Luminescent/colorimetric probes and (chemo-) sensors for detecting anions based on transition and lanthanide ion receptor/binding complexes

Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells

“…Indeed, previously reported synthetic receptors have shown limited discrimination between ATP and ADP at enzyme relevant concentrations of Mg 2+ ions . We showed recently that [ Eu.1 ] + can discriminate effectively between ATP and ADP in a buffered aqueous solution containing 3 m m Mg 2+ ions: adding 1 m m ADP caused an 8‐fold increase in overall Eu III emission intensity compared to a much smaller 2.5‐fold intensity increase in the presence of 1 m m ATP . Excellent discrimination between ATP and ADP was attributed to the slightly higher affinity of [ Eu.1 ] + for ADP (Table ) as well as the higher competition between Mg 2+ and ATP compared to ADP.…”

“…[26,37] We showed recently that [Eu.1] + + can discriminate effectively betweenA TP and ADP in ab uffered aqueous solution containing 3mm Mg 2 + ions: adding 1mm ADP caused an 8-fold increasei no verall Eu III emissioni ntensity compared to am uch smaller2 .5-fold intensity increase in the presenceo f1m m ATP. [24] Excellent discrimination betweenA TP andA DP was attributed to the slightly highera ffinity of [Eu.1] + for ADP (Table 1) 2À .T itrationso fA DP and AMP into an aqueous solution containing [Eu.3] + and 5mm Mg 2 + ions resulted in standard hyperbolic curves that were fitted to a1 :1 binding model ( Figure S56 and S57). Apparentassociation constants weredetermined to be logK a = 4.6 and 3.8 for ADP and AMP respectively,a pproximately 1o rder of magnitude lower than those determinedi nt he absence of MgCl 2 (Table 2).…”

“…Certain Eu III and Tb III complexes have been shown to bind reversibly to anions in aqueous media, including bicarbonate, fluoride, lactate , phosphate and phosphorylated peptides . Anion binding can be signalled by changes in the intensity ratio of two Ln III emission bands or by changes in emission lifetime of the complex . However, examples of Ln III complexes that bind to NPP anions such as ATP are rare .…”

“…[23] Anion binding can be signalled by changes in the intensity ratio of two Ln III emission bands or by changes in emission lifetimeo f the complex. [13,24] However, examples of Ln III complexes that bind to NPP anions such as ATPa re rare. [25][26][27][28] Bindingt oA TP is usually weaka nd causes quenching of luminescence due to energy transfer to the nucleotide base [25,27] or displacement of acoordinated sensitizing ligand from the Ln III ion (decomplexation).…”

Mix‐&‐Read Determination of Mercury(II) at Trace Levels with Hybrid Mesoporous Silica Materials Incorporating Fluorescent Probes by a Simple Mix‐&‐Load Technique

“…Finally, the simple mix‐&‐read test‐strip assay demonstrated that this approach is very appealing for powerful and reliable analytics for everyday life in a relevant concentration range. If one considers that over the past four decades a vast number of small‐molecule probes for a large number of analytes have been reported in the literature, of which, however, only a few operate in neat aqueous solution, reconsideration of one or the other probe molecules and combination with suitably tailored nanomaterials in view of our present work might lead in a rather straightforward manner to various other potent indicator materials for comparatively simple analytical applications.…”