Luminescent lanthanide nanoparticle-based imaging enables ultra-sensitive, quantitative and multiplexed <i>in vitro</i> lateral flow immunoassays

Lateral flow assays (LFAs) are currently the most used point-of-care sensors for both diagnostic (e.g., pregnancy test, COVID-19 monitoring) and environmental (e.g., pesticides and bacterial monitoring) applications. Although the core of LFA technology was developed several decades ago, in recent years the integration of novel nanomaterials as signal transducers or receptor immobilization platforms has brought improved analytical capabilities. In this Review, we present how nanomaterial-based LFAs can address the inherent challenges of point-of-care (PoC) diagnostics such as sensitivity enhancement, lowering of detection limits, multiplexing, and quantification of analytes in complex samples. Specifically, we highlight the strategies that can synergistically solve the limitations of current LFAs and that have proven commercial feasibility. Finally, we discuss the barriers toward commercialization and the next generation of LFAs.

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Section: Multiplexingmentioning

confidence: 99%

“…This multiplexed assay was 100 times more sensitive than the comparative AuNP-based LFA, reaching LoDs in the pg mL –1 range for all the biomarkers investigated. Additionally, the researchers developed a portable and cost-effective smartphone-based readout system (see section ; Figure Bii) …”

Section: Multiplexingmentioning

confidence: 99%

Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials

et al. 2022

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“…The maturation stage of these new tools will probably soon open them the doors of commercial applications. In particular, the bio-analytical fields are already tackling the use of Ln-NPs for in vitro applications such as lateral flow immunoassays, 286 ELISA like detection, 287 or luminescence microscopy. 288 However, the high sensitivity obtained with Ln-NPs is based on the development of dedicated instruments, working with a time-delayed detection mode, that will require dedicated time-resolved readers to unravel the best of the sensitivity of these nano-objects.…”

Section: Outlook and Conclusionmentioning

confidence: 99%

Dye-sensitized lanthanide containing nanoparticles for luminescence based applications

et al. 2022

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“…21 Vanadate is considered as an excellent optical entity to enhance the global luminescent emission because of its large absorption cross-sections and effective substrate sensitization band. 22,23 Moreover, Eu 3+ ions in vanadate matrix have been reported to emit strong and stable luminescence. 24 The vanadate NPs doped with Eu 3+ yielded the typical red luminescence of Eu 3+ , which can be excited with near-UV light through an energy transfer from the vanadate anion.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Lanthanide-Doped Nanoparticles-Based Point-of-Care Diagnosis of Human Cardiac Troponin I

Chen¹,

Zhou²,

Zhu³

et al. 2022

IJN

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Cardiac troponin I (cTnI) has been regarded as a gold standard for early diagnosis and prognosis monitoring of acute myocardial infarction (AMI) in clinical practice. Owing to its low concentration in blood, accurate determination of cTnI often requires high sensitivity. However, current established point-of-care (POC) assays are insufficient to meet clinically analytical requirements due to their low sensitivity. Methods: To this end, we established a highly sensitive and reliable POC lateral flow strip based on lanthanide-doped nanoparticles (NPs) for cTnI determination in human blood samples. The capture of cTnI on the lateral flow strip was performed in a sandwich assay, where Eu 3+ -doped vanadate nanoparticles (GdVO 4 :30% Eu NPs) were used as luminescent probes to allow quantification. Results: Our platform realized the analytical sensitivity enhancement with limit-of-detection (LOD) as low as 17 pg mL −1 for cTnI detection, which was lower than the commercial counterpart; meanwhile, it displayed high specificity, excellent reproducibility and outstanding accuracy for analyzing clinical serum samples. Conclusion: Overall, this strategy provided an ultrasensitive, cost-effective and user-friendly platform for on-site cTnI detection, demonstrating the prospect of lanthanide-doped NPs-based POC diagnosis of disease-related biomarkers.

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Luminescent lanthanide nanoparticle-based imaging enables ultra-sensitive, quantitative and multiplexed in vitro lateral flow immunoassays

Abstract: We developed a portable, fast, highly sensitive and quantitative in vitro assay for on-site biomolecule detection by combining the remarkable optical properties of new lanthanide-doped nanoparticle probes with a simple reader coupled to a smartphone.

Cited by 14 publications

References 44 publications

Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials

Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials

Dye-sensitized lanthanide containing nanoparticles for luminescence based applications

Highly Sensitive Lanthanide-Doped Nanoparticles-Based Point-of-Care Diagnosis of Human Cardiac Troponin I

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