&lt;p&gt;ZNRD1-AS1 Promotes Nasopharyngeal Carcinoma Cell Invasion and Metastasis by Regulating the miR-335–ROCK1 Axis&lt;/p&gt;

Wang, Qiang; Hu, Xinyu; Du, Mengnan; Lü, Zhiwei; Yan, Keshi; Zhao, Dingliang; Jiang, Ning; Peng, Yi; He, Xia; Li, Yin

doi:10.2147/ott.s250028

Cited by 8 publications

(9 citation statements)

References 31 publications

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“…For example, LINC00152 drive ANXA2-mediated invasion and metastasis of NPC cells via sponging miR-613 [19]. Another lncRNA ZNRD1-AS1, up-regulated in NPC and associated with advanced TNM stages, was also reported increased the NPC cell invasiveness and metastasis via affecting miR-335-ROCK1 axis [21]. In some other cancers, Wang and colleagues showed that the EPB41L4A-AS2-miR-301a-5p-FOXL1 axis inhibits hepatocellular carcinoma development [39].…”

Section: Discussionmentioning

confidence: 99%

LncRNA EPB41L4A-AS2 represses Nasopharyngeal Carcinoma Metastasis by binding to YBX1 in the Nucleus and Sponging MiR-107 in the Cytoplasm

Jiang

et al. 2021

Int. J. Biol. Sci.

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Nasopharyngeal carcinoma (NPC) is known for its potential to progress to the lymph nodes and distant metastases at an early stage. As an important regulator in tumorigenesis biological processes, the functions of lncRNA in NPC tumor development remain largely unclear. In this research, the expression of EPB41L4A-AS2 in NPC tissues and cells was analyzed via real-time quantitative polymerase chain reaction (qRT-PCR). CCK8, colony formation, and EDU experiments were used to determine the viability of NPC cells. Transwell and wound healing assays were performed to test NPC cell migration and invasion. RNA pull-down and mass spectrometry analysis were used to identify potential binding proteins. Then, a popliteal lymph node metastasis model was established to test NPC metastasis. EPB41L4A-AS2 is repressed by transforming growth factor-beta, which is downregulated in NPC cells and tissue. It is associated with the presence of distant metastasis and adverse outcomes. The univariate and multivariate survival assays confirmed that EPB41L4A-AS2 expression was an independent predictor of progression-free survival (PFS) in patients with NPC. Biological analyses showed that overexpression of EPB41L4A-AS2 reduced the metastasis and invasion of NPC in vitro and in vivo , but had no significant effect on cell proliferation. Mechanistically, in the nucleus we identified that EPB41L4A-AS2 relies on binding to YBX1 to reduce the stability of Snail mRNA to enhance the expression of E-cadherin and reverse the progression of epithelial-to-mesenchymal transition (EMT). In the cytoplasm, we found that EPB41L4A-AS2 blocked the invasion and migration of NPC cells by promoting LATS2 expression via sponging miR-107. In a whole, the findings of this study help to further understand the metastasis mechanism of NPC and could help in the prevention and treatment of NPC metastasis.

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Section: Discussionmentioning

confidence: 99%

LncRNA EPB41L4A-AS2 represses Nasopharyngeal Carcinoma Metastasis by binding to YBX1 in the Nucleus and Sponging MiR-107 in the Cytoplasm

Jiang

et al. 2021

Int. J. Biol. Sci.

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“… 24 In nasopharyngeal carcinoma, miR-335 inhibits invasion and metastasis of cancer cells by directly regulating ROCK1. 72 Besides, miR-335 inhibits GC cell migration and invasion via regulating ROCK1. 58 MiR-335 acts as a tumor suppressor to modulate cell migration and invasion by the down-regulation of ROCK1 in HCC.…”

Section: Biological Role Of Mir-335 In Various Cancersmentioning

confidence: 99%

“… [ 58 ] LncRNA NEAT1 HCC [ 82 ] LncRNA ZEB1-AS1 Gastric cancer. [ 42 ] LncRNA DANCR Osteosarcoma [ 59 ] LncRNA DANCR Cervical cancer [ 56 ] LncRNA SLCO4A1-AS1 Bladder cancer [ 48 ] LncRNA CASC9 NSCLC [ 29 ] LncRNA LINC01510 Thyroid carcinoma [ 65 ] LncRNA TINCR Ovarian cancer [ 113 ] LncRNA XIST NSCLC [ 64 ] LncRNA XIST Bladder cancer [ 110 ] lncRNA ZNRD1-AS1 Nasopharyngeal Carcinoma [ 72 ] CircRNA Circ-EIF4G3 Gastric cancer [ 70 ] Circ_103973 Cervical Cancer [ …”

Section: Factors Modulating Mir-335 In Cancersmentioning

confidence: 99%

“…LncRNA ZNRD1-AS1 promotes cell invasion and metastasis by regulating the miR-335–ROCK1 axis in nasopharyngeal carcinoma (NPC). 72 Sry-related HMG box 2 (SOX2) modulates LncRNA LINC01510/miR-335/SHH axis to promote thyroid carcinoma progression. 65 LncRNA CASC9 expression is elevated in NSCLC and promotes NSCLC progression by regulating the miR-335/S100A14 axis.…”

Section: Factors Modulating Mir-335 In Cancersmentioning

confidence: 99%

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Functions and Targets of miR-335 in Cancer

Ye¹,

Wang²,

Wu³

et al. 2021

OTT

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MicroRNAs (miRNAs) are small non-coding RNAs (18~25 nt in length) that act as master regulators of eukaryotic gene expression. They might play an oncogenic or tumorsuppressive role in multiple cancers. In recent decades, several studies have focused on the functions and mechanisms of miR-335 in cancer. The expression level of miR-335 in tissues and cells varies with cancer types, and miR-335 has been proposed as a potential biomarker for the prognosis of cancer. Besides, miR-335 may serve as an oncogene or tumor suppressor via regulating different targets or pathways in tumor initiation, development, and metastasis. Furthermore, miR-335 also influences tumor microenvironment and drug sensitivity. MiR-335 is regulated by various factors such as lncRNAs and microRNAs. In this review, we reveal the functions and targets of miR-335 in various cancers and its potential application as a possible biomarker in prognostic judgment and treatment of malignant tumors.

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“…FTH1P3 enhances the proliferation, migration and invasion of oral squamous cell carcinoma and laryngeal squamous cell carcinoma [ 29 , 30 ]. Zinc ribbon domain containing 1 antisense 1 ( ZNRD1-AS1 ) accelerates invasion and metastasis of NPC cells [ 31 ]. Besides, pseudogenes contribute to HNSCC progression in vivo [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of key pseudogenes in nasopharyngeal carcinoma based on RNA-Seq analysis

et al. 2021

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Background Nasopharyngeal carcinoma (NPC) is a malignant head and neck tumor, and more than 70% of new cases are in East and Southeast Asia. However, association between NPC and pseudogenes playing important roles in genesis of multiple tumor types is still not clear and needs to be investigated. Methods Using RNA-Sequencing (RNA-seq) technology, we analyzed pseudogene expression in 13 primary NPC and 6 recurrent NPC samples as well as their paracancerous counterparts. Quantitative PCR was used to validate the differentially expressed pseudogenes. Results We found 251 differentially expressed pseudogenes including 73 up-regulated and 178 down-regulated ones between primary NPC and paracancerous tissues. Enrichment analysis of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were conducted to filter out the key pseudogenes. We reported that pseudogenes from cytochrome P450 (CYP) family, such as CYP2F2P, CYP2G1P, CYP4F24P, CYP2B7P and CYP2G2P were significantly down-regulated in NPC compared to paracancerous tissues, while IGHV1OR15–2, IGHV3–11, FCGR1CP and IGHV3–69-1 belonging to Fc gamma receptors were significantly up-regulated. CYP2B7P, CYP2F2P and CYP4F26P were enriched in arachidonic acid metabolism pathway. The qRT-PCR analysis validated the lower expression of pseudogenes CYP2F2P and CYP2B7P in NPC tissues and cell lines compared to paracancerous tissues and normal human nasopharyngeal epithelial cell line. CYP2B7P overexpression weakened migratory and invasive capacity of NPC cell line. Moreover, the expression pattern of those pseudogenes in recurrent NPC tissues was different from the primary NPC. Conclusion This study suggested the role of pseudogenes in tumorigenesis and progression, potentially functioning as therapeutic targets to NPC.

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<p>ZNRD1-AS1 Promotes Nasopharyngeal Carcinoma Cell Invasion and Metastasis by Regulating the miR-335–ROCK1 Axis</p>

Cited by 8 publications

References 31 publications

LncRNA EPB41L4A-AS2 represses Nasopharyngeal Carcinoma Metastasis by binding to YBX1 in the Nucleus and Sponging MiR-107 in the Cytoplasm

LncRNA EPB41L4A-AS2 represses Nasopharyngeal Carcinoma Metastasis by binding to YBX1 in the Nucleus and Sponging MiR-107 in the Cytoplasm

Functions and Targets of miR-335 in Cancer

Identification of key pseudogenes in nasopharyngeal carcinoma based on RNA-Seq analysis

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