2011
DOI: 10.1091/mbc.e10-06-0554
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Lumenal interactions in nuclear pore complex assembly and stability

Abstract: A mechanism of nuclear pore complex assembly into intact nuclear envelopes remains elusive. We explore roles of conserved inner nuclear membrane proteins, Heh1p and Heh2p, in this process. The data support the existence of a lumenal bridge between Heh1p and the nucleoporin Pom152p, which might facilitate early nuclear pore complex assembly events.

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Cited by 57 publications
(76 citation statements)
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“…Since nups are found in multiple copies in a mature NPC, NPC assembly requires at least 450 nup protomers in yeast (Alber et al, 2007a; 2007b) and many more in human cells (Bui et al, 2013). The spatiotemporal regulation of these events and the biochemical intermediates in this process remain ill defined, although integral INM proteins of the LEM (Lap2-emerin-MAN1)(Yewdell et al, 2011) and SUN (Sad1, UNC84) families (Talamas and Hetzer, 2011) might help form early NPC assembly intermediates.…”
Section: Introductionmentioning
confidence: 99%
“…Since nups are found in multiple copies in a mature NPC, NPC assembly requires at least 450 nup protomers in yeast (Alber et al, 2007a; 2007b) and many more in human cells (Bui et al, 2013). The spatiotemporal regulation of these events and the biochemical intermediates in this process remain ill defined, although integral INM proteins of the LEM (Lap2-emerin-MAN1)(Yewdell et al, 2011) and SUN (Sad1, UNC84) families (Talamas and Hetzer, 2011) might help form early NPC assembly intermediates.…”
Section: Introductionmentioning
confidence: 99%
“…2, will then drive the shape transformation of the nucleus by overcoming the elevated bending and surface energies stored in the deformed membrane. Recent evidence has indeed shown that various inner nuclear membrane proteins such as Heh1, Heh2, and Ima1 can bind and anchor chromatids to the NE (28)(29)(30)(31). Thus, it is conceivable that such connection prevents chromosomes from sliding away from the nuclear membrane during their contact and ultimately allows the poleward force to be transmitted to the NE.…”
Section: Theoretical Modelmentioning
confidence: 99%
“…In a surveillance model, Heh2 and/or ESCRT-III would have the capacity to recognize these aberrant assembly intermediates to facilitate their removal. Since Heh2 is capable of binding nups, but is not a stable component of NPCs, 29 we think that it is the most likely candidate that might help biochemically distinguish between an NPC assembly intermediate and an NPC. The mechanism by which Heh2 might perform this function is not obvious, but we favor a model in which there is an upstream factor likely in the ubiquitinproteasome system capable of recognizing and ubiquitylating aberrant NPC assembly intermediates.…”
Section: Escrt-iii Prevents Aberrant Npc Assemblymentioning
confidence: 99%
“…For example, several studies suggest the involvement of integral INM proteins in helping to form an early NPC assembly intermediate. 29,38 Moreover, NLSs are found on nups important for early assembly events. [39][40][41][42] Lastly, biochemical intermediates and invaginations of the INM have been observed after the genetic imposition of assembly blocks.…”
Section: Does Escrt-iii Directly Contribute To Npc Biogenesis?mentioning
confidence: 99%