&lt;title&gt;Characterization of cervical normal and abnormal tissues by synchronous luminescence spectroscopy&lt;/title&gt;

Vengadesan, Nammalver; Anbupalam, T.; Hemamalini, Srinivasan; Ebenezar, Jeyasingh; Muthvelu, Kulandhaivel; Koteeswaran, Dornadula; Aruna, Prakasarao; Ganesan, Singaravelu

doi:10.1117/12.465247

Cited by 11 publications

(7 citation statements)

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“…Selection of optimum wavelength offset and quality of SF signal in case of multiple fluorophores depend upon two parameters, namely resolution and magnitude [23,25]. On the basis of these two parameters smaller wavelength offsets are selected as optimum offset to study behavior of multiple components in biological samples [17,18,[20][21][22][23][24][25][28][29][30]. It is observed that our technique works efficiently for lower offsets for a wide-range of concentrations of scatterers and absorbers compared to larger one, consistent with the offsets reported in literature [17,18,[20][21][22][23][24][25][28][29][30].…”

Section: Figs 1(a) and (B)mentioning

confidence: 99%

“…Peuravuori et al used synchronous fluorescence spectroscopy for differentiating different humic-solute aggregates from water sample [16]. In recent years, it has been applied in cancer diagnosis [17][18][19][20][21][22][23][24][25][26][27][28][29]. Diagaradjane et al explored the potential of this technique to discriminate the sequential tissue transformation in DMBA-TPA induced tumor model in mouse skin [30].…”

Section: Introductionmentioning

confidence: 99%

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A technique for correction of attenuations in synchronous fluorescence spectroscopy

Devi

Ghosh

Pradhan

2015

Journal of Photochemistry and Photobiology B: Biology

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Section: Figs 1(a) and (B)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A technique for correction of attenuations in synchronous fluorescence spectroscopy

Devi

Ghosh

Pradhan

2015

Journal of Photochemistry and Photobiology B: Biology

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“…Recently, synchronous fluorescence spectroscopy has been used in the diagnosis of cancerous and precancerous lesions [8][9][10][11]. This technique was introduced by Lloyd [12] and basic theory was provided by Vo-Dinh [13][14].…”

Section: Introductionmentioning

confidence: 99%

Extraction of masked fluorescence peaks through synchronous fluorescence spectroscopy

et al. 2012

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Fluorescence spectroscopy has been demonstrated as a viable tool for noting subtle biochemical changes that occur during early-stage cervical cancer progression. Due to multiple fluorophore contributions, the individual fluorophore activities often get masked due to overlapping spectra of neighboring fluorophores. Recently synchronous fluorescence spectroscopy has been demonstrated as an efficient technique for investigation of such non-dominant fluorophores. With synchronous fluorescence spectroscopy individual fluorophore responses are highlighted as sharp peaks by choosing appropriate offsets during signal acquisition. Such peaks may, however be missed due to absorption effects. By correcting the measured synchronous fluorescence spectrum with elastic scattering data, it was observed that the masked fluorophores are highlighted while the broader bands are sharpened. Interestingly, fluorophore activities of protoporphyrin, collagen, NADH, FAD and porphyrin can now be studied using this technique, as compared to only collagen and NADH seen earlier. The results have been verified using tissue phantoms with known fluorophores and scatterers. Use of normalized synchronous spectra has led to enhancement of several fluorophore responses. It was also observed that among the different offsets, the lower ones show sharper features, whereas the larger offsets show a broadband response. Among the different offsets 120nm is found optimal for further investigation.

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“…Several other research groups have carried out SF spectroscopy on ex vivo tissue samples, such as the cornea [ 40 ]. SF has been used to analyze normal and abnormal cervical tissues [ 41 ], and three-dimensional, total synchronous luminescence spectroscopy criteria for discrimination between normal and malignant breast tissue [ 42 ]. Moreover, the SF method has been used to characterize DMBA-TPA-induced squamous cell carcinoma in mice in vivo [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis

Zhang

Fales

Vo‐Dinh

2015

Sensors

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This article presents our most recent advances in synchronous fluorescence (SF) methodology for biomedical diagnostics. The SF method is characterized by simultaneously scanning both the excitation and emission wavelengths while keeping a constant wavelength interval between them. Compared to conventional fluorescence spectroscopy, the SF method simplifies the emission spectrum while enabling greater selectivity, and has been successfully used to detect subtle differences in the fluorescence emission signatures of biochemical species in cells and tissues. The SF method can be used in imaging to analyze dysplastic cells in vitro and tissue in vivo. Based on the SF method, here we demonstrate the feasibility of a time-resolved synchronous fluorescence (TRSF) method, which incorporates the intrinsic fluorescent decay characteristics of the fluorophores. Our prototype TRSF system has clearly shown its advantage in spectro-temporal separation of the fluorophores that were otherwise difficult to spectrally separate in SF spectroscopy. We envision that our previously-tested SF imaging and the newly-developed TRSF methods will combine their proven diagnostic potentials in cancer diagnosis to further improve the efficacy of SF-based biomedical diagnostics.

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<title>Characterization of cervical normal and abnormal tissues by synchronous luminescence spectroscopy</title>

Cited by 11 publications

References 0 publications

A technique for correction of attenuations in synchronous fluorescence spectroscopy

A technique for correction of attenuations in synchronous fluorescence spectroscopy

Extraction of masked fluorescence peaks through synchronous fluorescence spectroscopy

Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis

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