Lupinus angustifolius Protein Hydrolysates Reduce Abdominal Adiposity and Ameliorate Metabolic Associated Fatty Liver Disease (MAFLD) in Western Diet Fed-ApoE−/− Mice

Abstract

Metabolic-associated fatty liver disease (MAFLD) is the most important cause of liver disease worldwide. It is characterized by the accumulation of fat in the liver and is closely associated with abdominal obesity. In addition, oxidative stress and inflammation are significant features involved in MAFLD. Recently, our group demonstrated that lupin protein hydrolysates (LPHs) had lipid lowering, antioxidant, and anti-inflammatory effects. Sixty male mice fed with a Western diet were intragastrically treated with LPHs (or vehicle) for 12 weeks. Liver and adipose tissue lipid accumulation and hepatic inflammatory and oxidant status were evaluated. A significant decrease in steatosis was observed in LPHs-treated mice, which presented a decreased gene expression of CD36 and LDL-R, crucial markers in MAFLD. In addition, LPHs increased the hepatic total antioxidant capacity and reduced the hepatic inflammatory status. Moreover, LPHs-treated mice showed a significant reduction in abdominal adiposity. This is the first study to show that the supplementation with LPHs markedly ameliorates the generation of the steatotic liver caused by the intake of a Western diet and reduces abdominal obesity in ApoE−/− mice. Future clinical trials should shed light on the effects of LPHs on MAFLD.

Long non-coding RNA G23Rik attenuates fasting-induced lipid accumulation in the liver

“…Moreover, liver CHOL, NEFA, and TG levels were significantly elevated by fasting in G23Rik -/mouse livers when compared to fasted wildtype animals. Expression of Cd36, a PPARA-regulated long chain fatty acid transporter and an important marker for metabolic-associated fatty liver disease 29,30 , was significantly elevated in livers of fasting G23Rik -/mice compared to G23Rik +/+ mice, which may contribute to the observed hepatosteatosis in G23Rik -/livers. Thus, G23Rik has a negative regulatory effect on Cd36 expression under fasting conditions.…”

“…S3, Table S3). Furthermore, hepatic expression of Cd36, a long chain fatty acid transporter and PPARA target gene 29,30 , was significantly elevated in fasting G23Rik -/mice compared to fasting G23Rik +/+ mice (Fig. 5E).…”

Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice

“…The activity of these enzymes was elevated with MLPH treatment in a dose-dependent manner. Santos-Sanchez et al [ 57 ] also reported that treatment with Lupinus angustifolius protein hydrolysates boosted hepatic total antioxidant capacity, and then decreased hepatic inflammation and abdominal obesity in western diet-fed-apoE-/- mice. In the present study, the degradation of hepatic antioxidant enzyme activity in the CON group was effectively inhibited by MLPH treatment, suggesting that MLPH treatment is favorable to increase antioxidant effects in ob / ob mice.…”

Lupin-Derived Bioactive Peptides: Intestinal Transport, Bioavailability and Health Benefits

“…LDPs are also able to suppress lipid synthesis and accumulation and improve antioxidant and anti-inflammatory status after oral ingestion of LPHs by animal model of obesity [ 127 ]. Another useful observation has been provided by a recent clinical study that has shown that the consumption of 30 g/day lupin protein for three months reduced the plasma PCSK9 level by 12.7% [ 125 ].…”