Lupin γ-conglutin protects against cell death induced by oxidative stress and lipotoxicity, but transiently inhibits in vitro insulin secretion by increasing KATP channel currents

Guzmán, Tereso J.; Düfer, Martina; Wiedemann, Mats; Olguín‐Alor, Roxana; Soldevila, Gloria; Gurrola-Díaz, Carmen M.

doi:10.1016/j.ijbiomac.2021.07.088

Cited by 7 publications

(3 citation statements)

References 60 publications

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“…Such genes were related to different pathways: in the oxidative stress pathway, Ddit3 , Foxo1 , Foxo3 , Klf6 , Ppargc1a , and Serpine1 ; in the IL-6 signaling pathway, Map2k6 , Foxo1 , Foxo3 , and Jun ; in the Nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway, Map2k6 , Slc1a4 , Gstk1 , and Jun . These findings are in accordance with previous studies that analyzed the individual Cγ effect to reduce the proinflammatory response and insulin resistance in PANC-1 cells, through the modulation of INF-γ expression, cytokines (IL-6, IL-12P70, IL-17A, and IL-27), and ROS levels, as well as an increase in antioxidant molecules [ 20 , 58 ]. Furthermore, animal studies have reported that the intake of soybean proteins directly improves oxidative stress [ 59 , 60 , 61 ].…”

Section: Discussionsupporting

confidence: 93%

Combined Gamma Conglutin and Lupanine Treatment Exhibits In Vivo an Enhanced Antidiabetic Effect by Modulating the Liver Gene Expression Profile

et al. 2023

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Previous studies have individually shown the antidiabetic potential of gamma conglutin (Cγ) and lupanine from lupins. Until now, the influence of combining both compounds and the effective dose of the combination have not been assessed. Moreover, the resulting gene expression profile from this novel combination remains to be explored. Therefore, we aimed to evaluate different dose combinations of Cγ and lupanine by the oral glucose tolerance test (OGTT) to identify the higher antidiabetic effect on a T2D rat model. Later, we administered the selected dose combination during a week. Lastly, we evaluated biochemical parameters and liver gene expression profile using DNA microarrays and bioinformatic analysis. We found that the combination of 28 mg/kg BW Cγ + 20 mg/kg BW lupanine significantly reduced glycemia and lipid levels. Moreover, this treatment positively influenced the expression of Pdk4, G6pc, Foxo1, Foxo3, Ppargc1a, Serpine1, Myc, Slc37a4, Irs2, and Igfbp1 genes. The biological processes associated with these genes are oxidative stress, apoptosis regulation, and glucose and fatty-acid homeostasis. For the first time, we report the beneficial in vivo effect of the combination of two functional lupin compounds. Nevertheless, further studies are needed to investigate the pharmacokinetics and pharmacodynamics of the Cγ + lupanine combined treatment.

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Section: Discussionsupporting

confidence: 93%

Combined Gamma Conglutin and Lupanine Treatment Exhibits In Vivo an Enhanced Antidiabetic Effect by Modulating the Liver Gene Expression Profile

et al. 2023

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“…As shown in Figure 6A, 600 µM or higher H 2 O 2 concentrations decreased the percentage of living cells significantly compared with the control group. Considering that high H 2 O 2 concentrations may cause cell death by non-apoptotic mechanisms [59], 800 µM H 2 O 2 was selected to explore the protective effect of GSP-1a. According to Figure 6B, there was no significant difference in the viability of HepG2 cells when treated with a concentration range of 0 to 2 mg/mL of GSP-1a and GSP compared to the control group.…”

Section: Antioxidant Activities In Vitro Of Gsp-1amentioning

confidence: 99%

A Neutral Polysaccharide from Spores of Ophiocordyceps gracilis Regulates Oxidative Stress via NRF2/FNIP1 Pathway

Wang,

Wei,

Lian

et al. 2023

IJMS

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Ophiocordyceps gracilis (O. gracilis) is a parasitic fungus used in traditional Chinese medicine and functional foods. In this study, a neutral heteropolysaccharide (GSP-1a) was isolated from spores of O. gracilis, and its structure and antioxidant capacities were investigated. GSP-1a was found to have a molecular weight of 72.8 kDa and primarily consisted of mannose (42.28%), galactose (35.7%), and glucose (22.02%). The backbone of GSP-1a was composed of various sugar residues, including →6)-α-D-Manp-(1→, →2,6)-α-D-Manp-(1→, →2,4,6)-α-D-Manp-(1→, →6)-α-D-Glcp-(1→, and →3,6)-α-D-Glcp-(1→, with some branches consisting of →6)-α-D-Manp-(1→ and α-D-Gal-(1→. In vitro, antioxidant activity assays demonstrated that GSP-1a exhibited scavenging effects on hydroxyl radical (•OH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+), and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•). Moreover, GSP-1a was found to alleviate H2O2-induced oxidative stress in HepG2 cells by reducing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA), while enhancing the activities of superoxide dismutase (SOD). Furthermore, GSP-1a upregulated the mRNA expression of antioxidant enzymes such as Ho-1, Gclm, and Nqo1, and regulated the NRF2/KEAP1 and FNIP1/FEM1B pathways. The findings elucidated the structural types of GSP-1a and provided a reliable theoretical basis for its usage as a natural antioxidant in functional foods or medicine.

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“…In fact, several pieces of evidence highlight the biological activities of lupin protein hydrolysates and peptides, including hypocholesterolemic, hypoglycemic, antimicrobial, anti-inflammatory, and immunomodulatory effects. [2][3][4][5][6][7][8][9][10] The health-promoting effects of lupin peptides have been tested in different animal models and clinical trials. Briefly, diets containing protein from L. albus 11,12 or L. angustifolius [13][14][15] significantly reduced both total cholesterol and low-density lipoprotein (LDL) cholesterol (LDL-C) levels compared to control diets containing casein in a rat model of hypercholesterolemia.…”

Section: Introductionmentioning

confidence: 99%

A Lupinus angustifolius protein hydrolysate exerts hypocholesterolemic effects in Western diet-fed ApoE^−/− mice through the modulation of LDLR and PCSK9 pathways

et al. 2022

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Lupin γ-conglutin protects against cell death induced by oxidative stress and lipotoxicity, but transiently inhibits in vitro insulin secretion by increasing KATP channel currents

Cited by 7 publications

References 60 publications

Combined Gamma Conglutin and Lupanine Treatment Exhibits In Vivo an Enhanced Antidiabetic Effect by Modulating the Liver Gene Expression Profile

Combined Gamma Conglutin and Lupanine Treatment Exhibits In Vivo an Enhanced Antidiabetic Effect by Modulating the Liver Gene Expression Profile

A Neutral Polysaccharide from Spores of Ophiocordyceps gracilis Regulates Oxidative Stress via NRF2/FNIP1 Pathway

A Lupinus angustifolius protein hydrolysate exerts hypocholesterolemic effects in Western diet-fed ApoE^−/− mice through the modulation of LDLR and PCSK9 pathways

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Lupin γ-conglutin protects against cell death induced by oxidative stress and lipotoxicity, but transiently inhibits in vitro insulin secretion by increasing KATP channel currents

Cited by 7 publications

References 60 publications

Combined Gamma Conglutin and Lupanine Treatment Exhibits In Vivo an Enhanced Antidiabetic Effect by Modulating the Liver Gene Expression Profile

Combined Gamma Conglutin and Lupanine Treatment Exhibits In Vivo an Enhanced Antidiabetic Effect by Modulating the Liver Gene Expression Profile

A Neutral Polysaccharide from Spores of Ophiocordyceps gracilis Regulates Oxidative Stress via NRF2/FNIP1 Pathway

A Lupinus angustifolius protein hydrolysate exerts hypocholesterolemic effects in Western diet-fed ApoE−/− mice through the modulation of LDLR and PCSK9 pathways

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A Lupinus angustifolius protein hydrolysate exerts hypocholesterolemic effects in Western diet-fed ApoE^−/− mice through the modulation of LDLR and PCSK9 pathways