Luminescent Transition Metal Complexes as Biological Labels and Probes

Lo, Kenneth Kam‐Wing

doi:10.1007/430_2006_040

Cited by 41 publications

(14 citation statements)

References 73 publications

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“…Luminescent transition metal complexes have received increasing attention in photochemistry, organic optoelectronics and luminescent sensors ( 26–33 ). We previously developed oligonucleotide-based, label-free detection methods for nanomolar quantities of Hg 2+ and Ag + ions by employing luminescent platinum(II) metallointercalators ( 34 , 35 ), as well as for assaying exonuclease activity by using crystal violet as a G-quadruplex probe ( 36 ).…”

Section: Introductionmentioning

confidence: 99%

A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaB

Chan

et al. 2011

Nucleic Acids Research

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Transcription factors are involved in a number of important cellular processes. The transcription factor NF-κB has been linked with a number of cancers, autoimmune and inflammatory diseases. As a result, monitoring transcription factors potentially represents a means for the early detection and prevention of diseases. Most methods for transcription factor detection tend to be tedious and laborious and involve complicated sample preparation, and are not practical for routine detection. We describe herein the first label-free luminescence switch-on detection method for transcription factor activity using Exonuclease III and a luminescent ruthenium complex, [Ru(phen)2(dppz)]2+. As a proof of concept for this novel assay, we have designed a double-stranded DNA sequence bearing two NF-κB binding sites. The results show that the luminescence response was proportional to the concentration of the NF-κB subunit p50 present in the sample within a wide concentration range, with a nanomolar detection limit. In the presence of a known NF-κB inhibitor, oridonin, a reduction in the luminescence response of the ruthenium complex was observed. The reduced luminescence response of the ruthenium complex in the presence of small molecule inhibitors allows the assay to be applied to the high-throughput screening of chemical libraries to identify new antagonists of transcription factor DNA binding activity. This will allow the rapid and low cost identification and development of novel scaffolds for the treatment of diseases caused by the deregulation of transcription factor activity.

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Section: Introductionmentioning

confidence: 99%

A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaB

Chan

et al. 2011

Nucleic Acids Research

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“…The binding interactions between metal complexes and nucleic acids have been the subject of numerous studies in recent decades 1 2 3 4 . These interactions are likely to lead to therapeutic (for example, anti-cancer) effects and/or be used for diagnostic (for example, luminescent probes) purposes 5 6 7 8 9 10 11 . To date, the selective targeting of nucleic acids in cancer cells by chemotherapeutic metal complexes in a manner that minimizes off-target bindings and hence diminishes side effects remains a great challenge 12 13 .…”

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confidence: 99%

Luminescent platinum(II) complexes with functionalized N-heterocyclic carbene or diphosphine selectively probe mismatched and abasic DNA

et al. 2016

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The selective targeting of mismatched DNA overexpressed in cancer cells is an appealing strategy in designing cancer diagnosis and therapy protocols. Few luminescent probes that specifically detect intracellular mismatched DNA have been reported. Here we used Pt(II) complexes with luminescence sensitive to subtle changes in the local environment and report several Pt(II) complexes that selectively bind to and identify DNA mismatches. We evaluated the complexes' DNA-binding characteristics by ultraviolet/visible absorption titration, isothermal titration calorimetry, nuclear magnetic resonance and quantum mechanics/molecular mechanics calculations. These Pt(II) complexes show up to 15-fold higher emission intensities upon binding to mismatched DNA over matched DNA and can be utilized for both detecting DNA abasic sites and identifying cancer cells and human tissue samples with different levels of mismatch repair. Our work highlights the potential of luminescent Pt(II) complexes to differentiate between normal cells and cancer cells which generally possess more aberrant DNA structures.

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“…Another possible reason is that, the self-quenching effect of the complexes became less significant after the biological binding. In the presence of BSA, complexes 1b and 2b showed bi-exponential decay, which is not uncommon for biomolecules labeled with luminescent transition metal complexes [43,44]. The binding constants (K a ) and stoichiometries (n) have been determined using the Scatchard equation and the data are summarized in Table 5.…”

Section: Complexmentioning

confidence: 99%

Luminescent cyclometalated iridium(III) dipyridoquinoxaline indole complexes as biological probes

Leung

2010

Sci. China Chem.

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Four luminescent cyclometalated iridium(III) dipyridoquinoxaline complexes appended with an indole moiety [Ir(N^C) 2 (N^N)] (PF 6 ) (HN^C = 2-phenylpyridine, Hppy; (2b)) have been synthesized and characterized. Upon irradiation, all the complexes displayed moderately intense and long-lived luminescence under ambient conditions and in 77 K glass. On the basis of the photophysical data, the emission of the complexes has been assigned to an excited state of triplet metal-to-ligand charge-transfer ( 3 MLCT) ((d(Ir)  *(N^N)) character. Cyclic voltammetric studies revealed indole-based and iridium-based oxidations at ca. +1.10 V and +1.24 V vs. SCE, respectively, and ligand-based reductions at ca. 1.07 to 2.29 V vs. SCE. The interactions of the complexes with an indole-binding protein, bovine serum albumin (BSA), have been examined by emission titrations.binding, indole, iridium, luminescence, probes

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Luminescent Transition Metal Complexes as Biological Labels and Probes

Cited by 41 publications

References 73 publications

A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaB

A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaB

Luminescent platinum(II) complexes with functionalized N-heterocyclic carbene or diphosphine selectively probe mismatched and abasic DNA

Luminescent cyclometalated iridium(III) dipyridoquinoxaline indole complexes as biological probes

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