Lung colony formation: A selective cloning process for lung-colony-forming ability

Suzuki, Norio

doi:10.1038/bjc.1979.31

Cited by 8 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the affinity of tumour cells for the lung is the prime determinant of these differences, the clone FSA1233, which had a higher LCFE than FSA1231, would have a higher frequency of spontaneous metastasis to the lung. The important factor(s) in this system remain to be clarified, although the metastatic process requires other factors than those involved in local growth (Suzuki & Withers, 1979; present results).…”

Section: Methodsmentioning

confidence: 70%

Malignant properties and DNA content of daughter clones from a mouse fibrosarcoma: differentiation between malignant properties

Suzuki¹,

Williams²,

Hunter³

1980

Br J Cancer

Self Cite

View full text Add to dashboard Cite

Summary.-Freshly isolated clones of high cloning efficiency from a mouse fibrosarcoma were examined for DNA content, cell size, protein content, and malignant characteristics such as artificial lung-colony-forming ability, s.c. tumour take, host survival, and spontaneous metastatic ability.These malignant characteristics and other cell properties were heterogeneous among these clones; the malignant characteristics could vary and were not "all or none" in their nature. The higher the DNA content or the larger the cell volume, the higher the malignancy in terms of artificial lung-colony-forming efficiency, s.c. tumour take, and host survival.Despite variability of each parameter, the ratio of DNA content to cell size or protein content remained constant through these variations: the increased DNA paralleled increased protein and increased cell volume. The increased DNA was correlated with the more malignant characteristics of local growth and lung-colonyforming efficiency.Spontaneous metastasis to the lung was totally different from the local growth abilities; the small-cell clone produced more metastases.The graded nature of malignant properties and the differentiation between local growth and metastatic potential among the daughter clones indicate that malignancy reflects a complex moiety of cell properties.

show abstract

Section: Methodsmentioning

confidence: 70%

Malignant properties and DNA content of daughter clones from a mouse fibrosarcoma: differentiation between malignant properties

Suzuki¹,

Williams²,

Hunter³

1980

Br J Cancer

Self Cite

View full text Add to dashboard Cite

show abstract

“…1A-C) in mouse lungs, suggesting that apoptosis is at least one of the mechanisms accounting for the cell death of KHT-C cells in lungs. We could not measure the extent of apoptosis of KHT cells in mouse lungs or how much this apoptosis is affected by hypoxia due to the technical difficulties associated with the noisy background of lung section fluorescent microscopy and the limited KHT-C cell number (maximum 10 5 cells/mouse) to be injected without changing the dynamics of the tumor cell metastatic potential (53). However, studies on the expression of p53-regulated apoptotic genes have shown an attenuated response of hypoxia-pretreated KHT cells to ionizing radiation (Fig.…”

Section: Discussionmentioning

confidence: 99%

Hypoxia Enhances Metastatic Efficiency by Up-Regulating Mdm2 in KHT Cells and Increasing Resistance to Apoptosis

Zhang

Hill²

2004

Cancer Research

View full text Add to dashboard Cite

Tumor hypoxia has been reported to be a negative prognostic factor in a number of tumor sites. Both clinical and experimental studies have suggested a positive correlation between tumor hypoxia and increased metastatic efficiency; however, the mechanisms are not understood. In this study, the mechanisms of hypoxia-enhanced metastasis have been investigated in murine KHT fibrosarcoma and SCC VII cells. We have observed that hypoxia-pretreated KHT-C cells have a higher survival rate than control KHT-C cells after being arrested in mouse lungs. cDNA microarray analysis revealed many hypoxia-regulated genes, most of which have been reported to be involved in cell survival and growth. Among these genes, we have confirmed the up-regulation of Mdm2 by hypoxia and have demonstrated that this up-regulation is p53 independent. The up-regulation of Mdm2 by hypoxia is associated with decreased p53 protein and inhibition of the transactivation of p53 downstream proapoptotic genes. Overexpression of Mdm2 or suppression of p53 by transient transfection increased metastatic efficiency in KHT-C cells. These data suggest that hypoxia can increase tumor cell metastatic efficiency by rendering the tumor cells less sensitive to stress-induced cell death, e.g., through modifying the levels of Mdm2 and p53.

show abstract

“…Animal model research into bone metastasis is starting to play an important role in dealing with this challenge. Several in vivo and in vitro experimental methods have been developed that allow for the isolation of subpopulations of cancer cells that have a high metastatic potential to specific organs (6)(7)(8)(9)(10)(11). However, in vivo selection systems are timeconsuming and expensive.…”

Section: Discussionmentioning

confidence: 99%

Matrix metalloproteinase-1 is a crucial bone metastasis factor in a human breast cancer-derived highly invasive cell line

Okuyama

Matsumine²,

Kosugi³

et al. 1994

Oncol Rep

View full text Add to dashboard Cite

Abstract. Bone metastasis is one of the most severe cancer complications. To analyze the mechanism of bone metastasis, we established highly invasive cell lines from the human breast cancer cell line MDA-MB-231 using an in vitro sequential selection system. The cell lines, MDA-231-S10 and MDA-231-S5, were more invasive and more motile than the parental cell line. Moreover, MDA-231-S10 metastasized to bone more often when inoculated into the arterial circulation of nude mice. MDA-231-S10-bearing nude mice had a significantly poorer prognosis, and their bony metastatic tumors grew more rapidly than those of the mice bearing the parental cell line (MDA-231-P). Given that a high expression of matrix metalloproteinase (MMP) is reported to be associated with cancer invasiveness, we examined MMP expression. Our results showed that the expression of MMP-3, -5, -7, -9, -13 and -14 was decreased on Multiplex real-time quantitative RT-PCR analysis in the two new cell lines. The zymographic analysis showed no MMP-2 activity and a decreased MMP-9 activity in MDA-231-S10. However, the expression of MMP-1 in MDA-231-S10 was increased. We therefore concluded that MMP-1 plays a crucial role in breast cancer bone metastasis. Furthermore, our MDA-231-derived cell lines are useful analytical models of MMP-1-associated breast cancer bone metastasis. IntroductionBone metastasis frequently occurs in advanced breast cancer patients. Among patients with metastatic breast cancers, 70% have skeletal involvement (1). It has also been observed that patients with predominantly bone metastases have a worse prognosis than those with soft tissue metastases, because the bone metastases are more resistant to endocrine therapy and chemotherapy than metastases to non-bone organs (2,3). Bone metastases cause severe complications, such as intractable bone pain, pathological fractures and hypercalcemia, which can have a largely negative impact on the patient quality of life. Therefore, controlling bone metastases is of major clinical importance.The mechanisms of breast cancer bone metastasis remain poorly understood. In part, this is due to the lack of a suitable animal bone metastasis experimental model. To be able to analyze the mechanism of bone metastasis, a human cancer cell line that frequently metastasizes to bone is needed. In 1973, MDA-MB-231, a human breast cancer cell line, was established by Cailleau et al (4). Since it metastasizes to bone when injected into the arterial circulation of nude mice (5), we postulated that this cell line may contain subpopulations of cells that have differing potentials of metastasizing to bone. Therefore, we attempted to isolate an MDA-MB-231-derived highly invasive breast cancer cell line using a matrigel invasion chamber.Several in vivo and in vitro experimental methods have been developed that enable one to isolate subpopulations of cancer cells with a high metastatic potential to a specific organ (6-11). However, these in vivo selection systems are timeconsuming and expensive. In contrast, our method whi...

show abstract

Lung colony formation: A selective cloning process for lung-colony-forming ability

Cited by 8 publications

References 13 publications

Malignant properties and DNA content of daughter clones from a mouse fibrosarcoma: differentiation between malignant properties

Malignant properties and DNA content of daughter clones from a mouse fibrosarcoma: differentiation between malignant properties

Hypoxia Enhances Metastatic Efficiency by Up-Regulating Mdm2 in KHT Cells and Increasing Resistance to Apoptosis

Matrix metalloproteinase-1 is a crucial bone metastasis factor in a human breast cancer-derived highly invasive cell line

Contact Info

Product

Resources

About