Lung Uptake of Tc-99m HMPAO in Cigarette Smokers Expressed by Lung/Liver Activity Ratio

Wj, Shih; Rehm,; Günther, Frank; Jj, Coupal; Hj, Biersack; Berger, Rudolf; Yl, Lai; Uy, Ryo; Ml, Dillon

doi:10.1097/00003072-199303000-00010

Cited by 10 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hyperoxia-induced increase in 99m Tc-HMPAO lung uptake measured here is consistent with previous results which also demonstrate an increase in 99m Tc-HMPAO uptake in response to pulmonary oxidative stress (oxidant injury), including irradiation lung injury (22,25), inhalation injury (26,27) and cigarette smoking (28). Moreover, this is the first study to report on the pulmonary uptake of 99m Tc-duramycin in an animal model of disease or injury.…”

Section: Discussionsupporting

confidence: 92%

“…Clinical reports in patients with brain disorders, including psychiatric and movement disorders (13 -17) and stroke (18,19), have shown that HMPAO uptake is highly influenced by not only perfusion, but also by the nature of the lesion (20,21), suggesting its utility as a biomarker of cell-type specific metabolism. In addition, increases in 99m Tc-HMPAO uptake in the lung have been reported in subclinical lung injury due to chemotherapy (22), diffuse infiltrative lung disease (23), irradiation lung injury (24,25), and inhalation and smoking injuries (26-28) in humans. Of particular note is the observation that increases were observed in patients generally showing no abnormal opacity on chest radiographs or findings on pulmonary function tests (22,24,27).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential Lung Uptake of ^99mTc-Hexamethylpropyleneamine Oxime and ^99mTc-Duramycin in the Chronic Hyperoxia Rat Model

et al. 2012

View full text Add to dashboard Cite

Noninvasive radionuclide imaging has the potential to identify and assess mechanisms involved in particular stages of lung injury which occur with acute respiratory distress syndrome, for example. Lung uptake of 99mTc-hexamethylpropyleneamine oxime (HMPAO) is reported to be partially dependent on the redox status of the lung tissue while 99mTc-duramycin, a new marker of cell injury, senses cell death via apoptosis and/or necrosis. Thus, we investigated changes in lung uptake of these agents in rat exposed to hyperoxia for prolonged periods, a common model of acute lung injury. Methods Male Sprague-Dawley rats were pre-exposed to either normoxia (21% O2) or hyperoxia (85% O2) for up to 21 days. For imaging, the rats were anesthetized, injected i.v. with either 99mTc-HMPAO or 99mTc-duramycin (37-74 MBq) and planar images were acquired using a high sensitivity modular gamma camera. Subsequently, 99mTc-macroagreggated albumin (37 MBq, diam=10-40 μm) was injected i.v., imaged, and used to define a lung region-of-interest. The lung to background ratio was used as a measure of lung uptake. Results Hyperoxia exposure resulted in a 74% increase in 99mTc-HMPAO lung uptake, which peaked at 7 days and persisted for the 21 days of exposure. 99mTc-duramycin lung uptake was also maximal at 7 days of exposure but decreased to near control levels by 21 days. The sustained elevation of 99mTc-HMPAO uptake suggests ongoing changes in lung redox status whereas cell death appears to have subsided by 21 days. Conclusion These results suggest the potential use of 99mTc-HMPAO and 99mTc-duramycin as redox and cell-death imaging biomarkers, respectively, for in vivo identification and assessment of different stages of lung injury.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Differential Lung Uptake of ^99mTc-Hexamethylpropyleneamine Oxime and ^99mTc-Duramycin in the Chronic Hyperoxia Rat Model

et al. 2012

View full text Add to dashboard Cite

show abstract

“…For example, active smokers have increased uptake in lung that is proportional to the amount of cigarettes smoked. 163, 164 Furthermore, irradiation injury to the lung, which is directly related to ROS and RNS levels, is associated with markedly increased uptake of 99m Tc-HMPAO by the lung. 163, 164 Although the spatial resolution of clinical SPECT is relatively poor (~15 mm) in comparison to other modalities such as PET, it appears sufficient for quantitative assessment of regional airway redox and inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…163, 164 Furthermore, irradiation injury to the lung, which is directly related to ROS and RNS levels, is associated with markedly increased uptake of 99m Tc-HMPAO by the lung. 163, 164 Although the spatial resolution of clinical SPECT is relatively poor (~15 mm) in comparison to other modalities such as PET, it appears sufficient for quantitative assessment of regional airway redox and inflammation. Taken together, HMPAO-SPECT-CT may be an innovative imaging modality for the airway inflammation of asthma, as it is in cigarette smoke-related lung disease.…”

Section: Introductionmentioning

confidence: 99%

Lung imaging in asthmatic patients: The picture is clearer

Castro

Fain

Hoffman

et al. 2011

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

Imaging of the lungs in patients with asthma has evolved dramatically over the last decade with sophisticated techniques, such as computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET) and single photon emission computed tomography (SPECT). New insights into current and future modalities for imaging in asthma and their application are discussed to potentially shed a clearer picture of the underlying pathophysiology of asthma, especially severe asthma, and the proposed clinical utility of imaging in this common disease.

show abstract

Tc-99m-HMPAO uptake by bronchoalveolar cells

et al. 2003

View full text Add to dashboard Cite

Lung uptake of intravenously injected Tc-99m-HMPAO is observed in smokers and in lung toxicity due to various agents. We investigated the Tc-99m-HMPAO uptake of bronchoalveolar lavage (BAL) cells in the lungs after incubation in in vitro conditions (6 patients), intravenous injection (IV) (7 patients) and inhalation (INH) (6 patients) of Tc-99m-HMPAO in order to show whether BAL cells are also responsible for Tc-99m-HMPAO uptake in the lungs. Cell/supernatant (C/S) count ratio was 7.0 +/- 3.5, 29.3 +/- 40.8 and 8.4 +/- 4.5 for in vitro, IV and INH groups, respectively. C/Sin vitro showed a positive correlation with % alveolar macrophages (r = 0.943, p = 0.0048) and a negative correlation with % neutrophils (r = -0.945, p = 0.0045). Cells/whole BAL fluid ratio correlated with the amount of daily cigarette consumption in INH group (r = 0.95, p = 0.0037). Tc-99m-HMPAO showed adherence to mucus after inhalation. Tc-99m-HMPAO diffuses into alveolar spaces after injection and is present in BAL fluid and BAL cells both after injection and inhalation. Glutathione concentration and oxido-reductive state of the epithelial lining fluid and BAL cells may influence the lung uptake of Tc-99m-HMPAO.

show abstract

Lung Uptake of Tc-99m HMPAO in Cigarette Smokers Expressed by Lung/Liver Activity Ratio

Cited by 10 publications

References 0 publications

Differential Lung Uptake of ^99mTc-Hexamethylpropyleneamine Oxime and ^99mTc-Duramycin in the Chronic Hyperoxia Rat Model

Differential Lung Uptake of ^99mTc-Hexamethylpropyleneamine Oxime and ^99mTc-Duramycin in the Chronic Hyperoxia Rat Model

Lung imaging in asthmatic patients: The picture is clearer

Tc-99m-HMPAO uptake by bronchoalveolar cells

Contact Info

Product

Resources

About

Lung Uptake of Tc-99m HMPAO in Cigarette Smokers Expressed by Lung/Liver Activity Ratio

Cited by 10 publications

References 0 publications

Differential Lung Uptake of 99mTc-Hexamethylpropyleneamine Oxime and 99mTc-Duramycin in the Chronic Hyperoxia Rat Model

Differential Lung Uptake of 99mTc-Hexamethylpropyleneamine Oxime and 99mTc-Duramycin in the Chronic Hyperoxia Rat Model

Lung imaging in asthmatic patients: The picture is clearer

Tc-99m-HMPAO uptake by bronchoalveolar cells

Contact Info

Product

Resources

About

Differential Lung Uptake of ^99mTc-Hexamethylpropyleneamine Oxime and ^99mTc-Duramycin in the Chronic Hyperoxia Rat Model

Differential Lung Uptake of ^99mTc-Hexamethylpropyleneamine Oxime and ^99mTc-Duramycin in the Chronic Hyperoxia Rat Model