Lumbar body fusion with a bioresorbable cage in a goat model is delayed by the use of a carboxymethylcellulose‐stabilized collagenous rhOP‐1 device

Mullender, Margriet G.; Krijnen, Matthijs R.; Helder, Marco N.; Smit, Theo H.; Everts, Vincent; Wuisman, P.

doi:10.1002/jor.20285

Cited by 7 publications

(6 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CMC and HPMC are considered suitable carriers based on evidence that both substances support the osteoinductive capacities of BMP‐25, 21–27 and BMP‐7,6–10, 28 as well as the osteoconductive properties of bone substitutes 29. However, whether CMC or HPMC can control the formation or the activity of osteoclasts and osteoblasts is unknown.…”

Section: Discussionmentioning

confidence: 99%

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Agis

Beirer

Watzek

et al. 2010

J Biomedical Materials Res

View full text Add to dashboard Cite

Carboxymethylcellulose (CMC) and hydroxypropylmethylcellulose (HPMC) serve as carriers for growth factors and bone substitutes. Although both carriers are placed into the defect sites, their impacts on bone regeneration are unclear. Herein, we examined whether CMC and HPMC affect the differentiation of bone marrow progenitors into osteoclasts and osteoblasts. We therefore induced osteoclastogenesis and osteoblastogenesis in murine bone marrow progenitors in the presence of CMC and HPMC, respectively. Measures of osteoclastogesis were based on the number and activity of tartrate-resistant acid-phosphatase-positive (TRAP(+)) multinucleated cells and expression of marker genes. Osteoblastogenesis was determined by the number and activity of alkaline-phosphatase-positive (AP(+)) colonies and relevant marker genes. Viability was assessed by colorimetric measurement of formazan formation. We report that CMC at 1% caused a significant reduction in the number and activity of TRAP(+) multinucleated cells. Changes in viability were not responsible for the observed effects. HPMC showed no remarkable impact on osteoclastogenesis; however, the concentration was limited to 0.5% because of the high viscosity. The ability of bone marrow progenitors to form AP(+) colonies was not affected by either of the two carriers. Together, these results suggest that CMC and possibly also HPMC can decrease osteoclastogenesis while osteoblastogenesis remains unchanged in vitro. These observations raise the possibility that these carriers might affect the cellular process of bone regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Agis

Beirer

Watzek

et al. 2010

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Many studies have reported the successful use of goat models to study the spine 192021. Therefore, although the results cannot be directly extrapolated to humans the experimental results obtained may play an important role in guiding human lumbar anterior surgery.…”

Section: Discussionmentioning

confidence: 99%

Large blood vessel stretch in lumbar spine through anterior surgical approach: An experimental study in adult goat

Liu

Wang

Zhou

et al. 2014

IJOO

View full text Add to dashboard Cite

Background:Various anterior lumbar surgical approaches, including the minimally invasive approach, have greatly improved in recent years. Vascular complications resulting from ALIF are frequently reported. Little information is available about the safety of large blood vessel stretch. We evaluated the right side stretch limit (RSSL) of the abdominal aorta (AAA) and the inferior vena cava (IVC) without blood flow occlusion and investigated stretch-induced histological injury and thrombosis in the iliac and femoral arteries and veins and the stretched vessels.Materials and Methods:The RSSL of blood vessels in five adult goats was measured by counting the number of 0.5-cm-thick wood slabs that were inserted between the right lumbar edge and the stretch hook. Twenty seven adult goats were divided into three groups to investigate histological injury and thrombosis under a stretch to 0.5 cm (group I) 1.5 cm (group II) for 2 h, or no stretch (group III). Blood vessel samples from groups I and II were analyzed on postsurgical days 1, 3, and 7. Thrombogenesis was examined in the iliac and femoral arteries and veins.Results:The RSSL of large blood vessels in front of L4/5 was 1.5 cm from the right lumbar edge. All goats survived surgery without complications. No injury or thrombosis in the large blood vessels in front of the lumbar vertebrae and in the iliac or femoral arteries and veins was observed. Under light microscopy, group I showed slight swelling of endothelial cells in the AAA and no histological injury of the IVC. The AAA of group II showed endothelial cell damage, unclear organelles, and incomplete cell connections by electron microscopy.Conclusions:The AAA and IVC in a goat model can be stretched by ≤0.5 cm, with no thrombosis in the AAA, IVC, iliac or femoral arteries and veins.

show abstract

“…80 This is likely due to the putty component of the BMP-7 preparation, which recruits multinucleated giant cells. 113 Bone morphogenetic protein-2 BMP-2 is a potent osteoinductive molecule that has been shown to increase and speed up osteogenic differentiation, and induce healing of intrabony periodontal defects as well as critical size defects in animals. 82,86,105,114 BMP-2 applied to osteoprogenitor cells 5,115,116 and/or to osteoconductive scaffolds 22,43,76,[81][82][83][84][85][86]105,114 stimulates osteogenic differentiation in vitro and accelerates bone healing and new bone formation in vivo (Table 3).…”

Section: Bone Morphogenetic Protein-7mentioning

confidence: 99%

Human Maxillary Sinus Floor Elevation as a Model for Bone Regeneration Enabling the Application of One-Step Surgical Procedures

Farré-Guasch

Prins

Overman

et al. 2013

Tissue Engineering Part B: Reviews

Self Cite

View full text Add to dashboard Cite

Bone loss in the oral and maxillofacial region caused by trauma, tumors, congenital disorders, or degenerative diseases is a health care problem worldwide. To restore (reconstruct) these bone defects, human or animal bone grafts or alloplastic (synthetic) materials have been used. However, several disadvantages are associated with bone graft transplantation, such as limited bone volume, donor-site morbidity, surgical and immune rejection risks, and lack of osseo-integration. Bone tissue engineering is emerging as a valid alternative to treat bone defects allowing the regeneration of lost bony tissue, thereby recovering its functionality. During the last decades, the increasing aged population worldwide has also raised the prevalence of maxillary atrophy. Maxillary sinus floor elevation (MSFE) has become a standard surgical procedure to overcome the reduced amount of bone, thus enabling the placement of dental implants. MSFE aims to increase the bone height in the posterior maxilla, by elevating the Schneiderian membrane and placing the graft material into the surgically created space in the maxillary sinus floor. Importantly, oral bone regeneration during MSFE offers a unique human clinical model in which new cell-based bone tissue engineering applications might be investigated, since biopsies can be taken after MSFE before a dental implant placement and analyzed at the cellular level. New approaches in oral bone regeneration are focusing on cells, growth factors, and biomaterials. Recently, adipose tissue has become interesting as an abundant source of mesenchymal stem cells, which might be applied immediately after isolation to the patient allowing a one-step surgical procedure, thereby avoiding expensive cell culture procedures and another surgical operation. In this new cell-based tissue engineering approach, stem cells are combined with an osteoconductive scaffold and growth factors, and applied immediately to the patient. In this review, MSFE is discussed as a valid model to test bone tissue engineering approaches, such as the one-step surgical procedure. This procedure might be applied in other regenerative medicine applications as well.

show abstract

Lumbar body fusion with a bioresorbable cage in a goat model is delayed by the use of a carboxymethylcellulose‐stabilized collagenous rhOP‐1 device

Cited by 7 publications

References 44 publications

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Large blood vessel stretch in lumbar spine through anterior surgical approach: An experimental study in adult goat

Human Maxillary Sinus Floor Elevation as a Model for Bone Regeneration Enabling the Application of One-Step Surgical Procedures

Contact Info

Product

Resources

About