Lull pgm system: A suitable technique to improve the regenerative potential of autologous fat grafting

Abstract

Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, that is, the decantation and Coleman's centrifugation techniques. The lowest number of erythrocytes, which are proinflammatory cells, was observed in the Lull samples, corresponding to about 50% of those isolated by decantation and centrifugation. The highest amount of SVF cells were isolated from the Lull samples whose number of colony forming units, representative of the amount of adipose-derived stem cells (ADSCs), was about fourfold and sixfold higher than in the decantation and centrifugation samples, respectively. Adipocyte and osteoblast commitment of SVF cells obtained from all the three procedures also confirmed that the subpopulation of ADSCs was actively represented in the processed aspirates. Moreover, the growth rate of the SVF cells was more accentuated in the samples obtained from decantation and Lull than centrifugation. In conclusion, Lull seems to be the best processing technique for adipose tissue graft with respect to decantation and centrifugation, because it clears more efficiently the fat from proinflammatory blood cells and provides the greatest number of proliferating SFV cells and ADSCs.

The composition of cell-based therapies obtained from point-of-care devices/systems which mechanically dissociate lipoaspirate: a scoping review of the literature

“…Most of the included studies were low level evidence (Table 2) [12, 16–18, 20, 24, 27, 29, 42, 61, 63, 65, 66, 75, 77] 11 were Level 5 [12, 16, 17, 20, 27, 42, 61, 63, 66, 75, 77] (descriptive laboratory studies), one was Level 4 [18] and only three were Level 2 [24, 29, 65].…”

“…All included studies [12, 16–18, 20, 24, 27, 29, 42, 61, 63, 65, 66, 75, 77] disclosed whether they had any financial or other competing interests. 73.3% ( n = 11/15 [12, 17, 20, 24, 27, 29, 42, 61, 63, 66, 77]) gave a clear objective which reduced the risk of outcome bias.…”

Optimizing Fat Grafting Using a Hydraulic System Technique for Fat Processing: A Time and Cost Analysis

“…Therefore, the graft should be gently washed and/or contaminates should be further removed through centrifugation. Recently Morselli et al 15 19 and Zhu et al 20 reported two closed systems for fat washing with decantation and centrifugation, showing that their procedures achieved the best balance between purification and amount of stem cells.…”

“…Since our study is an evolution of the fat washing technique proposed by Morselli et al, it was deemed unnecessary to demonstrate the quality and survival rate of the transferred fat. Indeed, Morselli et al 15 19 has already outlined that his technique rendered good fat survival rates. Decantation caused no harm to mature adipocytes and preserved an elevated amount of ADSCs among all the SVF.…”

“…Tedde's system, described by Rubino et al 14 in 2014, was used as the first step of our technique (tumescent solution's infiltration) and then for fat washing. The Lull System by Morselli et al, 15 represented the main principle for our fat washing, the second step of the standard system ( Fig. 1A ) and our Module B .…”