Lubricin in human temporomandibular joint disc: An immunohistochemical study

Leonardi, Rosalia; Musumeci, Giuseppe; Sicurezza, Edoardo; Loreto, Carla

doi:10.1016/j.archoralbio.2011.12.004

Cited by 23 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As the central region of the disc bears the majority of frictional forces during normal mandibular movement, these results appear to correspond with what others have seen in three-dimensional chondrocyte scaffolds, with increasing PRG4 expression in response to mechanical stimuli (39)(40)(41). Our observation of intense PRG4 staining in disc cells agrees with the distribution of PRG4 found in human TMJ discs with (18 discs) and without (34 discs) internal derangement; PRG4 was more abundant in the surface region of discs than in the deeper, central regions (42,43). Enrichment of PRG4 on the disc surface is consistent with the observations that mechanical stimuli promote PRG4 expression, indicating that PRG4 plays a lubricating role at the site of articulation between the surfaces of the disc and the cranial base, and the disc and the mandible.…”

Section: Discussionsupporting

confidence: 90%

Transcriptional regulation of proteoglycan 4 by 17β‐estradiol in immortalized baboon temporomandibular joint disc cells

McDaniel

Babu

Navarro

et al. 2014

European J Oral Sciences

View full text Add to dashboard Cite

Temporomandibular joint disorders (TMDs) affect a significant portion of the U.S. population with the majority seeking treatment being women of childbearing age. Due to this striking sexual dimorphism it has been postulated that there is a role for sex hormones in the maintenance of normal temporomandibular joint (TMJ) function. Proteoglycan 4 (PRG4) is a secreted lubricating molecule required for maintaining low frictional levels within articular joints, however its role in the TMJ is not well characterized. In this study we describe the development of immortalized baboon cells isolated from specific regions of the TMJ disc and their use in the investigation of PRG4 expression and localization patterns in the TMJ. We identified conserved estrogen response elements within the 5′ flanking region of the PRG4 gene of several species, and found that treatment of baboon TMJ disc cells with estrogen led to reduced PRG4 promoter activity and mRNA expression in vitro. The observed negative regulation of PRG4 by estrogen could lead to increased friction and degradation of joint components over time. This study, for the first time, provides evidence of the regulatory potential of estrogen on PRG4 gene expression and suggests a novel etiology for the gender disparity observed among TMD patients.

show abstract

Section: Discussionsupporting

confidence: 90%

Transcriptional regulation of proteoglycan 4 by 17β‐estradiol in immortalized baboon temporomandibular joint disc cells

McDaniel

Babu

Navarro

et al. 2014

European J Oral Sciences

View full text Add to dashboard Cite

show abstract

“…These complex functional demands result in a heterogenous tissue with differences in the regional distribution of extracellular matrices and cell phenotypes. Thus the mature disc has a fibrous tendinous structure with fibroblastic cells at sites of tension, a primarily cartilaginous matrix and chondrocyte-like cells at sites of compression and a mixed tissue composition and intermediate fibrochondrocytic cells where the tissue is subjected to complex tensile, compressive and shear forces 12,20,23-27 . Ultimately, this structural organization and composition of the disc is determined by the cells contained within the tissue.…”

Section: Introductionmentioning

confidence: 99%

Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation

Park

Hosomichi

et al. 2015

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

Objective Despite the importance of TMJ disc in normal function and disease, studying the responses of its cells has been complicated by the lack of adequate characterization of the cell subtypes. The purpose of our investigation was to immortalize, clone, characterize and determine the multi-lineage potential of mouse TMJ disc cells. Design Cells from 12-week-old female mice were cultured and immortalized by stable transfection with human telomerase reverse transcriptase. The immortalized cell clones were phenotyped for fibroblast- or chondrocyte-like characteristics and ability to undergo adipocytic, osteoblastic and chondrocytic differentiation. Results Of 36 isolated clones, four demonstrated successful immortalization and maintenance of stable protein expression for up to 50 passages. Two clones each were initially characterized as fibroblast-like and chondrocyte-like on the basis of cell morphology and growth rate. Further the chondrocyte-like clones had higher mRNA expression levels of cartilage oligomeric matrix protein (>3.5-fold), collagen × (>11-fold), collagen II expression (2-fold) and collagen II:I ratio than the fibroblast-like clones. In contrast, the fibroblast-like clones had higher mRNA expression level of vimentin (>1.5-fold), and fibroblastic specific protein 1 (>2.5-fold) than he chondrocyte-like clones. Both cell types retained multi-lineage potential as demonstrated by their capacity to undergo robust adipogenic, osteogenic and chondrogenic differentiation. Conclusions These studies are the first to immortalize TMJ disc cells and characterize chondrocyte-like and fibroblast-like clones with retained multi-differentiation potential that would be a valuable resource in studies to dissect the behavior of specific cell types in health and disease and for tissue engineering.

show abstract

“…Lubricin is a chondroprotective glycoprotein that serves as a critical boundary lubricant between opposing cartilage surfaces (Leonardi et al., ). It has a major protective role in preventing cartilage wear and synovial cell adhesion, proliferation, and in reducing the coefficient of friction of the articular cartilage surface (Leonardi et al., 2012b, c). Several recent studies found that treatment with recombinant lubricin could protect articular cartilage and prevent the process of OA in animal models (Teeple et al., ).…”

mentioning

confidence: 99%

Physical activity ameliorates cartilage degeneration in a rat model of aging: A study on lubricin expression

Musumeci

Castrogiovanni

Trovato

et al. 2014

Scandinavian Med Sci Sports

111

View full text Add to dashboard Cite

Osteoarthritis (OA) is a common musculoskeletal disorder characterized by slow progression and joint tissue degeneration. Aging is one of the most prominent risk factors for the development and progression of OA. OA is not, however, an inevitable consequence of aging and age-related changes in the joint can be distinguished from those that are the result of joint injury or inflammatory disease. The question that remains is whether OA can be prevented by undertaking regular physical activity. Would moderate physical activity in the elderly cartilage (and lubricin expression) comparable to a sedentary healthy adult? In this study we used physical exercise in healthy young, adult, and aged rats to evaluate the expression of lubricin as a novel biomarker of chondrocyte senescence. Immunohistochemistry and western blotting were used to evaluate the expression of lubricin in articular cartilage, while enzyme-linked immunosorbent assay was used to quantify lubricin in synovial fluid. Morphological evaluation was done by histology to monitor possible tissue alterations. Our data suggest that moderate physical activity and normal mechanical joint loading in elderly rats improve tribology and lubricative properties of articular cartilage, promoting lubricin synthesis and its elevation in synovial fluid, thus preventing cartilage degradation compared with unexercised adult rats.

show abstract

Lubricin in human temporomandibular joint disc: An immunohistochemical study

Cited by 23 publications

References 31 publications

Transcriptional regulation of proteoglycan 4 by 17β‐estradiol in immortalized baboon temporomandibular joint disc cells

Transcriptional regulation of proteoglycan 4 by 17β‐estradiol in immortalized baboon temporomandibular joint disc cells

Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation

Physical activity ameliorates cartilage degeneration in a rat model of aging: A study on lubricin expression

Contact Info

Product

Resources

About