&lt;title&gt;Improvement of cancer PDT using sulphophthalocyanine and sodium ascorbate&lt;/title&gt;

Kalija, Oleg L.; Meerovich, Gennadii A.; Torshina, Nadezgda L.; Коган, Е. А.; Loschenov, Victor B.; Luk’yanets, E. A.; Kogan, Boris; Butenin, A. V.; Vorozhtsov, Georgy N.; Kuzmin, Sergey G.; Volkova, Anna I.; Posypanova, Anna M.

doi:10.1117/12.297800

Cited by 4 publications

(4 citation statements)

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“…Some of these techniques have the potential for operating at the level of single cells [1][2][3][4] . In these methods, only cancer cells are specifically targeted based on their abnormality, while normal cells are unaffected.…”

Section: Introductionmentioning

confidence: 99%

Photothermal ablation therapy for cancer based on metal nanostructures

Rozanova

Zhang

2009

Sci. China Ser. B-Chem.

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Besides conventional surgery, radiation therapy, and chemotherapy, which all tend to have side-effects and damage normal tissues, new medical strategies, such as photothermal sensitization and photothermal ablation therapy (PTA) with near-IR laser light, have been explored for treating cancer. Much of the current excitement surrounding nanoscience is directly connected to the promise of new nanotechnology for cancer diagnosis and therapy. The basic principle behind PTA is that heat generated from light can be used to destroy cancer cells. Strong optical absorption and high efficiency of photothermal conversion at the cancer sites are critical to the success of PTA. Because of their unique optical properties, e.g., strong surface plasmon resonance (SPR) absorption, noble metal nanomaterials, such as gold and silver, have been found to significantly enhance photothermal conversion for PTA applications. Substantial effort has been made to develop metal nanostructures with optimal structural and photothermal properties. Ideal metal nanostructures should have strong and tunable SPR, be easy to deliver, have low toxicity, and be convenient for bioconjugation for actively targeting specific cancer cells. This review would highlight some gold nanostructures with various shapes and properties, including nanoparticles (NPs), nanorods (NRs), nanoshells, nanocages, and hollow nanospheres, which have been studied for PTA applications. Among these structures, hollow gold nanospheres (HGNs) exhibit arguably the best combined properties because of their small size (30-50 nm), spherical shape, and strong, narrow, and tunable SPR absorption.

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Section: Introductionmentioning

confidence: 99%

Photothermal ablation therapy for cancer based on metal nanostructures

Rozanova

Zhang

2009

Sci. China Ser. B-Chem.

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“…For example, ascorbate enhanced phthalocyanine-sensitized photo hemolysis of human erythrocytes [42]. It has also been shown that phthalocyanine and sodium ascorbate significantly slowed the growth of Ehrlich carcinoma in mice in comparison with the suppressive effect of PDT only [49]. But it's still unclear why and in what settings ascorbate realizes its pro-or antioxidant capacities.…”

Section: Introductionmentioning

confidence: 99%

Ascorbate enhances the toxicity of the photodynamic action of Verteporfin in HL-60 cells

Kramarenko

Wilke

Dayal

et al. 2006

Free Radical Biology and Medicine

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As a reducing agent, ascorbate serves as an antioxidant. However, its reducing function can in some settings initiate an oxidation cascade, i.e. appear to be a "pro-oxidant". This dichotomy also appears to hold when ascorbate is present during photosensitization. Ascorbate can react with singlet oxygen producing hydrogen peroxide. Thus, if ascorbate is present during photosensitization the formation of highly diffusible hydrogen peroxide could enhance the toxicity of the photodynamic action. On the other side, ascorbate could decrease toxicity by converting highly reactive singlet oxygen to less reactive hydrogen peroxide, which can be removed via peroxide-removing systems such as glutathione and catalase. To test the influence of ascorbate on photodynamic treatment we incubated leukemia cells (HL-60 and U937) with ascorbate and a photosensitizer (verteporfin, VP) and examined: AscH − uptake; levels of glutathione; changes in membrane permeability; cell growth; and toxicity. Accumulation of VP was similar in each cell line. Under our experimental conditions, HL-60 cells were found to accumulate less ascorbate and have lower levels of intracellular GSH compared to U937 cells. Without added ascorbate, HL-60 cells were more sensitive to VP and light treatment than U937 cells. When cells were exposed to VP and light ascorbate acted as an antioxidant in U937 cells, while it was a "pro-oxidant" for HL-60 cells. One possible mechanism to explain these observations is that HL-60 cells express myeloperoxidase activity, while in U937 cells it is below detection limit. Inhibition of myeloperoxidase activity with 4-aminobenzoic acid hydrazide (4-ABAH) had minimal influence on the phototoxicity of VP in HL-60 in the absence of ascorbate. However, 4-ABAH decreased the toxicity of ascorbate on HL-60 cells during VP-photosensitization, but had no affect on ascorbate toxicity in U937 cells. These data demonstrate that ascorbate increases hydrogen peroxide production by VP and light. This hydrogen peroxide activates MPO, producing toxic oxidants. These observations suggest that in some settings, ascorbate may enhance the toxicity of photodynamic action.

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“…Ascorbate in this approach acts to chemically activate B12 and its derivatives, or Co phthalocyanine (PcCo) to become free radical generators ( 1–12 ). Furthermore, in vivo studies using the same CT system has been successfully conducted in Russia and the USA ( 9–21 ) and clinical trials have been started in Russia ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

“…Apparently, the efficient generation of oxygen radicals by ascorbate intensifies the antitumor effect. These findings led to the hypothesis that ascorbate along with photosensitizers may act by direct tumor cell damage resulting in apoptosis ( 32 , 33 ). Recently, it has been demonstrated that CT can achieve efficiency comparable with that of PDT ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

Catalytic Therapy of Cancer with Ascorbate and Extracts of Medicinal Herbs

Rozanova

Zhang

Heck

2000

Evidence-Based Complementary and Alternative Medicine

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Catalytic therapy (CT) is a cancer treatment modality based on the generation of reactive oxygen species (ROS) using a combination of substrate molecules and a catalyst. The most frequently used substrate/catalyst pair is ascorbate/Co phthalocyanine (PcCo). In the present work, herb extracts containing pigments have been studied as a catalyst in place of PcCo. Extracts from herbs are expected to have efficiency comparable with that of phthalocyanines but as natural products, to exhibit fewer side effects. The present studies demonstrate that a combined use of ascorbate and herbal extracts results in ROS production and a significant decrease in the number of cancer cells after a single in vitro treatment. Treatment with ascorbate in conjunction with extracts prepared from several medicinal herbs stimulated apoptosis and disrupted the cell cycle. The number of cells accumulating in the sub-G0/G1 stage of the cell cycle was increased 2- to 7-fold, and cells in G2/M increased 1.5- to 20-fold, indicating that the treatment protocol was highly effective in suppressing DNA synthesis and potentially reflecting DNA damage in the tumor cells. In addition, 20–40% of the cells underwent apoptosis within 24 h of completing treatment. Our results suggest that herbal extracts can function as CT catalysts in the treatment of cancer.

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<title>Improvement of cancer PDT using sulphophthalocyanine and sodium ascorbate</title>

Cited by 4 publications

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Photothermal ablation therapy for cancer based on metal nanostructures

Photothermal ablation therapy for cancer based on metal nanostructures

Ascorbate enhances the toxicity of the photodynamic action of Verteporfin in HL-60 cells

Catalytic Therapy of Cancer with Ascorbate and Extracts of Medicinal Herbs

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