<p>The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface</p>

Abstract

Purpose: Zirconia is one of the most promising implant materials due to its favorable physical, mechanical and biological properties. However, until now, we know little about the mechanism of osseointegration on zirconia. The purpose of this study is to evaluate the effect of Syndecan (Sdc) on osteoblastic cell (MC3T3-E1) adhesion and proliferation onto zirconia materials. Materials and Methods: The mirror-polished disks 15 mm in diameter and 1.5 mm in thick of commercial pure titanium (CpTi), 3mol% yttria-stabilized tetragonal zirconia polycrystalline (3Y-TZP) and nano-zirconia (NanoZr) are used in this study. MC3T3-E1 cells were seeded onto specimen surfaces and subjected to RNA interference (RNAi) for Syndecan-1, Syndecan-2, Syndecan-3, and Syndecan-4. At 48h post-transfection, the cell morphology, actin cytoskeleton, and focal adhesion were observed using scanning electron microscopy or laser scanning confocal fluorescence microscopy. At 24h and 48h posttransfection, cell counting kit-8 (CCK-8) assay was used to investigate cell proliferation. Results: The cell morphology of MC3T3-E1 cells on CpTi, 3Y-TZP, and NanoZr changed into abnormal shape after gene silencing of Syndecan. Among the Syndecan family, Sdc-2 is responsible for NanoZr-specific morphology regulation, via maintenance of cytoskeletal conformation without affecting cellular attachment. According to CCK-8 assay, Sdc-2 affects the osteoblastic cell proliferation onto NanoZr. Conclusion: Within the limitation of this study, we suggest that Syndecan affects osteoblastic cell adhesion on CpTi, 3Y-TZP, and NanoZr. Sdc-2 might be an important heparin-sensitive cell membrane regulator in osteoblastic cell adhesion, specifically on NanoZr, through the organization of actin cytoskeleton and affects osteoblastic cell proliferation.

Antibacterial Zirconia Surfaces from Organocatalyzed Atom-Transfer Radical Polymerization

“…Zirconia (ZrO 2 ), a crystalline oxide of zirconium, has emerged as a promising and appropriate ceramic biomaterial for implant manufacturing, and it is one of the most important alternatives to titanium and titanium alloys [1,2]. Due to aesthetic advantages, such as color and opacity that imitate natural teeth appearance, dense zirconia or yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) has been widely employed as multi-unit bridges, crowns, onlays, inlays, and implant structures [3].…”

Promoted Abutment-Soft Tissue Integration Around Self-Glazed Zirconia Surfaces with Nanotopography Fabricated by Additive 3D Gel Deposition

“…Many studies have proven that nanotopography can not only improve the biological activities of surfaces but also play a certain role in the regulation of bacterial adhesion. [25][26][27][28][29][30] Moreover, this material exhibits an enamel-like outer surface that is smoother than that of conventional zirconia and can be used directly without any further manual processing. It showed a higher accuracy and fitness as well as reduced wear to tooth enamel, 26,31 and was widely used in the clinic for crown restoration, achieving a high level of evaluation by physicians and patients.…”