Luminal Antioxidants Enhance the Effects of Mesalamine in the Treatment of Chemically Induced Colitis in Rats

Abstract

Global assessments of microscopic colitis induced by TNBS indicated that 5-ASA alone significantly changed colonic injury by -31%. Combination therapy with ascorbic acid plus 5-ASA or alpha-tocopherol plus 5-ASA caused further significant change in TNBS colitis by -65 and -82%, respectively. Each of these values was significantly below scores observed with 5-ASA as monotherapy. Reduction in colitis with PBN plus 5-ASA was not different from 5-ASA alone. MPO activity was decreased significantly in response to monotherapy with 5-ASA and each of the antioxidants plus 5-ASA when compared to TNBS. alpha-Tocopherol plus 5-ASA, however, was the only treatment strategy that reduced significantly MPO activity below that recorded for 5-ASA alone. In conclusion, our results indicate that antioxidants other than N-acetylcysteine significantly enhance the therapeutic effectiveness of 5-ASA in the treatment of TNBS colitis. alpha-Tocopherol plus 5-ASA exerted profound anti-inflammatory and reparative effects upon colitis induced by TNBS.

5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

“…Studies also demonstrate that combination of 5-ASA with other antioxidants such as n-acetylcysteine produces better clinical and histological responses when compared with 5-ASA alone, suggesting that the capacity of reducing ROS in tissues is proportional to the increased antioxidant activity [53][54][55][56]. By such actions, 5-ASA can decrease or completely inhibit the oxidative DNA damage known to be involved in pathogenesis of UC, DC, and colitis-associated CRC [25,43,[58][59][60][61]. Although 5-ASA has been used in patients with UC, few studies have evaluated its therapeutic effects in patients with DC [40,41].…”

“…The importance of using antioxidants in treatment of DC is underscored by clinical study results demonstrating that 5-aminosalicylic acid (5-ASA) was equally effective in treatment of both ulcerative colitis (UC) and DC [40][41][42][43][44][45]. However, the effectiveness of 5-ASA in protecting against oxidative tissue damage in experimental models of DC has never been studied.…”

Comparison of anti-inflammatory activities of an anthocyanin-rich fraction from Portuguese blueberries (Vaccinium corymbosum L.) and 5-aminosalicylic acid in a TNBS-induced colitis rat model

“…The dose of ARF used was chosen on basis of preliminary experiments, as the lowest to obtain safety and efficacy in the referred colitis rat model, and that of 5-ASA was as reported in literature [5,27] and taking into consideration the dose translation from animal to humans [28]. …”

Comparative Protective Effect of Hawthorn Berry Hydroalcoholic Extract, Atorvastatin, and Mesalamine on Experimentally Induced Colitis in Rats

“…Previous studies showed the MPO activity elevation in both AA-and trinitrobenzenesulfonic acid-induced experimental colitis in rodents. 16,30 The second part of the current study was devoted to examine the gastroprotective effect of Crataegus monogyna berry extract and atorvastatin on the AA-induced colitis. Moreover, any possible interaction between test compounds was also investigated.…”