<title>The effect of low-intensity laser therapy on wound healing in Streptozotocin-induced diabetic rats</title>

Abstract

Diabetes Mellitus is a condition that results in a delay of the wound healing process, that is associated with an insufficient production of collagen, a decrease of the amount of collagen fibrils and deficient blood flow in the wound area. It is suggested that Low Intensity Laser Therapy acts by improving wound healing in normal organisms, accelerating tissue regeneration. The aim of this work was to investigate the biostimulatory effect of the HeNe laser irradiation, at 632.8 nm, on wound healing in 15 male rats suffering from diabetes induced by Streptozotocin, compared to 15 control diabetic animals. Irradiation parameters were: laser power of 15mW, exposition time of 17 s., irradiated area ofO.025 cm2 and laser energy density of 10 J/cm2. Full-thickness skin squared samples, with 5 mm of non-injured tissue around the wound, were obtained at 4, 7 and 15 days after wounding procedure (5 treated and 5 control animals each time). The histopathologic analysis performed by haematoxylin-eosin staining. Results suggested that the irradiation of diabetic rats was efficient for wound healing. Treated group presented better quality of the wound tissues by the macroscopic observation than control group and the microscopic analysis demonstrated that treated animals had better histopathologic evaluation than non treated.

Effective Combination Therapy with Human Amniotic Membrane Mesenchymal Stem Cells and Low-Level Laser Therapy in Wound Healing in Animal Models

“…9,10 Some studies investigating the laser as an alternative or adjunctive method for surgical wounds have shown its efficacy in enhancing surgical closure, reducing pain and infection, and shortening the period of wound healing. [11][12][13] Different parameters and irradiation conditions like exposure time, frequency, and duration of treatment influence the treatment outcome. 14 Therefore, it is necessary that researchers recognize the appropriate selection of these parameters in different studies to develop a successful treatment.…”