Lubricin: A versatile, biological anti-adhesive with properties comparable to polyethylene glycol

Greene, George W.; Martin, Lisandra L.; Tabor, Richard Francis; Michalczyk, Agnes; Ackland, M. Leigh; Horn, Roger G.

doi:10.1016/j.biomaterials.2015.02.086

Cited by 87 publications

(172 citation statements)

References 44 publications

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“…Complete characterization of LUB's self‐assembly, self‐assembled structure, and antiadhesive properties have been previously carried out and are described in prior studies. QCM measurements quantifying LUB self‐assembly on gold surfaces together with AFM imaging of the LUB layer surface morphology can be found in ref.…”

Section: Resultsmentioning

confidence: 99%

“…QCM measurements quantifying LUB self‐assembly on gold surfaces together with AFM imaging of the LUB layer surface morphology can be found in ref. and so have not been duplicated for this study. In the following sections, we describe the results of cyclic voltammetry experiments performed using uncoated and LUB coated gold electrodes for the oxidation/reduction of a 3.6 × 10 −3 m solution of K 3 [Fe(CN) 6 ] redox couple prepared in nonfouling PBS solution, fouling PBS solution containing 10 mg mL −1 BSA, and a 50% dilution of blood plasma in PBS.…”

Section: Resultsmentioning

confidence: 99%

“…PRG4, is a glycoprotein found in the synovial fluid and covering the cartilage surfaces of articular joints whose important role as a major boundary lubricant in articular joints is now well known . In addition to being a biological lubricant, recent experiments have demonstrated that LUB exhibits impressive antiadhesive properties that are as‐good‐as and, in some cases, better than chemically grafted layers of polyethylene glycol . LUB's antiadhesive properties, ability to self‐assemble into a well‐ordered “telechelic” polymer brush layer on virtually any substrate (including gold), and over‐abundance of anionic charge density make it an attractive, multifunctional coating material in technologies such as microfluidics and biosensors.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to being a biological lubricant, recent experiments have demonstrated that LUB exhibits impressive antiadhesive properties that are as‐good‐as and, in some cases, better than chemically grafted layers of polyethylene glycol . LUB's antiadhesive properties, ability to self‐assemble into a well‐ordered “telechelic” polymer brush layer on virtually any substrate (including gold), and over‐abundance of anionic charge density make it an attractive, multifunctional coating material in technologies such as microfluidics and biosensors. Greene et al have recently demonstrated that LUB coatings can be quickly and easily applied to silica, PTFE, and PDMS‐based microfluidic devices to virtually eliminate all nonspecific adsorption of proteins to the device surfaces .…”

Section: Introductionmentioning

confidence: 99%

“…The majority of the LUB molecule's length is derived from the heavy glycosylated central portion that is commonly referred to as the “mucin domain.” Within this mucin domain, short glycan oligomers terminated primarily by polar galactose (≈33% of total glycans) and negatively charged sialic acid (≈66% of total glycans) are O‐linked to threonine and serine residues . This central mucin domain is responsible for LUB's excellent lubrication and antiadhesive properties . Flanking either end of the central mucin domain are two globular and highly adhesive “end domains,” which contain subdomains similar to two proteins, somatomedin‐B and hemopexin, known also to be present in extracellular adhesion proteins such as vitronectin .…”

Section: Introductionmentioning

confidence: 99%

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Lubricin Antiadhesive Coatings Exhibit Size‐Selective Transport Properties that Inhibit Biofouling of Electrode Surfaces with Minimal Loss in Electrochemical Activity

Greene

Ortiz

Pozo‐Gonzalo

et al. 2018

Adv Materials Inter

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Electrochemical sensing is a highly effective technique for rapidly detecting and quantifying the presence of electroactive compounds in solution. However, effective sensing requires the electrode surface to be free of contamination, which makes this technique particularly susceptible to the effects of surface fouling due to the unwanted adhesion of biomolecules. Unfortunately, coating electrodes with antiadhesive molecules typically causes electrical passivation with detrimental effects upon electrochemical processes. Lubricin is a large glycoprotein found in articular joints, which self‐assembles on most substrates and exhibits excellent antiadhesive properties. This report finds that lubricin antiadhesive coatings function as a size‐selective transport barrier that physically blocks large, fouling molecules (e.g., proteins) while still allowing small molecules to diffuse into, out‐of, and within this layer more‐or‐less unimpeded. The diffuse nature of the lubricin coatings ensures the electrode retains a large, “free” surface area that facilitates efficient charge transfer. Using cyclic voltammetry to measure the oxidation/reduction of a K3[Fe(CN)6] redox couple at a gold electrode (a system particularly susceptible to fouling), this report shows that antiadhesive lubricin brush coatings can be used for highly sensitive amperometric/voltametric detection of small electroactive compounds in highly fouling solutions of proteins and blood plasma with minimal, immediate impact upon the electrochemistry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Lubricin Antiadhesive Coatings Exhibit Size‐Selective Transport Properties that Inhibit Biofouling of Electrode Surfaces with Minimal Loss in Electrochemical Activity

Greene

Ortiz

Pozo‐Gonzalo

et al. 2018

Adv Materials Inter

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Mussel‐Inspired Hydrogels for Self‐Adhesive Bioelectronics

Xie

Wang

et al. 2020

Adv Funct Materials

335

240

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Wearable and implantable bioelectronics are receiving a great deal of attention because they offer huge promise in personalized healthcare. Currently available bioelectronics generally rely on external aids to form an attachment to the human body, which leads to unstable performance in practical applications. Self‐adhesive bioelectronics are highly desirable for ameliorating these concerns by offering reliable and conformal contact with tissue, and stability and fidelity in the signal detection. However, achieving adequate and long‐term self‐adhesion to soft and wet biological tissues has been a daunting challenge. Recently, mussel‐inspired hydrogels have emerged as promising candidates for the design of self‐adhesive bioelectronics. In addition to self‐adhesiveness, the mussel‐inspired chemistry offers a unique pathway for integrating multiple functional properties to all‐in‐one bioelectronic devices, which have great implications for healthcare applications. In this report, the recent progress in the area of mussel‐inspired self‐adhesive bioelectronics is highlighted by specifically discussing: 1) adhesion mechanism of mussels, 2) mussel‐inspired hydrogels with long‐term and repeatable adhesion, 3) the recent advance in development of hydrogel bioelectronics by reconciling self‐adhesiveness and additional properties including conductivity, toughness, transparency, self‐healing, antibacterial properties, and tolerance to extreme environment, and 4) the challenges and prospects for the future design of the mussel‐inspired self‐adhesive bioelectronics.

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Recent Developments and Practical Feasibility of Polymer‐Based Antifouling Coatings

Maan

Hofman

Vos

et al. 2020

Adv Funct Materials

423

343

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While nature has optimized its antifouling strategies over millions of years, synthetic antifouling coatings have not yet reached technological maturity. For an antifouling coating to become technically feasible, it should fulfill many requirements: high effectiveness, long‐term stability, durability, ecofriendliness, large‐scale applicability, and more. It is therefore not surprising that the search for the perfect antifouling coating has been going on for decades. With the discovery of metal‐based antifouling paints in the 1970s, fouling was thought to be a problem of the past, yet its untargeted toxicity led to serious ecological concern, and its use became prohibited. As a response, research shifted focus toward a biocompatible alternative: polymer‐based antifouling coatings. This has resulted in numerous advanced and innovative antifouling strategies, including fouling‐resistant, fouling‐release, and fouling‐degrading coatings. Here, these novel and exciting discoveries are highlighted while simultaneously assessing their antifouling performance and practical feasibility.

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Lubricin: A versatile, biological anti-adhesive with properties comparable to polyethylene glycol

Cited by 87 publications

References 44 publications

Lubricin Antiadhesive Coatings Exhibit Size‐Selective Transport Properties that Inhibit Biofouling of Electrode Surfaces with Minimal Loss in Electrochemical Activity

Lubricin Antiadhesive Coatings Exhibit Size‐Selective Transport Properties that Inhibit Biofouling of Electrode Surfaces with Minimal Loss in Electrochemical Activity

Mussel‐Inspired Hydrogels for Self‐Adhesive Bioelectronics

Recent Developments and Practical Feasibility of Polymer‐Based Antifouling Coatings

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