Lucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells

Akbaş, Fahri; Özaydın, Ahmet; Polat, Erdal; Onaran, İlhan

doi:10.25135/rnp.180.20.03.1582

Cited by 9 publications

(18 citation statements)

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“…Generally, the cell migration plays an essential role in wound repairing. The ES components such as serine proteases promote the cell migration of fibroblast 45–47 and enhance the tissue formation. 48 Actually, as mentioned in literature, 49 ES enhanced the wound healing activity of novel antibacterial drugs (sulfonamide derivatives).…”

Section: Resultsmentioning

confidence: 99%

Wound healing and antibacterial activities of water-soluble chitosan nanoparticles and excretion/secretion as a natural combination from medicinal maggots, Lucilia cuprina

Abdel-Samad

Taher

2021

Journal of Bioactive and Compatible Polymers

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Wounds management takes a high interest in the medical field and the addition of antimicrobial agents in an assortment of wound dressings leads to delay the wound healing. This study aimed at preparing natural combination between excretion/secretion (ES) and water-soluble chitosan nanoparticles (from Lucilia cuprina maggots) and investigating its antibacterial and wound healing activities. ES of maggots was collected, and the water-soluble chitosan nanoparticles (WSCNPs) were prepared and characterized. Antibacterial activities of combinations were evaluated against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Proteus vulgaris. ES-WSC-2 combination that contains 50% ES and 1% WSCNPs showed highest antibacterial activity against all tested bacteria compared to the other combinations. In vitro, the ES-WSC-2 combination was used to study the wound healing activity by scratch assay. The synergism between ES and WSCNPs (in ES-WSC-2 combination) accelerated the wound healing rate which suggests the use of this combination as an effective natural antibacterial and wound healing agent.

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Section: Resultsmentioning

confidence: 99%

Wound healing and antibacterial activities of water-soluble chitosan nanoparticles and excretion/secretion as a natural combination from medicinal maggots, Lucilia cuprina

Abdel-Samad

Taher

2021

Journal of Bioactive and Compatible Polymers

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“…The results of this study showed that the reduction of the wound surface in each group was significant from the first day (P≤0.001) and wound size changes in the E/S group with the negative control group have a significant difference (P≤0.011). In another study, It was found L.sericata larval secretions could modulate the mRNA expression of some wound healing-related genes and contains components that are effective in wound healing (Akbas et al 2020). The most important aid for healing burn wounds is wound bed preparation, which has different methods .The standard method for dealing with such wounds is fast and wide debridement with a graft (Madihally et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in experimental scalding burn injury

et al. 2020

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Abstract. Amiri ZS, Akbarzadeh K, Douraghi M, Abdi KM, Afshar AA, Ghaffari J, Kordshouli RS, Akbari M, Rafinejad J. 2021. Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in an experimental scalding burn injury. Nusantara Bioscience 13: 11-15. Lucilia sericata larvae have been successfully used as medicinal larvae for wound healing. This study was performed to evaluate the therapeutic effects of the primary ointment made from excretory-secretory substances of L.sericata larvae on experimental burn wounds on rabbits under laboratory conditions. Extraction of ES from the third stage of L.sericata larvae and antibiogram testing was done. Four rabbits were randomly divided into four groups; three standard third-grade burn wounds were created on the back of each rabbit for intervention groups (B, D) of wounds were used respectively from the original ointment made from ES and ES without accompanying material. Nitrofurazone ointment was used for the positive (C) and placebo ointment was used for the negative (A). On the 21st day, all scars of wounds in groups B and D were separated and the wound was free of infectious tissue, whereas in groups A and C that the scars were clinging to the base. The level of the ulcer was significantly lower in the E/S intervention group and the infectious tissue was not observed during the treatment of group B and D wounds. Methicillin-resistant Staphylococcus aureus (MRSA) did not show resistance to excretory-secretory substances of L. sericata larvae. The debridement and recovery process was faster in the ES-treated group and the initial ointment than in the control group. The results showed that maggot extractions and secretion (E/S) of L. sericata in reducing wound healing is appropriate for treatment.

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“…The best ionization of small and relatively polar compounds was obtained by ESI source. The ions between m/z 85-1500 were scanned in high-resolution mode of instrument [11,[54][55][56][57][58][59][60][61]. Identification of compounds was performed by comparison of retention time of standard compounds (in the range of purity 95-99% see section chemicals) and HRMS data of Bezmialem Vakif University, Drug Application and Research Center Library (ILMER).…”

Section: Optimization Of Hplc Methods and Lc-hrms Proceduresmentioning

confidence: 99%

“…The concentration of final solution (1 mL) was added in a capped autosampler vial, from which 2 µL of sample was injected to LC for each run. The prepared samples in the auto sampler were stored at 15 o C [53][54][55][56][57].…”

Section: Preparation Of Samples For Lc-hrms Analysismentioning

confidence: 99%

Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

Kızıltaş¹,

Gören²,

Bingöl³

et al. 2021

Rec.Nat.Prod.

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To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

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Lucilia sericata Larval Secretions Stimulating Wound Healing Effects on Rat Dermal Fibroblast Cells

Cited by 9 publications

References 0 publications

Wound healing and antibacterial activities of water-soluble chitosan nanoparticles and excretion/secretion as a natural combination from medicinal maggots, Lucilia cuprina

Wound healing and antibacterial activities of water-soluble chitosan nanoparticles and excretion/secretion as a natural combination from medicinal maggots, Lucilia cuprina

Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in experimental scalding burn injury

Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

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