Lung tumors with mixed histologic pattern. Clinico-pathologic characteristics and prognostic significance

Ruffini, Enrico; Rena, Ottavio; Oliaro, A; Filosso, Pier Luigi; Bongiovanni, Massimo; Arslanian, Anna; Papalia, Esther; Maggi, G

doi:10.1016/s1010-7940(02)00481-5

Cited by 67 publications

(64 citation statements)

References 16 publications

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“…Previously, a previous study showed that mixed histological phenotypes were observed in 59 cases from 1,158 lung cancer patients (13). We also reported 21 cases with the mixed form of neuroendocrine tumors from 2,501 primary lung cancer cases (14).…”

Section: Discussionsupporting

confidence: 60%

Bone metastasis from lung cancer identified by genetic profiling

et al. 2016

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Abstract. Cancer metastasis remains responsible for the vast majority of cases of cancer-related morbidity and mortality. Metastasis, by its definition, is the spread of cancer from the primary site to the distant tissues. Advancing the scientific and clinical understanding of cancer metastasis is a high priority. The prerequisite requirement for pathological consistency may be compromised during metastasis. The present study reports the case of a cancer patient with different pathological types. The patient presented with pain in the neck and right hip, as well as weight loss. He underwent whole-body positron emission tomography-computed tomography, which identified a mass in the lung and abnormal metabolism of the bone. Biopsies of the ilium and lung were performed and he was shown to have lung adenocarcinoma and bone squamous carcinoma. The morphology and immunohistochemical patterns were completely different, while each lesion harbored an identical genetic profile. The bone lesion was identified to be a metastasis from the lung cancer. The patient was prescribed an epithelial growth factor receptor inhibitor, which resulted in a partial response in the lung mass and alleviation of the patient's bone pain. Through this case study, we advocate the importance of using genetic testing in addition to pathological assessment.

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Section: Discussionsupporting

confidence: 60%

Bone metastasis from lung cancer identified by genetic profiling

et al. 2016

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“…A lack of association in the immunohistochemical expression profile between lung biopsy specimens and the corresponding resected tumor specimens has been reported (27). Furthermore, intratumoral heterogeneity was shown not only in terms of microheterogeneity of the tumor cell phenotype (28), but in terms of genetic heterogeneity in cancer (29,30). In particular, the intratumoral genetic heterogeneity of EGFR mutations may explain the variable clinical response of NSCLC to gefitinib.…”

Section: Discussionmentioning

confidence: 99%

Prospective Study of the Accuracy of EGFR Mutational Analysis by High-Resolution Melting Analysis in Small Samples Obtained from Patients with Non–Small Cell Lung Cancer

Fukui

Ohe²,

Tsuta³

et al. 2008

Clinical Cancer Research

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Purpose: Epidermal growth factor receptor (EGFR) mutations, especially in-frame deletions in exon 19 (DEL) and a point mutation in exon 21 (L858R), predict gefitinib sensitivity in patients with non^small cell lung cancer (NSCLC). In this study, we verified the accuracy of EGFR mutation analysis in small samples by high-resolution melting analysis (HRMA), which is a rapid method using PCR amplification with a dye to analyze the melting curves in NSCLC. Experimental Design: We designed a prospective study to compare the sensitivity and specificity of HRMA and DNA sequencing with laser capture microdissection. Eligible patients with lung lesions were screened by bronchoscopy or percutaneous needle biopsy to histologically confirm the diagnosis, followed by surgical resection of the NSCLC. Small diagnostic specimens were analyzed for EGFR mutations by HRMA, and the surgically resected specimens were examined for mutations by HRMA and DNA sequencing. Results:The analyses for EGFR mutations were conducted in 52 eligible cases of the 92 enrolled patients. EGFR mutations were detected in 18 (34.6%) patients. The results of HRMA from surgically resected specimens as well as DNA sequencing revealed 100% sensitivity and specificity. On the other hand, the sensitivity and specificity of HRMA from the small diagnostic specimens were 83.3% and 100%, respectively. Conclusions: In this study, we showed that HRMA is a highly accurate method for detecting DEL and L858R mutations in patients with NSCLC, although it is necessary to consider the identification of patients with a false-negative result when the analysis is conducted using small samples.

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“…Combined small cell lung cancer with non-small cell component is a rarely occurring lung tumor which is thought to be characterized by more aggressive clinico-pathologic behaviour than histologically pure tumors [22]. Here, we present a cell line derived from a 54-year-old woman who was diagnosed with SCLC/NSCLC (stage IV, extensive disease).…”

Section: Discussionmentioning

confidence: 99%

“…Another receptor that was detected on a subpopulation of the STP54 cells was CD210, a receptor for interleukin 10. The role of this cytokine in SCLC has not been described, however there are some contradictory papers of its impact on NSCLC [22,23]. Latent Membrane Protein-1 (LMP-1), a molecule characteristic for Epstein-Barr Virus infection was found on the STP54 cells.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a new small cell lung cancer (SCLC) cell line STP54 derived from a metastatic bioptate of a combined type of SCLC with Non-SCLC component.

Skirecki

Hoser²,

Domagała‐Kulawik³

et al. 2009

Folia Histochem Cytobiol.

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Materials and methodsPatient history and biopsy specimen. A 54-year-old, smoking woman was admitted to the hospital due to enlarged opacity in the X-ray film of the upper mediastinum, reccuring fevers, cough and exertional dyspnoea. The patient gave a several months history of cough and pyrexia prior to hospitalization. Physical examination revealed palpable 3 cm supraclavicular tumor. CT scan revealed 10cm mass in the right mediastinum, fusing with two others. On bronchoscopy micronodular lesions of the right brochi were present. Serum LDH concentration was markedly increased. In order to establish a diagnosis a fine-needle aspiration biopsy (FNAB) of supraclavicular lymph node was taken. The aspirate was split. One part was fixed in ethanol for cytological examintation and the other was placed in sterile physiological saline for culturing. The examination of FNAB smear in the light microscopy revealed cancer cells with the features of SCLC and NSCLC and finally combined type of SCLC was diagnosed. After completion of diagnosis the patient was discharged from the hospital in a good general condition and recommended for treatment in oncology center. Abstract: Small cell lung cancer constitutes 15-20% cases of lung cancers, currently the leading cause of death from malignant diseases. It also causes the demise of >90% of affected individuals in 5 years. We have established a new SCLC cell line STP54 derived from fine needle aspirate of metastatic supraclavicular lymph node of 54 -year-old women for model experiments. The primary tumor was diagnosed by histopathological examination as combined type of small cell lung cancer with a non-small cell component. We cultured the cancer cells in the RPMI 1640 medium. In the long-term culture only the small cell component survived. The cell line was established after 30 passages and then characterized by performing cell morphology, cell growth analysis, tumorigenicity in vitro and flow cytometry analysis of selected markers (like NCAM, cytokeratines, HLA-ABC, Fas, Bcl-2, p53, CXCR4, CD210). The cells were growing in floating aggregates and show features suggesting its invasiveness. We suggest that this new cell line may serve as a valuable tool for further studies on lung tumor biology, molecular pathogenesis and metastatic mechanism. FOLIA HISTOCHEMICAET CYTOBIOLOGICA Vol. 47, No. 1,

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Lung tumors with mixed histologic pattern. Clinico-pathologic characteristics and prognostic significance

Abstract: Lung tumors with mixed histologic pattern are rare tumors. Adenosquamous carcinoma and combined SCLC + LCNEC/NNEC present a more aggressive clinico-pathologic behaviour and reduced survival as compared to the single histology population of resected lung tumors.

Cited by 67 publications

References 16 publications

Bone metastasis from lung cancer identified by genetic profiling

Bone metastasis from lung cancer identified by genetic profiling

Prospective Study of the Accuracy of EGFR Mutational Analysis by High-Resolution Melting Analysis in Small Samples Obtained from Patients with Non–Small Cell Lung Cancer

Characterization of a new small cell lung cancer (SCLC) cell line STP54 derived from a metastatic bioptate of a combined type of SCLC with Non-SCLC component.

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