Luminometer development in the last four decades: recollections of two entrepreneurs

Berthold, Fritz; Tarkkanen, Veikko

doi:10.1002/bio.2405

Cited by 8 publications

(8 citation statements)

References 24 publications

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“…Equation (1) shows that the signal is approximately linear with the rate of signal photons received, while it is inversely proportional to the square root of the dark current. With dark current proportional to the sensor area, the ideal sensor size is achieved when further size increases result in only a square root increase in photocurrent.…”

Section: Choice Of Sensor Sizementioning

confidence: 99%

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel

Elledge²,

Wiener

et al. 2023

Preprint

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Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although low light detection is well-established in centralized laboratory settings, the cost, size, and environmental requirements of high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present the design and application of a ~$700 US handheld, battery-powered luminometer with performance on par with high-end benchtop instruments. By pairing robust and inexpensive Silicon Photomultiplier (SiPM) sensors with a low-profile shutter system, our design compensates for sensor non-idealities and thermal drift, achieving a limit of detection of 1.6E-19 moles of firefly luciferase, or approximately 1fW of radiant optical power. Using these devices, we performed split luciferase serology studies to monitor sars-cov-2 antibodies in a cohort in the United States, as well as a field study in Bangladesh.

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Section: Choice Of Sensor Sizementioning

confidence: 99%

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel

Elledge²,

Wiener

et al. 2023

Preprint

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“…Considering these premises, the definition of the impact of NP physicochemical properties on cell internalization mechanisms, together with the characterization of biological cell response might pave the way for effective NP-delivery solutions for cell tracking, finally providing future perspectives for a bench to bedside translation (Figure 8). Supplementary Materials: References [244][245][246][247] are cited in the supplementary materials. The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Table S1: Clinical trial studies investigating the use of magnetic NPs for cell tracking; Table S2: Database of citations exploring direct cell tracking approaches through imaging; Table S3: General operational principles of the technologies used for in vitro cell tracking; Table S4: General operational principles of the technologies used for in vivo cell tracking.…”

Section: Conclusion and Outlook For Effective Np-based Cell Trackingmentioning

confidence: 99%

“…The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/nano12091414/s1, Table S1: Clinical trial studies investigating the use of magnetic NPs for cell tracking; Table S2: Database of citations exploring direct cell tracking approaches through imaging; Table S3: General operational principles of the technologies used for in vitro cell tracking; Table S4: General operational principles of the technologies used for in vivo cell tracking. References [244][245][246][247] are cited in the supplementary materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Nanotechnology-Assisted Cell Tracking

et al. 2022

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The usefulness of nanoparticles (NPs) in the diagnostic and/or therapeutic sector is derived from their aptitude for navigating intra- and extracellular barriers successfully and to be spatiotemporally targeted. In this context, the optimization of NP delivery platforms is technologically related to the exploitation of the mechanisms involved in the NP–cell interaction. This review provides a detailed overview of the available technologies focusing on cell–NP interaction/detection by describing their applications in the fields of cancer and regenerative medicine. Specifically, a literature survey has been performed to analyze the key nanocarrier-impacting elements, such as NP typology and functionalization, the ability to tune cell interaction mechanisms under in vitro and in vivo conditions by framing, and at the same time, the imaging devices supporting NP delivery assessment, and consideration of their specificity and sensitivity. Although the large amount of literature information on the designs and applications of cell membrane-coated NPs has reached the extent at which it could be considered a mature branch of nanomedicine ready to be translated to the clinic, the technology applied to the biomimetic functionalization strategy of the design of NPs for directing cell labelling and intracellular retention appears less advanced. These approaches, if properly scaled up, will present diverse biomedical applications and make a positive impact on human health.

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“…There were several early attempts to quantify the activity of the microbiome based on chemical reactions [30,88]. Detection and quantifying ATP can be used as an estimation of biological activity on surfaces like paper, paintings, or other materials in swab samples using the commercially-available products of luminometers [89,90]. However, the active microbial groups and members on cultural heritage may not be the directly destructive ones responsible for the damage caused to the cultural heritage and the general purpose-driven biochemical analysis of the samples from cultural heritage only provides limited insights because a close relationship between the destruction and specific microbial group cannot be established.…”

Section: Biochemical Functional Approachesmentioning

confidence: 99%

A Review on Sampling Techniques and Analytical Methods for Microbiota of Cultural Properties and Historical Architecture

Ding

Lan

2020

Applied Sciences

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World cultural heritage suffers from deterioration caused by both natural and anthropogenic processes, among which microorganisms are significantly involved. Among the key issues of this topic, sampling techniques and analytical methods for revealing the microbiome are fundamental to obtaining useful results for understanding the key players and processes involved, and also for effective protection and management of the cultural heritage for humanity. A non-invasive and non-destructive sampling method is required for sampling of cultural properties prior to further analysis of the microbiome. One example is illustrated in this article. For many years, culture-dependent methods had been used before the invention of polymerase-chain reaction (PCR) methods and, more recently, specifically high-throughput next generation sequencing (NGS). NGS reveals the whole microbial community composition and the active microorganisms from genomic DNA and RNA, respectively. The recovered environmental DNA and RNA from samples provide the information on microbial community and composition, and the active members and biochemical processes of the microbial attributes. It should be emphasized that the metabolically-active members of functional microflora in the biofilm or microbiome on cultural heritage must be determined and identified from the RNA-based analysis to gain a substantially important insight of the active biodeterioration processes and also the effectiveness of the conservation strategies. The importance of the culture-independent technique, based on NGS, is that it can be used in combination with the conventional culturing methods to guide the isolation and enrichment of new microorganisms to gain further biochemical insights to advance the role of the specific microbial groups for biodeterioration of cultural heritage. At the same time, effective restoration and maintenance strategies can be formulated for the protection of world cultural heritage.

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Luminometer development in the last four decades: recollections of two entrepreneurs

Cited by 8 publications

References 24 publications

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Nanotechnology-Assisted Cell Tracking

A Review on Sampling Techniques and Analytical Methods for Microbiota of Cultural Properties and Historical Architecture

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