Luminescent Ru(<scp>ii</scp>)-thiol modified silver nanoparticles for lysosome targeted theranostics

Wumaier, Maierhaba; Yao, Tianming; Hu, Xiaochun; Hu, Zhian; Shi, Shuo

doi:10.1039/c9dt00878k

Cited by 19 publications

(13 citation statements)

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“…This indicated that the casticin-loaded nanoemulsion entered the lysosome after cellular uptake but did not enter the nucleus. Many studies have shown that nanomaterials enter cells through endocytosis to form endosomes and then are accumulated in lysosomes [ 27 ]. Kam et al labeled single-walled carbon nanotubes (SWNT) and co-cultured them with HL60 cells.…”

Section: Resultsmentioning

confidence: 99%

Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate

Zhang

Miao

Huang

et al. 2021

Foods

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Casticin has wide-ranging functional activities, but its water solubility is poor in food products. Here, a nanoemulsion stabilized by Maillard whey protein isolate conjugates (MWPI) was fabricated to encapsulate casticin. The nanoemulsion, with an average diameter of 200 nm, possessed the capability to load 700 μg/g casticin. MWPI-stabilized nanoemulsion showed better stability than that of the WPI nanoemulsion during 4 weeks of storage. Both the inhibition effects of the casticin-loaded nanoemulsion on cancer cells and the process of cellular uptake were studied. Results revealed that the casticin-loaded nanoemulsion had better inhibitory activity in HepG2 and MCF-7 cells than free casticin. Cellular uptake of the nanoemulsion displayed a time-dependent manner. After the nanoemulsion passed into HepG2 and MCF-7 cells, it would locate in the lysosome but not in the nucleus. The main pathway for the nanoemulsion to enter HepG2 cells was pinocytosis, whereas, it entered MCF-7 predominantly through the clathrin-mediated pit. This work implies that MWPI-stabilized nanoemulsions could be utilized as an effective delivery system to load casticin and have the potential to be applied in the food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate

Zhang

Miao

Huang

et al. 2021

Foods

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“…Two of these Ru complexes, Ru32 and Ru33, were also used by Shi and co-workers for functionalization of silver nanoparticles (AgNPs), resulting in monodisperse and homogeneous spherical nanoparticles with an average diameter of 20 nm. [71] AgNPs are known to induce cytotoxicity by an excessive generation of reactive oxygen species (ROS). Functionalization with Ru(II) complexes allowed cell imaging and enhanced anticancer activity.…”

Section: Gold and Silver Nanoparticlesmentioning

confidence: 99%

Incorporation of Ru(II) Polypyridyl Complexes into Nanomaterials for Cancer Therapy and Diagnosis

2020

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Ru(II) metal center. [2] They absorb light and emit wavelength within the red and the near infrared (NIR) spectral regions and possess large Stokes shift, long luminescence lifetime, and potential twophoton (2P) absorption properties. These characteristics have made them highly desirable across numerous research fields such as catalysis, [3] solar energy, [4] sensors [5] and now across the biomedical field either in diagnostics as cellular imaging tools, luminescent probes of DNA structures or in therapy as new classes of anticancer drugs for chemotherapy and photosensitizers (PSs) for photodynamic therapy (PDT) or photoactivated chemotherapy (PACT). [6-11] Worthy of note, a Ru(II) polypyridyl compound (TLD1433) has recently entered phase II clinical trials in Canada for the treatment of nonmuscle invasive bladder cancer as a PS for PDT. [12] However, these complexes still suffer from some drawbacks such as poor water solubility, lack of targeting capability, nonspecific distribution, systemic toxicity and hence, low therapeutic index hampering their translation into the clinic. One strategy to address those medical challenges is to use nanomedicine. Nanomedicine is the application of sub-micron particles (i.e., nanoparticles) in the field of medicine. The materials used for the synthesis and/ or formulation of nanoparticles are extremely diverse, ranging from organic to inorganic molecules (Figure 1). [13] The unique features of nanoparticles including small size, high surface area, surface chemistry, water solubility and multifunctionality make them highly interesting for drug delivery purposes. [14,15] Owing to their size, nanoparticles can passively accumulate to solid tumors while sparing healthy cells thanks to the enhanced permeation and retention (EPR) effect. [16] The EPR effect exploits the abnormalities of tumor vasculature, namely hypervascularity, atypical vascular architecture, leaky vasculature, and lack of lymphatic drainage. This results in the efficient extravasation of nanoparticles from the tumor vasculature and their retention in the tumor interstitium. Therefore, drug incorporation into nanoparticles provides significant improvements in pharmacokinetics, solubility, toxicity, and biodistribution when compared to freely administered molecules, reducing adverse side effects observed with conventional medicine. However, to take full advantage of the EPR effect, nanoparticles must remain in circulation long enough for tumor accumulation. PEGylation, which refers to modification with poly(ethylene glycol) (PEG) chains or PEG copolymers (e.g., Jeffamine), is the most common strategy for imparting stealth properties to nanoparticles. [17] The presence of PEG enables steric stabilization Ru(II) polypyridyl complexes are compounds of great interest in cancer therapy due to their unique photophysical, photochemical, and biological properties. For effective treatment, they must be able to penetrate tumor cells effectively and selectively. The development of nanoscale carriers capable of delivering Ru(I...

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“…[37][38][39] On the contrary, in the passive targeting approach, the nature of the tumor tissue is tackled through their poor lymphatic tissue and leaky, highly permeable vasculature properties, also referred to as the enhanced permeability and retention (EPR) effect, 40 although this concept is currently controversially discussed based to its limitations in in vivo models. 41 Examples involve the encapsulation into polymeric particles, [42][43][44][45][46][47] liposomes [48][49] or functionalization of nanoparticles based on silver, 50 gold, 51 selenium, [52][53] or silica. [54][55][56] Within this context, some attention has been focused on the application of mesoporous silica nanoparticles (MSNs) due to their low price, synthetic accessibility as well as tumor targeting properties.…”

Section: Introductionmentioning

confidence: 99%

“…In the active targeting approach, a specific biological interaction is utilized like signaling peptides, , oligonucleotides, , oligosaccharides, vitamins, , proteins, , antibodies, or receptor targeting moieties. − On the contrary, in the passive targeting approach, the nature of the tumor tissue is tackled through their poor lymphatic tissue and leaky, highly permeable vasculature properties, also referred to as the enhanced permeability and retention (EPR) effect, although this concept is currently controversially discussed based on its limitations in in vivo models . Examples involve the encapsulation into polymeric particles, − liposomes, , or functionalization of nanoparticles based on silver, gold, selenium, , or silica. − …”

Section: Introductionmentioning

confidence: 99%

Ru(II) Polypyridine Complex-Functionalized Mesoporous Silica Nanoparticles as Photosensitizers for Cancer Targeted Photodynamic Therapy

Karges

Díaz-García

Prashar

et al. 2021

ACS Appl. Bio Mater.

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Cancer is the leading cause of death in the developed world. In the last decades, photodynamic therapy (PDT) has augmented the number of medical techniques to treat this disease in the clinics. As the pharmacological active species to kill cancer cells are only generated upon light irradiation, PDT is associated with an intrinsic first level of selectivity. However, since PDT agents are also accumulating in the surrounding, healthy tissue and since it is practically very challenging to only expose the tumor site to light, some side effects can be observed.Consequently, there is a need for a selective drug delivery system, which would give a second level of selectivity. In this work, a dual tumor targeting approach is presented based on mesoporous silica nanoparticles, which act by the enhanced permeability and retention (EPR) effect, and the conjugation to folic acid, which acts as a targeting moiety for folate receptors overexpressed cancer cells. The conjugates were found to be non-toxic in non-cancerous human normal lung fibroblast cells while showing a phototoxic effect upon irradiation at 480 or 540 nm in the low nanomolar range in folate overexpressing cancerous human ovarian carcinoma cells, demonstrating their potential for cancer targeted treatment.

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Luminescent Ru(ii)-thiol modified silver nanoparticles for lysosome targeted theranostics

Abstract: AgNPs modified by Ru(ii) complexes possess bright red fluorescence and may act as potential lysosome-targeted theranostic agents.

Cited by 19 publications

References 50 publications

Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate

Improved Storage Properties and Cellular Uptake of Casticin-Loaded Nanoemulsions Stabilized by Whey Protein-Lactose Conjugate

Incorporation of Ru(II) Polypyridyl Complexes into Nanomaterials for Cancer Therapy and Diagnosis

Ru(II) Polypyridine Complex-Functionalized Mesoporous Silica Nanoparticles as Photosensitizers for Cancer Targeted Photodynamic Therapy

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