Luteal function after ovulation blockade by intrafollicular injection of indomethacin in the ewe

Murdoch, William J.; Dunn, Thomas

doi:10.1530/jrf.0.0690671

Cited by 49 publications

(20 citation statements)

References 15 publications

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“…(Ginther, 1974), also failed to block ovulation in any of the cows treated. (1979) and Murdoch & Dunn (1983) also noted luteinization of the follicles that had not undergone ovulation after indomethacin treatment. Two previous studies (Shemesh & Hansel, 1975;Shemesh, 1979) showed that bovine follicular tissue synthesized PGs under in-vitro conditions and that synthesis was enhanced by LH.…”

Section: Resultsmentioning

confidence: 88%

“…The ovulatory failure may be confined to the preovulatory follicle because after the LH surge, prostaglandin (PG) synthesis within the preovulatory follicle is critically involved in ovulation (Espey, 1980; Armstrong, 1981) and if PG synthesis does not occur then the preovulatory follicle would not rupture and possibly remain as a cyst. Ovulation has been successfully blocked by indomethacin, an inhibitor of PG synthesis, in the mouse (Lau, Saksena & Chang, 1974), rat , rabbit (Grinwich, Kennedy & Armstrong, 1972), monkey (Wallach, de la Cruz, Hunt, Wright & Stevens, 1975), sow (Ainsworth et al, 1979) and ewe (Murdoch & Dunn, 1983) (Steel & Torrie, 1960).…”

Section: Introductionmentioning

confidence: 99%

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Indomethacin inhibition of ovulation in the cow

Silva¹,

Reeves²

1985

Reproduction

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Summary. Indomethacin or saline was administered via intramuscular, intrauterine or intraovarian routes to dairy cows, within 24 h after standing oestrus was first observed.The incidence of ovulation was determined at slaughter. All of the saline-treated cows (18/18) ovulated. Ovulation was not blocked after intramuscular injection (0/6) or intrauterine infusion (0/6) of indomethacin. In all cows, ovulation was blocked after intraovarian injection (6/6) of indomethacin. These findings add support to the hypothesis that prostaglandins play an essential role in ovulation in the cow as in many other mammalian species.

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Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Indomethacin inhibition of ovulation in the cow

Silva¹,

Reeves²

1985

Reproduction

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“…It has been reported that inhibition of follicle rupture by injection of PGE 2 synthesis inhibitor or indomethacin into preovulatory follicle just before LH surge does not alter luteal function in the following luteal phase of cattle [14], sheep [15], and monkeys [16]. In addition, injection of LH into sheep and rabbit preovulatory follicles induced luteinization and P 4 secretion without ovulation [17].…”

Section: Discussionmentioning

confidence: 99%

Effect of the Dominant Follicle Aspiration before or after Luteinizing Hormone Surge on the Corpus Luteum Formation in the Cow

Hayashi

Matsui

Acosta

et al. 2006

Journal of Reproduction and Development

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Abstract. Luteinizing hormone (LH) surge and follicle rupture act as trigger to start corpus luteum (CL) formation. Thus, we aimed to investigate whether a dominant follicle that has not been exposed to an LH surge can become a functional CL. For this purpose, follicular fluid from the dominant follicles (DF) of cows was aspirated before or after a GnRH-induced LH surge, and subsequent CL formation was observed. Holstein cows were divided into four groups as follows: Luteal phase, a DF was aspirated 7 days after GnRH injection; Pre-LH surge, a DF was aspirated 42 h after PGF2α injection during the mid luteal phase; Post-LH surge, a DF was aspirated 24 h after GnRH injection following PGF2α; and Intact follicle, ovulation was induced by GnRH injection after PGF2α. Observation of morphological changes in the aspirated follicle using color Doppler ultrasonography and blood sampling was performed on Days 0, 3, 6, and 9 (Day 0=follicle aspiration). CL formation following DF aspiration was observed only in the Post-LH surge group. In both the Luteal phase and Pre-LH surge groups, however, none of the cows showed local blood flow at the aspirated site or CL formation. Luteal blood flow area, CL volume, and plasma progesterone concentration in the Post-LH surge group were no different from those in the Intact follicle group. The present results clearly demonstrate that rather than follicle rupture, it is the LH surge that is essential for CL formation in cows. Key words: Corpus luteum formation, Cow, Follicle aspiration, LH surge (J. Reprod. Dev. 52: [129][130][131][132][133][134][135] 2006) t is w ell established that preovulatory luteinizing hormone (LH) surge acts as a trigger f o r t h e s u c c e s s i v e p r o c e s s e s o f f o l l i c u l a r luteinization, ovulation, and corpus luteum (CL) f o r m a t i o n b y i n d u c i n g m o r p h o l o g i c a l , endocrinological, and biochemical changes in the preovulatory follicle [1]. One of the most important events of the ovulatory process is follicle rupture. B r e a k d o w n o f t h e b a s e m e n t m e m b r a n e accompanied by follicle rupture permits invasion of theca cells and thecal microcapillary into the avascular granulosa layer, contributing to structural and functional parts in CL formation [1,2]. Several studies indicate that blocking the endogenous LH surge using GnRH antagonist resulted in delay or suppression of ovulation and CL formation in various species [3][4][5].

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“…The ability of indomethacin (PG synthetase inhibitor) to block ovulation is documented for rats (Tsafriri et al, 1973), rabbits (O'Grady et al, 1972), sheep (Murdoch & Dunn, 1983) and cattle (De Silva & Reeves, 1985). Intrafollicular injection of PGF-2a antiserum blocked luteinizing hormone (LH)-induced ovulations in rabbits (Armstrong et al, 1974).…”

Section: Discussionmentioning

confidence: 99%