Luteal expression of factors involved in the metabolism and sensitivity to oestrogens in the dog during pregnancy and in non‐pregnant cycle

Pereira, Miguel Tavares; Papa, Paula de Carvalho; Reichler, Iris M; Aslan, Selim; Kowalewski, Mariusz P.

doi:10.1111/rda.14032

Cited by 8 publications

(15 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The local production and role of P4 has been addressed earlier and there is a consensus that P4 is the primary hormone that maintains corpus luteum function and consequently, pregnancy ( 2 , 3 ). Regarding E2, it is known that the canine CL produces E2 ( 4 , 5 ), expresses enzymes for E2 activation (STS, steroid sulfatase) and deactivation [SULT1E1, sulphotransferase 1E member 1 ( 6 )], and that the expression of E2 receptors (ERa and ERb, encoded by ESR1 and ESR2 genes, respectively) fluctuates during diestrus in pregnant and non-pregnant CL ( 1 , 6 ). The ratio between ESR2 / ESR1 varies along non-pregnant diestrus, being greater on day 40 post-ovulation (p.o.)…”

Section: Introductionmentioning

confidence: 99%

“…compared to days 10, 20, 30 and 60 p.o. ( 6 ). The gene HSD17B7 , encoding 17b-hydroxylase, the enzyme converting estrone to E2, increases from day 10 to 20 and further to day 40 p.o.…”

Section: Introductionmentioning

confidence: 99%

“…The gene HSD17B7 , encoding 17b-hydroxylase, the enzyme converting estrone to E2, increases from day 10 to 20 and further to day 40 p.o. ( 6 ), whereas CYP19A1 , encoding P450 aromatase, is greater expressed between days 35 and 45 p.o. ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Expression of STS mRNA peaks on day 30 p.o., that of SULT1E1 , on days 50 and 60 p.o. ( 6 ), indicating an established local machinery modulating E2 production and activity, which shows a turning point around day 40 p.o., when early luteal regression starts.…”

Section: Introductionmentioning

confidence: 99%

“…Collectively, canine CL expresses both ESR1 and ESR2 , and the ratio of ESR1 / ESR2 varies throughout diestrus. There is a greater ESR1 expression in early-luteal phase ( 1 ) and greater ESR2 expression in the late-luteal phase ( 6 ). However, the role of E2 and its receptors in regulating canine CL function is still unknown.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum

et al. 2022

Self Cite

View full text Add to dashboard Cite

The canine corpus luteum (CL) is able to synthetise, activate and deactivate 17b-estradiol (E2) and also expresses nuclear estrogen receptors in a time-dependent manner during diestrus. Nevertheless, we are still missing a better comprehension of E2 functions in the canine CL, especially regarding the specific roles of estrogen receptor alpha (ERa) and ERb, encoded by ESR1 and 2, respectively. For that purpose, we analyzed transcriptomic data of canine non-pregnant CL collected on days 10, 20, 30, 40, 50 and 60 of diestrus and searched for differentially expressed genes (DEG) containing predicted transcription factor binding sites (TFBS) for ESR1 or ESR2. Based on biological functions of DEG presenting TFBS, expression of select transcripts and corresponding proteins was assessed. Additionally, luteal cells were collected across specific time points during diestrus and specificity of E2 responses was tested using ERa and/or ERb inhibitors. Bioinformatic analyses revealed 517 DEGs containing TFBS, from which 67 for both receptors. In general, abundance of predicted ESR1 targets was greater in the beginning, while abundance of ESR2 targets was greater in the end of diestrus. ESR1/ESR2 ratio shifted from an increasing to a decreasing pattern from day 30 to 40 post ovulation. Specific receptor inhibition suggested an ERa-mediated positive regulation of CL function at the beginning of diestrus and an ERb-mediated effect contributing to luteal regression. In conclusion, our data points toward a broad spectrum of action of E2 and its nuclear receptors, which can also act as transcription factors for other genes regulating canine CL function.

show abstract

Section: Introductionmentioning

confidence: 99%

“…compared to days 10, 20, 30 and 60 p.o. ( 6 ). The gene HSD17B7 , encoding 17b-hydroxylase, the enzyme converting estrone to E2, increases from day 10 to 20 and further to day 40 p.o.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Characterization of bovine uterine fluid extracellular vesicles proteomic profiles at follicular and luteal phases of the oestrous cycle

et al. 2022

View full text Add to dashboard Cite

Extracellular vesicles (EV) have been identified in uterine fluid (UF), however the bovine UF-EV profile during different phases of the oestrous cycle has not yet been established. Therefore, we compared the UF-EV, and their protein profile at follicular and luteal phases of the oestrous cycle. UF samples were collected from healthy uteri of six live and six slaughtered cows at follicular or luteal phases. Isolation of EV was performed using tangential flow filtration followed by size exclusion chromatography. EV were characterized by nanoparticle tracking analysis (NTA), fluorescence NTA, zeta potential, and transmission electron microscopy. Mass-spectrometry was used to evaluate EV protein profile from live cows. Particle concentrations (mean ± SD) were higher (P < 0.05) at follicular than at luteal phase in both live (1.01 × 108 ± 1.66 × 107 vs 7.56 × 107 ± 1.80 × 107, respectively) and slaughtered cows (1.17 × 108 ± 2.34 × 107 vs 9.12 × 107 ± 9.77 × 106, respectively). The proportion of fluorescently labelled EV varied significantly between follicular and luteal phases across live (28.9 ± 1.9% vs 19.3 ± 2.8%, respectively) and slaughtered cows (26.5 ± 6.3% vs 27.3 ± 2 .7%, respectively). In total, 41 EV proteins were differentially expressed between the phases. Some of the proteins were involved in reproductive processes, cell adhesion and proliferation, and cellular metabolic processes. The results indicated differences in bovine UF-EV concentration and protein profile at follicular and luteal phases, which would suggest that EV modulate uterine microenvironment across the oestrous cycle. Further research is needed to understand the effect of EV changes throughout the oestrous cycle.

show abstract

Insulin induces steroidogenesis in canine luteal cells via PI3K-MEK-MAPK

Silva

Cardoso

Giometti

et al. 2022

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Luteal expression of factors involved in the metabolism and sensitivity to oestrogens in the dog during pregnancy and in non‐pregnant cycle

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 8 publications

References 101 publications

An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum

An approach to uncover the relationship between 17b-estradiol and ESR1/ESR2 ratio in the regulation of canine corpus luteum

Characterization of bovine uterine fluid extracellular vesicles proteomic profiles at follicular and luteal phases of the oestrous cycle

Insulin induces steroidogenesis in canine luteal cells via PI3K-MEK-MAPK

Contact Info

Product

Resources

About