Luminescence of telomeric fragments of DNA macromolecule

Yashchuk, Valeriy M.; Kudrya, Vladislav Yu.; Dubey, I. Ya.; Kovalyuk, Kateryna I.; Batsmanova, O.I.; Mel'nik, V. I.; Klishevich, G. V.

doi:10.1080/15421406.2016.1255068

Cited by 2 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescence spectral bands of (-)PBS and (+)PBS at = 78 K (Fig. 2) are close to each other, as well as to the band of oligunucleotide Tel-22 investigated by us earlier [5][6][7]. They do not co- Fig.…”

Section: Fluorescencesupporting

confidence: 81%

“…The difference between them ( Fig. 1, line 3, "difference") at = 295 nm is likely associated with the existence of G-complexes (possibly, G-quadruplexes or G-intermediates) [5,6]. As for (+)PBS, the optical absorption spectrum of (-)PBS differs to some extent from the equimolar sum of the corresponding spectra of deoxyribonucleotides (in the ratio A : G : C : T = 1 : 6 : 7 : 4) that are in the composition of (-)PBS oligonucleotide.…”

Section: Optical Absorptionmentioning

confidence: 99%

“…As for (+)PBS, the optical absorption spectrum of (-)PBS differs to some extent from the equimolar sum of the corresponding spectra of deoxyribonucleotides (in the ratio A : G : C : T = 1 : 6 : 7 : 4) that are in the composition of (-)PBS oligonucleotide. The difference between them (the band at = 295 nm) is associated with the existence of G-complexes too [5,6]. The "difference" bands were obtained by the subtraction of the equimolar sums from the corresponding spectra of oligonucleotides.…”

Section: Optical Absorptionmentioning

confidence: 99%

“…The difference between (-)PBS/ (+)PBS and dAMP phosphorescence spectra (the band at = 530 nm) is thought to be associated with an unknown center, possibly, G-complex or G-intermediate that dominates in the fluorescence. Thus, the main centers of triplet excitation capturing in (-)PBS and (+)PBS oligonucleotides are A-bases and some centers of an unknown nature (possibly, G-complexes or G-intermediates, but they are not AT-complexes observed by us in [3][4][5][6][7]). The "1-3-difference" band was obtained by the subtraction of the dAMP spectral band from the (-)PBS spectral band.…”

Section: Phosphorescencementioning

confidence: 99%

“…The first main goal of our investigations is the identification of the absorbing, fluorescent and phosphorescent centers in (-)PBS and (+)PBS. On the other hand, it is known [3][4][5][6][7] that the capturing centers of the triplet excitation in DNA are AT-sequences. Recently, we showed that this AT-complex is formed by neighbor A-and T-chromophores from the same strand (not from A-and T-complementary chromophores of the different strands) [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Spectral Properties of Single-Stranded Viral DNA Fragment

et al. 2018

View full text Add to dashboard Cite

This article presented the results of investigations of the optical absorption (at 300 K) and steady-state and time-resolved luminescence (at 78 K) of (–)PBS and (+)PBS oligonucleotides. (–)PBS is the DNA form of the minus primer binding site (5′GTCCCTGTTCGGGCGCCA3′) of the human immunodeficiency virus type 1 (HIV-1)genome, and (+)PBS (3′CAGGGACAAGCCCGCGGT5′) is its complementary sequence [1]. The optical absorption spectra of (–)PBS and (+)PBS do not coincide with the correspondent equimolar sums of the spectra of nucleotides that are in their composition. The difference between them at 295 nm is related to the existence of some stable complex between bases (possibly, G-complexes). The fluorescence spectral bands of (–)PBS and (+)PBS are close to each other and to the band of oligonucleotide investigated by us in [2]. In our opinion, the (–)PBS and (+)PBS bands are connected possibly with the fluorescence of some complexes that are manifested in the absorption. The phosphorescence spectral bands of (–)PBS and (+)PBS are close to each other and to the band of dAMP (in the wavelength interval 370–470 nm). The difference between the (–)PBS/(+)PBS and dAMP phosphorescence spectra (at 530 nm) is associated with an unknown center (possibly, G-complexes). Thus, the main centers of the triplet excitation capturing in (–)PBS and (+)PBS are A-bases and centers of an unknown nature.

show abstract

Section: Fluorescencesupporting

confidence: 81%

Section: Optical Absorptionmentioning

confidence: 99%

Section: Optical Absorptionmentioning

confidence: 99%

Section: Phosphorescencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Spectral Properties of Single-Stranded Viral DNA Fragment

et al. 2018

View full text Add to dashboard Cite

show abstract

The spectral properties of DNA and RNA macromolecules at low temperatures: fundamental and applied aspects

Yashchuk

Kudrya

2017

Methods Appl. Fluoresc.

View full text Add to dashboard Cite

This paper summarizes the results of studies of the spectral properties-optical absorption, fluorescence and phosphorescence-of DNA and RNA macromolecules and synthetic poly-, oligo- and mono-nucleotides, which have been carried out in our laboratory. The system of first excited singlet and triplet energy levels for DNA and RNA is evaluated using low-temperature (4.2 K-77 K) luminescent measurements. The traps of the singlet and triplet electronic excitations in these compounds are identified. An important self-protection mechanism against photo-damage of DNA and RNA by UV photons or penetrative radiation based on the capture of triplet electronic-energy excitations by the most photostable centers-in DNA, the complex formed by neighboring adenosine (A) and thymidine (T) links; in RNA, the adenosine links-is described. It is confirmed that despite similarities in the chemical and partly energy structures DNA is more stable than RNA. The spectral manifestation of the telomeres (the important functional system) in DNA macromolecules is examined. The results obtained on telomere fragments provide the possibility of finding the configuration peculiarities of the triplet excitations traps in DNA macromolecules. The resulting spreading length of the migrating singlet (l ) and triplet (l) excitations for DNA and RNA macromolecules are evaluated.

show abstract

Luminescence of telomeric fragments of DNA macromolecule

Cited by 2 publications

References 27 publications

Spectral Properties of Single-Stranded Viral DNA Fragment

Spectral Properties of Single-Stranded Viral DNA Fragment

The spectral properties of DNA and RNA macromolecules at low temperatures: fundamental and applied aspects

Contact Info

Product

Resources

About