&lt;title&gt;Real-time atomic force fluorescence microscopy on living cells&lt;/title&gt;

Struckmeier, Jens; Klopp, Erk; Born, Matthias; Hofmann, Martin R.; Rink, Damaris; Jones, David B.; Ecke, Stefan; Butt, Hans‐Jürgen

doi:10.1117/12.446671

Cited by 1 publication

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References 13 publications

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“…An atomic-force microscope (AFM) with a small ball at the top of the cantilever as described previously (Struckmeier et al, 2001) was used to press on the polyacrylamide sheets. The total indentation caused by the ball fixed to the cantilever was between 0.5 µm and 1.5 µm.…”

Section: Methodsmentioning

confidence: 99%

Dynamic changes in traction forces with DC electric field in osteoblast-like cells

Curtze

Dembo

Miron

et al. 2004

Journal of Cell Science

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Primary bovine osteoblasts and human osteosarcoma cells exposed to direct-current electric fields undergo processes of retraction and elongation ultimately resulting in the realignment of the long cellular axis perpendicular to the electric field. The time taken for this reorientation was inversely correlated to field strength within a certain range. Cellular force output during reorientation was analyzed using a simple modification of traction force microscopy. The first detectable reaction was an increase in average traction force magnitude occurring between 10 and 30 seconds of electric field exposure. In the following 2 to 15 minutes traction forces at margins tangential to the electric field decreased below their initial values. Phase-contrast microscopy revealed elongating protrusions at these margins several minutes later. We could not correlate the initial traction changes with any change in intracellular free calcium levels measured using the fluorescent dye Fura-2 AM.

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Section: Methodsmentioning

confidence: 99%