Lumpy skin disease outbreaks in Greece during 2015–16, implementation of emergency immunization and genetic differentiation between field isolates and vaccine virus strains

Agianniotaki, Eirini I.; Tasioudi, Konstantia E.; Chaintoutis, Serafeim C.; Iliadou, Peristera; Mangana-Vougiouka, O; Kirtzalidou, Aikaterini; Alexandropoulos, Thomas; Sachpatzidis, Achilleas; Plevraki, E.; Dovas, Chrysostomos Ι.; Chondrokouki, Eleni

doi:10.1016/j.vetmic.2016.12.037

Cited by 64 publications

(62 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the effectiveness of the real‐time PCR assay to differentiate the vaccine virus from the field virus, 13 amplicons obtained from 10 blood samples and three aspirates belonging to 10 vaccinated animals from the study herd were sequenced for verification purposes. The Evros/GR/15 LSDV isolate was used as WT control (Agianniotaki et al., ), and DNA extracts from both LSD vaccines (OBP and MSD) were used as vaccine controls. Additional DNA blood extracts from 39 characterized WT LSDV‐positive cattle, originating from the 2015–2016 Greek epizootics, were also used for further evaluation of the real‐time PCR method.…”

Section: Methodsmentioning

confidence: 99%

“…The Evros/GR/15 LSDV isolate was used as WT control (Agianniotaki et al, 2017), and DNA extracts from both LSD vaccines (OBP and MSD) were used as vaccine controls. Additional DNA blood extracts from 39 characterized WT LSDV-positive cattle, originating from the 2015-2016 Greek epizootics, were also used for further evaluation of the real-time PCR method.…”

Section: Virological Testingmentioning

confidence: 99%

See 1 more Smart Citation

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Katsoulos

Chaintoutis

Dovas

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

SummaryThe present study was performed to investigate the clinical impact and certain virological and haematological parameters following immunization of cattle against lumpy skin disease (LSD). The study was conducted in a dairy cattle farm (215 animals), immunized with a Neethling strain-based live vaccine. Twenty-seven animals (14 lactating cows, four dry cows and nine calves) were randomly selected for repetitive blood and saliva samplings. An EvaGreen-based real-time PCR was designed to differentiate vaccine from field LSDVs. Vaccinated animals underwent examination for adverse reactions. Nodule samples were collected from two representative cases for histopathological testing and virus identification. Milk yield was calculated based on bulk-tank measurements of all lactating cows (79). Viral DNA was detected between days 6-15 post-vaccination (p.v.) at 63% of the sampled animals (17/27). Saliva and bulk-tank milk samples were LSDV-negative. Pronounced swelling was observed at injection sites of 12% of the immunized animals (26/215), starting at day 6 p.v., and was resolved after 2-4 days. Small-sized (<0.5 cm) cutaneous lumps were developed between days 8-18 p.v. at 9% of the vaccinated animals (19/215). These were observed in adult cows and not in calves/heifers.Resolution was observable 10 days post-development. The vaccine virus was also identified in nodules and injection-site aspirates. Haematological changes (e.g., lower leucocyte counts) were observed in cows and not in calves. Daily milk production was being reduced during the first 12 days p.v. LSD immunization of cows resulted in nodules and low viraemia levels. The fact that nodules and haematological changes were not observed in calves, along with the low viraemia, supports the reduced virulence of the Neethling vaccine strain. The characteristic nodules in vaccinated animals could allow clinical differentiation from those observed in LSD. The developed real-time PCR efficiently differentiates infected from vaccinated cattle, and should be further validated as a tool in LSD surveillance. † The first two authors contributed equally to this work.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Virological Testingmentioning

confidence: 99%

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Katsoulos

Chaintoutis

Dovas

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Capripoxviruses are important causative agents for many diseases of domestic and wild ruminants, including sheep pox, goat pox, and cattle lumpy skin disease (LSD). Goat pox and sheep pox have been endemic in many countries of Africa and Eurasia for a long time, while LSD has spread from Africa to southern and eastern Europe, the Middle East, and Central Asia in recent years; furthermore, Southeast and East Asia currently face an imminent threat (20,21). Although capripoxviruses have a huge impact on domestic animal husbandry worldwide, there are few detailed studies of the molecular basis of their virulence and pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

The 135 Gene of Goatpox Virus Encodes an Inhibitor of NF-κB and Apoptosis and May Serve as an Improved Insertion Site To Generate Vectored Live Vaccine

Sun

Chen

2018

J Virol

View full text Add to dashboard Cite

Goatpox virus (GTPV) is an important member of the genus of the Capripoxviruses have large and complex DNA genomes encoding many unknown proteins that may contribute to virulence. We identified that the open reading frame of GTPV is an early gene that encodes an ∼18-kDa protein that is nonessential for viral replication in cells. This protein functioned as an inhibitor of NF-κB activation and apoptosis and is similar to the N1L protein of vaccinia virus. In the natural host, sheep, deletion of the gene from the GTPV live vaccine strain AV41 resulted in less attenuation than that induced by deletion of the gene, a well-defined nonessential gene in the poxvirus genome. Using the gene as the insertion site, a recombinant AV41 strain expressing hemagglutinin of peste des petits ruminants virus (PPRV) was generated and elicited stronger neutralization antibody responses than those obtained using the traditional gene as the insertion site. These results suggest that the gene of GTPV encodes an immunomodulatory protein to suppress host innate immunity and may serve as an optimized insertion site to generate capripoxvirus-vectored live dual vaccines. Capripoxviruses are etiological agents of important diseases in sheep, goats, and cattle. There are rare reports about viral protein function related to capripoxviruses. In the present study, we found that the 135 protein of GTPV plays an important role in inhibition of innate immunity and apoptosis in host cells. Use of the gene as the insertion site to generate a vectored vaccine resulted in stronger adaptive immune responses than those obtained using the locus as the insertion site. As capripoxviruses are promising virus-vectored vaccines against many important diseases in small ruminants and cattle, the gene may serve as an improved insertion site togenerate recombinant capripoxvirus-vectored live dual vaccines.

show abstract

“…The LSDV isolate Evros/GR/15 was isolated from a biopsy tissue specimen as previously described (5). DNA was purified from cell culture supernatant using a Puregene Core Kit A (Qiagen) according to the manufacturer’s instructions.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Lumpy skin disease outbreaks in Greece during 2015–16, implementation of emergency immunization and genetic differentiation between field isolates and vaccine virus strains

Cited by 64 publications

References 17 publications

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

Investigation on the incidence of adverse reactions, viraemia and haematological changes following field immunization of cattle using a live attenuated vaccine against lumpy skin disease

The 135 Gene of Goatpox Virus Encodes an Inhibitor of NF-κB and Apoptosis and May Serve as an Improved Insertion Site To Generate Vectored Live Vaccine

Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

Contact Info

Product

Resources

About