&lt;title&gt;Application of 5-ALA for differential diagnostics of stomach diseases&lt;/title&gt;

Охотникова, Н. Л.; Dadvany, Sergey A.; Kuszin, Michail I.; Kharnas, Sergey S.; Zavodnov, Victor Ya.; Sklyanskaya, Olga A.; Loschenov, Victor B.; Volkova, Anna I.; Agafonov, Valery V.

doi:10.1117/12.413712

Cited by 1 publication

(1 citation statement)

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“…However, the group of Prof. Loshenov has found and recommended the most optimal regime of°uorescence observations after application of 5-ALA, namely, the time period from 2 to 4 h after administration and a dose of the drug of 20 mg/kg for optimal discrimination of di®erent stomach lesions, including those with carcinoma. 18 In all experiments, an excitation source at 405 nm was used to detect the exogenous°uorescence signal from photosensitizers. It is based on an light-emitting diode (LED) source AFS-405 (Polironik Ltd., Moscow, Russia), with FWHM = 20 nm and an output light power of 25 mW.…”

Section: Experimental Studiesmentioning

confidence: 99%

Photodynamic diagnostics of early gastric cancer: Complexity measures of gastric microcirculation and new model of metastatic adenocarcinoma of rat stomach

Павлов

Borisova

Pavlova

et al. 2019

J. Innov. Opt. Health Sci.

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The detection of early gastric cancer that often develops asymptomatically is crucial for improving patient survival. The photodynamic diagnosis (PDD) of gastric cancer using 5-aminolevulinic acid/protoporphyrin IX (5-ALA/PpIX) has been reported in several studies. However, the selectivity of PDD of gastric tumor is poor with often false-positive results that require the development of new methods to improve PDD for early gastric cancer. Therefore, a measure of the complexity of gastric microcirculation (multi-scale entropy, MSE) and the detrended fluctuation analysis (DFA) were applied as additional tools to detect early gastric cancer in rats.In this experimental study, we used our original model of metastatic adenocarcinoma in the stomach of a rat. To induce a gastric tumor, we used a long-term combination (for 9 months, which is 1/2 of the life of rats) of two natural factors, such as chronic stress (overpopulation being typical for modern cities) and the daily presence of nitrites in food and drinks, which are common ingredients added to processed meat and fish to help preserve food. Our results clearly show that both methods, namely, PDD using 5-ALA/PpIX and complexity/correlation analysis, can detect early gastric cancer, which was confirmed by histological analysis. Pre-cancerous areas in the stomach were detected as an intermediate fluorescent signal or MSE level between normal and malignant lesions of the stomach. However, in some cases, PDD with 5-ALA/PpIX produced a false-positive fluorescence of exogenous fluorophores due to its accumulation in benign and inflammatory areas of the mucosa. This fact indicates that the PDD itself is not sufficient for the correct diagnosis of gastric cancer, and the use of additional characteristics, e.g., complexity measures or scaling exponents, can significantly improve the diagnostic accuracy of PDD of gastric cancer that should be confirmed in further clinical studies and applications.

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Section: Experimental Studiesmentioning

confidence: 99%