Luminescent gelatin nanospheres by encapsulating CdSe quantum dots

Chen, Longyan; Willoughby, A. B.; Zhang, Jin

doi:10.1002/bio.2505

Cited by 18 publications

(12 citation statements)

References 20 publications

(21 reference statements)

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“…37 Moreover, gelatin was also used as the encapsulant of QDs to improve biocompatibility. 38,39 Recently, the fluorescent nanocompsites constructed by assembling CdSe QDs on the exterior of gelatin nanospheres were reported by Chen et al 40 In our paper, gelatin is served as the capping agent, along with TGA. The luminescent AgInS 2 core QDs were first prepared, and their PL intensity was surpassingly enhanced after ZnS coating.…”

Section: Introductionmentioning

confidence: 93%

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS₂/ZnS Core/Shell Quantum Dots

et al. 2015

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Approximately 3 g of water-soluble AgInS 2 /ZnS core/shell quantum dots (AIS/ZnS QDs) with a maximum photoluminescence quantum yield of up to 39.1% was synthesized in an aqueous solution of gelatin and thioglycolic acid (TGA). The composition of the AIS QDs could be readily adjusted by controlling the molar ratio of the starting Ag/In precursors in the reaction solution, which leads to a tunable emission ranging from 535 to 607 nm. The as-prepared core/shell QDs exhibit excellent photostability and water/buffer stability. More importantly, these cadmium-free hydrophilic AIS/ZnS core/shell QDs are biocompatible and can be directly utilized in cancer cell imaging.

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Section: Introductionmentioning

confidence: 93%

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS₂/ZnS Core/Shell Quantum Dots

et al. 2015

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“…This finding suggests the QDs covalently bind into the GNs through the reaction between the amine groups of cysteamine modified on the QDs and the carboxylic groups on the exterior of the GNs. Due to the covalent bonding, the hybridization performed post nanoparticle synthesis will not affect the drug loading process nor contribute to the release of QDs as seen in the method described by Chen et al [27].…”

Section: Resultsmentioning

confidence: 99%

Engineering large gelatin nanospheres coated with quantum dots for targeted delivery of human osteosarcoma with enhanced cellular internalization

Tse

Gyenis

Litchfield

et al. 2014

14th IEEE International Conference on Nanotechnology

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Due to the special structures of cell membrane, good internalization is one of major concerns on using large nanospheres as carriers for labeling and treatment of cancer. We herein report fluorescent gelatin nanosphers (GNs) coated with CdSe/ZnS quantum dots (QDs) to form a viable vehicle for theranostic applications. Anti-human immunoglobulin G Fab formation, anti-IgG Fab, is bioconjugated on to the hybrid fluorescent GNs for targeted delivery (QDs-GNs-anti-IgG Fab). Human osteosarcoma cell line is used in studying the interaction between hybrid fluorescent GNs with and without anti-IgG Fab. The average particle size of the fluorescent GNs bioconjugated with antibodies is estimated at 480±50 nm. The emission (λem) of the fluorescent GNs is around 652 nm. The quantitative analysis on the surface modification and bioconjugation of GNs has been discussed in this paper. The three-dimensional zstacking fluorescent images reveal that the hybrid GNs with anti-IgG Fab has ~1.5 times increase in internalization with human osteosarcoma cells than GNs without the antibody fragment. The improved cellular interaction of the QDs-GN-anti IgG Fab is attributed to the bioconjugation of antibodies which can provide specificity for targeted drug delivery. Relative cell viability (%) is larger than 80% for each type of functionalized GNs up to 40 μg/mL. We expect that the functionalized gelatin sphere can offer an effective theranostic tool for targeted drug delivery and direct imaging confirmation simultaneously.. 978-1-4799-5622-7/$31.00 ©2014 IEEE

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“…44 Our previous results indicated that no significant toxic effect is imposed on cells when the concentration of GNs is up to 5 mg/mL. 45 With further increasing the concentration of MUA-QDs up to 6.25 mg/mL, the cell viability dramatically decreases to 20%. Meanwhile, the relative cell viability (%) drops to 50% when the concentration of QDs-GNs increases up to 25 mg/mL, which might be related to the negative interference of fluorescent detection caused by the high concentration of nontransparent QDs-GNs.…”

Section: ■ Experimental Sectionmentioning

confidence: 97%

Development of Biocompatible and Proton-Resistant Quantum Dots Assembled on Gelatin Nanospheres

Chen

Siemiarczuk²,

Hai

et al. 2014

Langmuir

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In this study, biocompatible and proton-resistant CdSe quantum dots (QDs) assembled on gelatin nanospheres (GNs) have been synthesized by combining the two-step desolvation method with the layer-by-layer assembly technique. The core-shell fluorescent gelatin nanosphere consists of a gelatin core and a four-layer shell of hydrophilic CdSe QDs assembled through polyelectrolytes (PE). The morphology, microstructures, and photostability of the hybrid spheres were further investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorospectrometery, and confocal fluorescent microscopy (CFM), respectively. The average diameter of the hybrid QDs-gelatin nanospheres (QDs-GNs) is estimated at 484 ± 40 nm. Our results indicate that the 20 ± 5 nm of the shell is attributed to the four-layer of CdSe QDs assembled through the PE. QD-GNs show a strong photoluminescence with the maximum emission (λ(em)) at 613 nm at the excitation wavelength of 470 nm. The core-shell QDs-GNs are able to resist quenching in acidic solution (pH < 4). Furthermore, core-shell QDs-GNs show a longer lifetime in a broad range of pH values, from 9 to 1. The calculated average lifetime (τ(ave)) of QDs-GNs is about 889 ± 23 ps, which is 3-fold longer than that of MUA-QDs (263 ± 10 ps) at pH 7.0. The enhanced lifetime of QDs-GNs is almost 9 times of that of CdSe QDs when pH value is 1. Meanwhile, the cell viability study shows that no significant toxic effect is imposed on the NIH/3T3 mouse fibroblast cell line when the concentration of QD-GNs is below 5 mg/mL. It is expected that this new biocompatible fluorescent nanospheres could be an excellent alternative fluorescent imaging agent for cell labeling, especially in acidic conditions.

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Luminescent gelatin nanospheres by encapsulating CdSe quantum dots

Cited by 18 publications

References 20 publications

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS₂/ZnS Core/Shell Quantum Dots

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS₂/ZnS Core/Shell Quantum Dots

Engineering large gelatin nanospheres coated with quantum dots for targeted delivery of human osteosarcoma with enhanced cellular internalization

Development of Biocompatible and Proton-Resistant Quantum Dots Assembled on Gelatin Nanospheres

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Luminescent gelatin nanospheres by encapsulating CdSe quantum dots

Cited by 18 publications

References 20 publications

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS2/ZnS Core/Shell Quantum Dots

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS2/ZnS Core/Shell Quantum Dots

Engineering large gelatin nanospheres coated with quantum dots for targeted delivery of human osteosarcoma with enhanced cellular internalization

Development of Biocompatible and Proton-Resistant Quantum Dots Assembled on Gelatin Nanospheres

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Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS₂/ZnS Core/Shell Quantum Dots

Scaling up the Aqueous Synthesis of Visible Light Emitting Multinary AgInS₂/ZnS Core/Shell Quantum Dots