LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane

Shingote, Prashant Raghunath; Mithra, S. V. Amitha; Sharma, Pratima; Devanna, N.; Arora, Kirti; Holkar, Somnath Kadappa; Khan, Mohammad Suhail; Singh, Jogendra; Kumar, Sanjeev; Sharma, Tilak Raj; Solanke, Amolkumar U.

doi:10.1007/s11032-019-0931-5

Cited by 11 publications

(18 citation statements)

References 31 publications

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“…The plant in vitro cultures are of interest for the production of secondary metabolites of commercial interest (Loyola-Vargas and Ochoa-Alejo 2018). Plants obtained via PTC were used in genetic studies to generate mapping population for dissection of QTLs linked to agronomic traits (Tyrka et al 2018) and in studies of mobile elements (Shingote et al 2019). For a long time, in vitro culture methods were considered as the way of generating plants that are identical to the donor plants (Metakovsky et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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The in vitro tissue cultures are, beyond all difficulties, an essential tool in basic research as well as in commercial applications. Numerous works devoted to plant tissue cultures proved how important this part of the plant science is. Despite half a century of research on the issue of obtaining plants in in vitro cultures, many aspects remain unknown. The path associated with the reprogramming of explants in the fully functioning regenerants includes a series of processes that may result in the appearance of morphological, physiological, biochemical or, finally, genetic and epigenetic changes. All these changes occurring at the tissue culture stage and appearing in regenerants as tissue culture-induced variation and then inherited by generative progeny as somaclonal variation may be the result of oxidative stress, which works at the step of explant preparation, and in tissue culture as a result of nutrient components and environmental factors. In this review, we describe the current status of understanding the genetic and epigenetic changes that occur during tissue culture. Key message Variation appeared in regenerated plants as well as variation inherited by generative progeny of regenerants can may many, positive or negative impact, of gained plant materials. This review focused on factors that triggered this phenomenon with underlying oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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“…They reported that both methods produced highly polymorphic bands in samples. Shingote et al (2019) also identified that IRAP system was superior to ISSR in terms of marker in terms of index, resolving power and polymorphic loci per assay.…”

Section: Irap (Inter Retrotransposon Amplified Polymorphism)mentioning

confidence: 96%

Application of different molecular markers in biotechnology

Kocaman

Toy

Marakli

2020

International Journal of Science Letters

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“…Numerical Taxonomy System (NTSYS-pc) ver. 2.10e was used to construct dendrograms and similarity matrices [22]. The Jaccard's similarity coefficient between pair of accessions was then calculated using the binary data matrix via the SIMQUAL model [22].…”

Section: Cluster Analysismentioning

confidence: 99%

IRAPs in Combination with Highly Informative ISSRs Confer Effective Potentials for Genetic Diversity and Fidelity Assessment in Rhododendron

Wen¹,

Zhao²,

Zhang³

et al. 2023

Preprint

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Rhododendron is well-known for the colorful corolla. Molecular marker systems have the potentials to elucidate genetic diversity as well as to assess the genetic fidelity in rhododendrons. In the present study, the reverse transcription domains of long terminal repeat retrotransposons were cloned from rhododendrons and used to develop inter-retrotransposon amplified polymorphism (IRAP) marker system. Subsequently, 198 polymorphic loci were generated from the IRAP and inter-simple sequence repeat (ISSR) markers, of which 119 were derived from the IRAP markers. It was justified that in rhododendrons, IRAP markers were superior to the ISSRs in some polymorphic parameters such as the average number of polymorphic loci (14.88 versus 13.17). In comparison with the single one, the combination of IRAPs and ISSRs systems was more discriminative for detecting 46 rhododendron accessions. Further, IRAP markers demonstrated more efficiency in genetic fidelity detection of in vitro R. bailiens, an endangered species just recorded in Guizhzhou Province, China. The available evidences revealed the distinct properties of IRAP and ISSR markers in the rhododendron-associated applications, and highlighted the availability of highly informative ISSR and IRAP markers in the genetic diversity evaluation and the genetic fidelity assessment of rhododendrons, which may facilitate the preservation and genetic breeding in rhododendron plants.

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LTR retrotransposons and highly informative ISSRs in combination are potential markers for genetic fidelity testing of tissue culture-raised plants in sugarcane

Cited by 11 publications

References 31 publications

Plant tissue culture environment as a switch-key of (epi)genetic changes

Plant tissue culture environment as a switch-key of (epi)genetic changes

Application of different molecular markers in biotechnology

IRAPs in Combination with Highly Informative ISSRs Confer Effective Potentials for Genetic Diversity and Fidelity Assessment in Rhododendron

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