysis of the plant reveals the presence of Ca, Zn, Fe, vitamins C and E, dietary fibre and protein in considerable quantities. The results of the pharmacological studies on the antidiabetic, antiulcer and anti‐anaemic activities provide support in favour of its ethnomedicinal uses. The presence of bioactive compounds and pharmacological activities show the usefulness of this plant as a functional food.

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Lutein attenuates oxidative stress and inhibits lipid accumulation in free fatty acids-induced HepG2 cells by activating the AMPK pathway

Cited by 26 publications

References 50 publications

Ursolic acid alleviates lipid accumulation by activating the AMPK signaling pathway in vivo and in vitro

Ursolic acid alleviates lipid accumulation by activating the AMPK signaling pathway in vivo and in vitro

Schistosome AMPK Is Required for Larval Viability and Regulates Glycogen Metabolism in Adult Parasites

A Review on Bioactive Compounds, Ethnomedicinal Importance and Pharmacological Activities of Talinum triangulare (Jacq.) Willd

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