Lupin seeds lower plasma lipid concentrations and normalize antioxidant parameters in rats

Abstract

162in erythrocytes and plasma glutathione reductase (GR), glutathione-S-transferase (GST) and catalase (CAT) were examined. A hypercholesterolemia-induced diet manifested in the elevation of total lipids (TL), total cholesterol (TC), triglycerides (TG), LDL-C and MDA levels, ALT, AST, LDH activities and the depletion of GSH and enzymic antioxidants. The supplementation of a hypercholesterolemia-induced diet with bitter and sweet lupin seeds significantly lowered the plasma levels of TL, TC, TG and LDL-C. ALT, AST and LDH activities slightly decreased in treated groups compared with the hypercholesterolemic group (HC). Furthermore, the content of GSH significantly increased while MDA significantly decreased in treated groups compared with the HC group. In addition, the bitter lupin seed group improved enzymic antioxidants compared with the HC group. In general, the results indicated that the bitter lupin seed supplements are better than those containing sweet lupin seeds. These results suggested that the hypocholesterolemic effect of bitter and sweet lupin seed supplements might be due to their abilities to lower the plasma cholesterol level as well as to slow down the lipid peroxidation process and to enhance the antioxidant enzyme activity. KEY-WORDS: Bitter lupin -Hypercholesterolemia -Oxidative stress -Rats -Sweet lupin. INTRODUCTIONLegume seeds are an abundant source of proteins and, among them, lupin is one of the richest. Indeed, lupin seed deserves greater interest as a result of its chemical composition and increased availability in many countries in recent years. Lupin is a nonstarch leguminous seed with high protein content, almost as high as that of soybean (about 35% of the dry weight), relatively low oil content (Duranti et al., 2008) and a lack of antinutritional substances. The amount of antinutritional compounds found in other legumes, such as alkaloids, saponins, tannins and trypsin inhibitor, is minimal in lupin (Van Barneveld, 1999). Lupin cultivation is at least 2,000 years old and most likely began in Egypt or in the general Mediterranean region. The lupin plant, like other grain legumes (beans, peas, lentils, etc.) fixes atmospheric nitrogen, and produces seeds high in protein. There are over 300 species of the genus Lupinus (L.) (Putnam et al., 1989). Seeds of several species of lupins have been used as food for 3,000 years in the Mediterranean area (Gladstones, 1998). These bitter seeds had to be soaked in water RESUMEN Semillas de altramuces bajan la concentración de lípidos plasmáticos y normaliza los parámetros antioxidantes en ratas.Este estudio fue diseñado para evaluar semillas de altramuces dulces y amargas como agentes que bajan los lípidos y estudiar su efecto en la actividad antioxidante en ratas hipercolesterolémicas. El nivel de lípidos en plasma, malondialdehido (MDA) y glutatión reducido (GSH), así como la actividad transaminasa (ALT y AST), lactato deshidrogenasa (LHD) en plama, superóxido dismutasa (SOD) y glutatión peroxidasa (GPx) en eritrocitos, glutatión reductasa (GR)...

Effects of fermentation of narrow-leafed lupine (<i>L. angustifolius</i>) seeds on their chemical composition and physiological parameters in rats

“…In addition, in the group fed a diet involving raw lupine seeds, a significant decrease in total cholesterol and HDL cholesterol concentrations was found. Also other studies confirm hypocholesterolaemic advantages of lupine (Osman et al, 2011). The inhibition of cholesterol synthesis and cholesterol reduction in the blood could be affected by production of SCFA in digesta (Wong et al, 2006).…”

The Effect of Dehulling Lupine Seeds (Lupinus albus L.) from Low-Alkaloid Varieties on the Chemical Composition and Fatty Acids Content

“…The high value of the h/H index indicates the presence of valuable lipids, with a real potential to reduce the plasma cholesterol [36,55]. These hypocholesterolemic properties of lupine seeds have been demonstrated for rats [56]. https://doi.org /10.37358/Rev.…”

Influence of Functional Sweet White Lupin Biscuits on Lipid Profile and Food Efficiency of Induced Hyperlipidemia Rats

“…Moreover, El-Malky and Gouda (2007) indicated that germinated lupin seeds reduced the increase of serum glucose, TC, TG, LDL-c, VLDL-c and enhanced the increase of HDL-c. As well, Osman, Mahmoud, Romeilah and Fayed (2011) observed the supplementation of a hypercholesterolemia-induced diet with sweet lupin seeds significantly lowered the plasma levels of TC, TG and LDL-C. ALT, AST and LDH activities slightly decreased in treated groups compared with the hypercholesterolemic group (positive control).…”