Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

Arrabito, Giuseppe; Galati, C.; Castellano, Sabrina; Pignataro, Bruno

doi:10.1039/c2lc40948h

Cited by 33 publications

(40 citation statements)

References 28 publications

(37 reference statements)

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“…A challenge in this field is that the droplets move and mix in solution, and a chemical tracker is therefore typically included in the drop for identification. Droplet microarrays provide a different solution to this technical challenge by attaching the droplet to a surface, so that its composition is known by its position in the array, at the cost of limiting the array to two dimensions (Gosalia and Diamond, 2003; Mugherli et al, 2009; Arrabito et al, 2013; Sun et al, 2015; Popova et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Screening of Lipid Composition for Scalable Fabrication of Solvent-Free Lipid Microarrays

Ghazanfari

Lenhert

2016

Front. Mater.

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Liquid microdroplet arrays on surfaces are a promising approach to the miniaturization of laboratory processes such as high-throughput screening. The fluid nature of these droplets poses unique challenges and opportunities in their fabrication and application, particularly for the scalable integration of multiple materials over large areas and immersion into cell culture solution. Here, we use pin spotting and nanointaglio printing to screen a library of lipids and their mixtures for their compatibility with these fabrication processes, as well as stability upon immersion into aqueous solution. More than 200 combinations of natural and synthetic oils composed of fatty acids, triglycerides, and hydrocarbons were tested for their pin-spotting and nanointaglio print quality and their ability to contain the fluorescent compound tetramethylrhodamine B isothiocyanate (TRITC) upon immersion in water. A combination of castor oil and hexanoic acid at the ratio of 1:1 (w/w) was found optimal for producing reproducible patterns that are stable upon immersion into water. This method is capable of large-scale nanomaterials integration.

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Section: Introductionmentioning

confidence: 99%

Screening of Lipid Composition for Scalable Fabrication of Solvent-Free Lipid Microarrays

Ghazanfari

Lenhert

2016

Front. Mater.

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“…Pignataro et al employed inkjet printing for the fabrication of sub-nanoliter droplet-to-droplet arrays. 2,3 In this method, due to the high hygroscopicity of glycerol, liquid spots are stable during both the multilayer assembly and the execution of the assay. However, none of these printing methods can provide precise and reliable multistep droplet dispensing while exhibiting all of the following features: non-contact liquid dispensing capability, absence of evaporation issues without the assistance of additives, and capability of multistep/ sequential droplet assembly.…”

Section: Introductionmentioning

confidence: 99%

“…2,3 Uchiyama et al combined the inkjet technology with a multicapillary plate to develop a chemiluminescence immunoassay. 4 Despite these achievements, however, inkjet-based array formation is still not being widely applied in chemical and biological research.…”

Section: Introductionmentioning

confidence: 99%

“…Evaporation effects typically prevent the extreme scale reduction of droplet reactions to picoliter volumes. Specific strategies have been devised to address this problem, including the introduction of an ultrasonic humidifier 5,6 and the use of additive chemicals such as glycerol 3,7 and dimethyl sulfoxide (DMSO) 8 to maintain constant water content. However, these approaches run the risk of cross-contamination or suffer from limitations due to incompatibility with enzyme species, which limits the applicability of inkjet printing for most screening assays.…”

Section: Introductionmentioning

confidence: 99%

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Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing

et al. 2015

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We introduce a new model to describe the multiple printing procedure implemented by the inkjet printing approach. This non-contact and sequential picoliter droplet printing technology is named as sequential inkjet printing.Please check this proof carefully. Our staff will not read it in detail after you have returned it.Translation errors between word-processor files and typesetting systems can occur so the whole proof needs to be read. Please pay particular attention to: tabulated material; equations; numerical data; figures and graphics; and references. If you have not already indicated the corresponding author(s) please mark their name(s) with an asterisk. Please e-mail a list of corrections or the PDF with electronic notes attached -do not change the text within the PDF file or send a revised manuscript. Corrections at this stage should be minor and not involve extensive changes. All corrections must be sent at the same time.Please bear in mind that minor layout improvements, e.g. in line breaking, table widths and graphic placement, are routinely applied to the final version.Please note that, in the typefaces we use, an italic vee looks like this: n, and a Greek nu looks like this: ν.We will publish articles on the web as soon as possible after receiving your corrections; no late corrections will be made.Please return your final corrections, where possible within 48 hours of receipt, by e-mail to: loc@rsc.org Queries for the attention of the authors Journal: Lab on a Chip Paper: c5lc00356cTitle: Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing Editor's queries are marked on your proof like this Q1 , Q2 , etc. and for your convenience line numbers are indicated like this 5, 10, 15, ... Please ensure that all queries are answered when returning your proof corrections so that publication of your article is not delayed. In recent years, inkjet printing, as a new method to fabricate microdroplet microarrays, has been increasingly applied in the field of biochemical diagnostics. To further improve the general applicability of the inkjet printing technology in fabricating biochemical chips, in this work, we introduce a model to describe the multiple injection procedure implemented by the inkjet printing approach, with experimental verification. The multiple injection model demonstrates a new sequential inkjet printing method that generates picoliter-scale multicomponent droplet-in-oil arrays via multistep printing on uniform planarsubstrates. Based on our previous work on double-inkjet printing, this technique adapts the piezoelectric inkjet printing technology to fabricate an oil droplet array, into which multiple precise injections of secondary droplets with different compositions and volumes can be automatically printed in the required sequence, simultaneously addressing the evaporation issues associated with printing picoliter droplets without external assistance. In this paper, we first describe the theory and characterize the model, which account for the basic principles ...

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“…Nowadays, the immunoaffinitiy-based assays are the methods of choice owing to the high sensitivity and specificity of the antibody-antigen interaction [2][3][4] . These include wellestablished techniques such as lateral flow assays (routinely used for urine analysis), and enzyme-linked immunosorbent assays (ELISAs) 5 .…”

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confidence: 99%

Active accumulation of very diluted biomolecules by nano-dispensing for easy detection below the femtomolar range

et al. 2014

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Highly sensitive detection of biomolecules is of paramount interest in many fields including biomedicine, safety and eco-pollution. Conventional analyses use well-established techniques with detection limits B1 pM. Here we propose a pyro-concentrator able to accumulate biomolecules directly onto a conventional binding surface. The operation principle is relatively simple but very effective. Tiny droplets are drawn pyro-electro-dynamically and released onto a specific site, thus increasing the sensitivity. The reliability of the technique is demonstrated in case of labelled oligonucleotides diluted serially. The results show the possibility to detect very diluted oligonucleotides, down to a few hundreds of attomoles. Excellent results are shown also in case of a sample of clinical interest, the gliadin, where a 60-fold improved detection limit is reached, compared with standard ELISA. This method could open the way to a mass-based technology for sensing molecules at very low concentrations, in environmental as well as in diagnostics applications.

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Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

Cited by 33 publications

References 28 publications

Screening of Lipid Composition for Scalable Fabrication of Solvent-Free Lipid Microarrays

Screening of Lipid Composition for Scalable Fabrication of Solvent-Free Lipid Microarrays

Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing

Active accumulation of very diluted biomolecules by nano-dispensing for easy detection below the femtomolar range

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