Lumican effectively regulates the estrogen receptors-associated functional properties of breast cancer cells, expression of matrix effectors and epithelial-to-mesenchymal transition

Καραμάνου, Κωνσταντίνα; Franchi, Marco; Piperigkou, Zoi; Perreau, Corinne; Maquart, François‐Xavier; Vynios, Demitrios H.; Brézillon, Stéphane

doi:10.1038/srep45138

Cited by 60 publications

(50 citation statements)

References 42 publications

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“…MDA-MB-231 human cells express this triple-negative immunoprofile and are the main cell line used to investigate this breast cancer subtype 5 , 6 . They are flattened and spindle-like cells with increased cell-cell contacts and an aggressive phenotype 7 . Moreover, the 4T1 murine cells are also typical triple negative breast cancer cell line and closely mimic human breast cancer considering characteristics as anatomical site, immunogenicity and growth development.…”

Section: Introductionmentioning

confidence: 99%

Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells

Pizato

Luzete

Kiffer

et al. 2018

Sci Rep

176

113

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The implication of inflammation in pathophysiology of several type of cancers has been under intense investigation. Omega-3 fatty acids can modulate inflammation and present anticancer effects, promoting cancer cell death. Pyroptosis is an inflammation related cell death and so far, the function of docosahexaenoic acid (DHA) in pyroptosis cell death has not been described. This study investigated the role of DHA in triggering pyroptosis activation in breast cancer cells. MDA-MB-231 breast cancer cells were supplemented with DHA and inflammation cell death was analyzed. DHA-treated breast cancer cells triggered increased caspase-1and gasdermin D activation, enhanced IL-1β secretion, translocated HMGB1 towards the cytoplasm, and membrane pore formation when compared to untreated cells, suggesting DHA induces pyroptosis programmed cell death in breast cancer cells. Moreover, caspase-1 inhibitor (YVAD) could protect breast cancer cells from DHA-induced pyroptotic cell death. In addition, membrane pore formation showed to be a lysosomal damage and ROS formation-depended event in breast cancer cells. DHA triggered pyroptosis cell death in MDA-MB-231by activating several pyroptosis markers in these cells. This is the first study that shows the effect of DHA triggering pyroptosis programmed cell death in breast cancer cells and it could improve the understanding of the omega-3 supplementation during breast cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells

Pizato

Luzete

Kiffer

et al. 2018

Sci Rep

176

113

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“…In cancer, lumican attenuates the proliferation, migration, and invasion of breast cancer cells. It modifies cellular junctions and promotes MET 131 through direct interactions with other ECM molecules or by the modulation of membrane receptors 132,133 and MMP-14. [134][135][136] Glypicans are proteoglycans that participate in developmental morphogenesis.…”

Section: Epithelial-mesenchymal Transition: What Is New?mentioning

confidence: 99%

Molecular principles of metastasis: a hallmark of cancer revisited

Fares

Khachfe

et al. 2020

Sig Transduct Target Ther

1,409

935

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Metastasis is the hallmark of cancer that is responsible for the greatest number of cancer-related deaths. Yet, it remains poorly understood. The continuous evolution of cancer biology research and the emergence of new paradigms in the study of metastasis have revealed some of the molecular underpinnings of this dissemination process. The invading tumor cell, on its way to the target site, interacts with other proteins and cells. Recognition of these interactions improved the understanding of some of the biological principles of the metastatic cell that govern its mobility and plasticity. Communication with the tumor microenvironment allows invading cancer cells to overcome stromal challenges, settle, and colonize. These characteristics of cancer cells are driven by genetic and epigenetic modifications within the tumor cell itself and its microenvironment. Establishing the biological mechanisms of the metastatic process is crucial in finding open therapeutic windows for successful interventions. In this review, the authors explore the recent advancements in the field of metastasis and highlight the latest insights that contribute to shaping this hallmark of cancer.

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“…Decorin (DCN), biglycan (BGN), fibromodulin (FMO), and lumican (LUM) are the best-characterized members of the SLRP family and have been extensively studied. DCN was found to inhibit downstream oncogenic signaling in several solid tumors by binding and antagonizing various receptor tyrosine kinases [ 18 ]; LUM was reported to have anticancer activity by effectively regulating the estrogen receptor-associated functional properties of breast cancer cells, the expression of matrix effectors, and the epithelial-to-mesenchymal transition [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of mechanical stretching on the morphology of extracellular polymers and the mRNA expression of collagens and small leucine-rich repeat proteoglycans in vaginal fibroblasts from women with pelvic organ prolapse

et al. 2018

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To determine the effect of mechanical stretching load and the efficacy of postmenopausal estrogen therapy (ET) on pelvic organ prolapse (POP), vaginal fibroblasts isolated from postmenopausal women with or without POP were subjected to 0.1-Hz uniaxial cyclic mechanical stretching (CS) with 10% elongation and 10−8 M 17-β-estradiol (E2) treatment. We investigated the morphological characteristics of extracellular polymers using scanning electron microscopy (SEM) and monitored the mRNA expression of type I collagen (COL I) and type III collagen (COL III) as well as the small leucine-rich proteoglycan (SLRP) family members decorin (DCN), biglycan (BGN), fibromodulin (FMO), and lumican (LUM), using real-time quantitative polymerase chain reaction (RT-PCR). Using SEM, certain viscoelastic polymers were found to be randomly distributed among fibroblasts, which for normal fibroblasts formed clusters of plum flower-like patterns under static-culture conditions and resembled stretched strips when stretched in culture, whereas polymers among POP fibroblasts resembled stretched strips under static-cultured conditions and presented broken networks when stretched in culture. RT-PCR revealed that COL I, DCN, BGN, FMO, and LUM mRNA expression was significantly higher in POP than in normal fibroblasts under static-culture condition. Following CS, COL I and BGN mRNA expression was significantly up-regulated in normal fibroblasts, and DCN and FMO mRNA expression was down-regulated in POP fibroblasts. Following concomitant CS and E2 treatment, significantly elevated COL I and DCN mRNA expression was observed in normal fibroblasts, and significantly elevated COL I and BGN mRNA expression was observed in POP fibroblasts. COL III mRNA expression was not significantly different between the POP and normal group, and CS did not significantly affect expression in either group, though COL III was down-regulated in normal fibroblasts concomitantly treated with E2 and CS. We conclude that the morphological distribution of extracellular polymers in POP fibroblasts exhibited higher sensitivity and lower tolerance to stretching loads than do normal fibroblasts. These mechanical properties were further reflected in the transcription of COL I. Defects in the compensatory function of BGN for DCN and LUM for FMO exist in POP fibroblasts, which further affect the structure and function of COL I in response to stretching load, ultimately resulting in abnormal reconstruction of pelvic supportive connective tissues and the occurrence of POP. ET can maintain stretching-induced elevations in COL I and DCN transcription in healthy women and improve stretching-induced COL I, DCN, BGN, and FMO transcriptional changes in POP women to prevent and improve POP. Only down-regulated COL III transcription was observed upon concomitant CS and E2 treatment in normal fibroblasts, which suggests that the tensile strength, not the elasticity, of the supportive connective tissues is damaged in POP and that the higher tensile strength induced by ET in healthy fibroblasts ...

show abstract

Lumican effectively regulates the estrogen receptors-associated functional properties of breast cancer cells, expression of matrix effectors and epithelial-to-mesenchymal transition

Cited by 60 publications

References 42 publications

Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells

Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells

Molecular principles of metastasis: a hallmark of cancer revisited

Effects of mechanical stretching on the morphology of extracellular polymers and the mRNA expression of collagens and small leucine-rich repeat proteoglycans in vaginal fibroblasts from women with pelvic organ prolapse

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