&lt;title&gt;Raman spectra of crystalline 4Zn, 2Zn, and Na insulin&lt;/title&gt;

Tensmeyer, Lowell G.; Shields, James E.

doi:10.1117/12.22913

Cited by 7 publications

(3 citation statements)

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“…The Raman features of the human insulin spectra, (a−c) in Figure , correspond closely to previously reported normal Raman spectra attributed to insulin in its native conformational state (and detailed band assignments are given in ref a). Not surprisingly, spectra taken on the ring, (b) and (c), have a substantially greater signal-to-noise ratio than those taken in the interior of the DCDR deposition area, away from the ring (a), where the intensity of insulin Raman features are smaller and there is a higher broadband background intensity.…”

Section: Resultssupporting

confidence: 84%

Raman Detection of Proteomic Analytes

et al. 2003

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The compatibility of nonenhanced Raman spectroscopy with chromatographic and mass spectroscopic proteomic sensing is demonstrated for the first time. High-quality normal Raman spectra are derived from protein solutions with concentrations down to 1 microM and 1 fmol of protein nondestructively probed within the excitation laser beam. These results are obtained using a drop coating deposition Raman (DCDR) method in which the solution of interest is microdeposited (or microprinted) on a compatible substrate, followed by solvent evaporation and backscattering detection. Representative applications include the DCDR detection of insulin derived from an HPLC fraction, nondestructive DCDR followed by MALDI-TOF of lysozyme, the DCDR detection of protein spots deposited using an ink-jet microprinter, and the identification of spectral differences between glycan isomers of equal mass (such as those derived from posttranslationally modified proteins).

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Section: Resultssupporting

confidence: 84%

Raman Detection of Proteomic Analytes

et al. 2003

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“…From Fig. 5b it can be seen that for all formulation the S-S vibration bands are located close to 513 cm -1 suggesting that all disulphide bonds are in an adopted more stable gauche -gauchegauche conformation [47,48] as a result of the complete water removal during inkjet printing.…”

Section: Raman Spectroscopymentioning

confidence: 93%

3D printed microneedle patches using stereolithography (SLA) for intradermal insulin delivery

Economidou

Pere

Reid

et al. 2019

Materials Science and Engineering: C

215

113

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3D printed microneedle arrays were fabricated using a biocompatible resin through stereolithography (SLA) for transdermal insulin delivery. Microneedles were built by polymerising consecutive layers of a photopolymer resin. Thin layers of insulin and sugar alcohol or disaccharide carriers were formed on the needle surface by inkjet printing. The optimization of the printing process resulted in superior skin penetration capacity of the 3D printed microneedles compared to metal arrays with minimum applied forces varying within the range of 2 to 5N. Micro-CT analysis showed strong adhesion of the coated films on the microneedle surface even after penetration to the skin. In vivo animal trials revealed fast insulin action with excellent hypoglycaemia control and lower glucose levels achieved within 60 min, combined with steady state plasma glucose over 4 h compared to subcutaneous injections.

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“…Other crystalline forms of human insulin (e.g., 4Zn, 2Zn, Zn free) have also been analyzed using Raman [20]. In the current work, DCDR spectra of insulin variants in the native conformational state are obtained after purification by reverse phase high-performance liquid chromatography (RP-HPLC).…”

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confidence: 99%